##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512236_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2885129 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.189814042976934 31.0 30.0 34.0 25.0 34.0 2 26.25338866996935 31.0 26.0 34.0 2.0 34.0 3 27.938128243139214 31.0 27.0 34.0 16.0 34.0 4 32.47192274591535 35.0 32.0 37.0 25.0 37.0 5 32.893702153352585 35.0 33.0 37.0 26.0 37.0 6 33.103960342847756 35.0 35.0 37.0 27.0 37.0 7 33.09632047648476 36.0 35.0 37.0 26.0 37.0 8 33.10118230415347 37.0 35.0 37.0 26.0 37.0 9 34.58494299561649 39.0 35.0 39.0 27.0 39.0 10 34.433400724889594 38.0 35.0 39.0 27.0 39.0 11 34.45059267713852 38.0 35.0 39.0 27.0 39.0 12 34.33102748611934 38.0 35.0 39.0 27.0 39.0 13 34.32967191415011 38.0 35.0 39.0 27.0 39.0 14 35.34376833756827 39.0 35.0 41.0 26.0 41.0 15 35.282693772098234 39.0 35.0 41.0 26.0 41.0 16 35.22601866329027 39.0 35.0 41.0 26.0 41.0 17 35.20850956750981 39.0 35.0 41.0 26.0 41.0 18 35.15504159432732 39.0 35.0 41.0 25.0 41.0 19 35.17346226113286 39.0 35.0 41.0 25.0 41.0 20 35.083526247873145 39.0 35.0 41.0 25.0 41.0 21 34.995532608767235 39.0 35.0 41.0 25.0 41.0 22 34.88592607124326 39.0 34.0 41.0 24.0 41.0 23 34.76111674729275 39.0 34.0 41.0 24.0 41.0 24 34.64101154575757 39.0 34.0 41.0 23.0 41.0 25 34.50664944271123 39.0 34.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4892735837312081 1101 2 3.5274036990199704 1101 3 2.0447249979679114 1101 4 0.9263914225814531 1101 5 0.7047177971911012 1101 6 0.5482361208889301 1101 7 0.37032109978457584 1101 8 0.4059342857155386 1101 9 0.35555846877688424 1101 10 0.37890260798855024 1101 11 0.49837764832614084 1101 12 0.42982930238487427 1101 13 0.55075912486204 1101 14 0.54844594680835 1101 15 0.48267014138060205 1101 16 0.654041973463805 1101 17 0.4428166542690093 1101 18 0.4924805169644628 1101 19 0.26357489513731025 1101 20 0.5201815636706044 1101 21 0.4931955930662255 1101 22 0.5125501003439226 1101 23 0.5810059518665156 1101 24 0.5885140921219971 1101 25 0.6856790923757288 1102 1 1.1949937597082751 1102 2 4.0494672852524864 1102 3 2.426855573407721 1102 4 1.558857005023519 1102 5 1.0571229449254318 1102 6 1.101342131316251 1102 7 0.9855661504126019 1102 8 0.8308594569853724 1102 9 0.87093586935557 1102 10 0.9224854914508214 1102 11 0.8494783991672321 1102 12 1.0304975687034883 1102 13 0.9544404287820427 1102 14 1.0021200093475926 1102 15 1.1451184271513597 1102 16 1.0997415755197864 1102 17 1.1950147079972098 1102 18 1.236169372513345 1102 19 1.1965985583980654 1102 20 1.076010035208185 1102 21 1.2056990935185183 1102 22 1.0354172156555634 1102 23 0.9362052293812866 1102 24 1.039757380974244 1102 25 0.9864355152230502 1103 1 1.0156231267689222 1103 2 3.866550139118033 1103 3 2.436965026841925 1103 4 1.494995428823934 1103 5 1.0696855025498948 1103 6 0.9014670699134015 1103 7 0.7069939049435874 1103 8 0.7990902725381588 1103 9 0.8008344293688907 1103 10 0.8798378295481726 1103 11 0.7900144051152935 1103 12 0.7900221739391284 1103 13 0.8328504948445712 1103 14 0.9419461369027289 1103 15 1.0508214527642963 1103 16 0.9999981000546256 1103 17 1.0362588127352197 1103 18 0.9349485387013061 1103 19 1.0784953377615807 1103 20 1.092292617317888 1103 21 1.1447860315728988 1103 22 1.2475430116026232 1103 23 1.2514443622226281 1103 24 1.3857744855759009 1103 25 1.25473552592684 1104 1 0.8438118350215724 1104 2 3.6773785822978304 1104 3 2.100936859959887 1104 4 1.2872510952791245 1104 5 0.7929875524791186 1104 6 0.5970512090942535 1104 7 0.557877602646478 1104 8 0.4087461349766741 1104 9 0.6371229769675182 1104 10 0.49622926713387017 1104 11 0.39142584210253517 1104 12 0.4677804807880577 1104 13 0.6403298209174224 1104 14 0.647138588552508 1104 15 0.4647164037923517 1104 16 0.7118589886079079 1104 17 0.620650076314682 1104 18 0.6184749510623817 1104 19 0.6129671798061622 1104 20 0.6799114199488017 1104 21 0.672970012488534 1104 22 0.8481589735566146 1104 23 0.8535836330912474 1104 24 0.9053686688391664 1104 25 0.6991021872375356 1105 1 0.76860558819196 1105 2 3.623455726395008 1105 3 2.2839155677788625 1105 4 1.3784110036423556 1105 5 1.0148291146260249 1105 6 0.6569623192286826 1105 7 0.3843636328001807 1105 8 0.5001283595673414 1105 9 0.5528244725660585 1105 10 0.5095689732882178 1105 11 0.7327134815766669 1105 12 0.48278078294767823 1105 13 0.6207674579193352 1105 14 0.6711637575329874 1105 15 0.7053035823357234 1105 16 0.6710864854993233 1105 17 0.6621027132525086 1105 18 0.7647700163380193 1105 19 0.6831260791393987 1105 20 0.5679490122677251 1105 21 0.734312443573252 1105 22 0.6521093900790689 1105 23 0.5000191568071131 1105 24 0.5879010960599089 1105 25 0.5966719082801077 1106 1 0.29095716986180875 1106 2 3.1756175525112518 1106 3 1.556216686189142 1106 4 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0.6345382275744882 1107 16 0.7365741695201891 1107 17 0.5788126858784253 1107 18 0.6881881115121473 1107 19 0.6903664820085496 1107 20 0.7163987767657929 1107 21 0.8539200856904472 1107 22 0.8166345623307265 1107 23 0.8022608146652885 1107 24 0.7572090138033261 1107 25 0.9502465058882095 1108 1 0.6267121581586679 1108 2 3.1974052387041922 1108 3 1.9302092274225267 1108 4 1.0766792720321376 1108 5 0.6171109977779352 1108 6 0.4843898910874245 1108 7 0.5218389707846853 1108 8 0.4524477885242604 1108 9 0.4869388973810871 1108 10 0.3365142015025455 1108 11 0.45172472335141833 1108 12 0.4200902141417373 1108 13 0.4004387312452806 1108 14 0.44823925437179923 1108 15 0.4902857227876751 1108 16 0.457132510470899 1108 17 0.5783562026422757 1108 18 0.44632401379565323 1108 19 0.5151886358266253 1108 20 0.5221567726590095 1108 21 0.565835774287919 1108 22 0.5726191112342605 1108 23 0.531404328208481 1108 24 0.6504754723052883 1108 25 0.5036710359688001 1109 1 0.9623813424167338 1109 2 3.425086248976811 1109 3 2.0453037418256557 1109 4 1.105655361319755 1109 5 0.814793277714692 1109 6 0.7144933039359955 1109 7 0.5238400953443474 1109 8 0.4758531184361203 1109 9 0.5413667862880658 1109 10 0.3396460397127896 1109 11 0.40168872794121313 1109 12 0.5815329758253469 1109 13 0.5599490338788016 1109 14 0.6273054077601827 1109 15 0.6076973742449994 1109 16 0.5334059641689635 1109 17 0.6162616152550484 1109 18 0.7060070382565584 1109 19 0.5380050186398648 1109 20 0.6498478452358611 1109 21 0.5809951970312284 1109 22 0.5466297556838242 1109 23 0.5853249992475753 1109 24 0.5681880643937305 1109 25 0.47617114717237286 1110 1 0.8907220254343073 1110 2 3.690342010056341 1110 3 2.073173782041227 1110 4 1.233654568948232 1110 5 0.7969412265871085 1110 6 0.6215131186513076 1110 7 0.6526034947935315 1110 8 0.5418371474324104 1110 9 0.555256353577235 1110 10 0.42245512618523406 1110 11 0.46402265033472645 1110 12 0.28364282156338305 1110 13 0.6976364236909376 1110 14 0.6857594024106319 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-1.5757277189362213 2315 19 -1.8678539569751607 2315 20 -1.668136963245665 2315 21 -1.7312664149511647 2315 22 -1.5962814272734036 2315 23 -1.6687010629738452 2315 24 -1.4843697160468778 2315 25 -1.5613881279689537 2316 1 -1.3710925947461376 2316 2 -0.12899589561505564 2316 3 -0.4354311368715855 2316 4 -1.0711475632398972 2316 5 -1.093842992960461 2316 6 -1.2025628193607822 2316 7 -1.244185155825221 2316 8 -1.3349180935688878 2316 9 -1.2673736590426046 2316 10 -1.3917203578892767 2316 11 -1.7717900344894488 2316 12 -1.4841585713280665 2316 13 -1.5928553734121493 2316 14 -1.7509412624854903 2316 15 -1.869366626061769 2316 16 -1.6071950692691814 2316 17 -1.6613898054827345 2316 18 -1.993462262175484 2316 19 -1.8965750552410583 2316 20 -2.069822071296592 2316 21 -1.9477838504417377 2316 22 -1.814230372265662 2316 23 -1.7427552631703023 2316 24 -1.5855198822790726 2316 25 -1.4135618972839978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111402.0 3 4.0 4 1050.0 5 4966.0 6 5980.0 7 5417.0 8 5129.0 9 5096.0 10 4854.0 11 4736.0 12 4645.0 13 4577.0 14 4771.0 15 4870.0 16 5145.0 17 5662.0 18 6347.0 19 7854.0 20 10349.0 21 13366.0 22 16660.0 23 19851.0 24 23348.0 25 26645.0 26 30747.0 27 34451.0 28 40249.0 29 48129.0 30 59793.0 31 76788.0 32 98592.0 33 128323.0 34 171590.0 35 235623.0 36 333788.0 37 418623.0 38 905709.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16327935423338 19.327870608211974 13.498321912122474 25.010528125432174 2 15.135320261202539 20.760522825212767 41.82050277887813 22.28365413470657 3 18.691781199384845 24.49706754879938 29.897796597656466 26.91335465415931 4 11.897007031574672 17.038475575962114 39.12466305666055 31.939854335802664 5 12.700887897906817 38.18349196864334 36.359344764133596 12.756275369316242 6 31.05465520880739 37.758459518017084 18.56738863473065 12.619496638444877 7 27.600094698578097 31.71359018185108 22.46172628020727 18.224588839363552 8 23.989395274873324 38.878573540385894 20.83147755265016 16.30055363209063 9 25.91509773046543 15.86234099064548 20.963187434599977 37.259373844289115 10 14.692746589375705 29.72316742743314 34.951607072233934 20.63247891095722 11 33.647421828532295 22.440337628090568 25.65762748053079 18.254613062846353 12 23.3320566699883 24.56690784628049 32.679242667128804 19.4217928166024 13 28.826267387004183 22.53507555468057 26.174080950973078 22.464576107342168 14 19.96290623512844 23.369037208491427 28.035184659499553 28.63287189688058 15 22.0766558445047 32.82986653283094 23.46945318562879 21.624024437035573 16 20.891682832899324 28.711298524260094 29.58550553545439 20.811513107386187 17 20.60161628877471 29.529365768173893 29.010116708860057 20.858901234191343 18 20.34594759483228 28.084185203287543 31.75476544545086 19.81510175642932 19 22.95701272772648 27.204614147464163 27.94734215123534 21.891030973574026 20 22.8767587168546 28.28331072891368 29.081923199967836 19.75800735426388 21 22.019391051249265 27.448240673372993 27.684606156317344 22.847762119060402 22 22.207279495613655 29.40941183810557 28.054376119982393 20.328932546298383 23 21.38266507594627 28.819479346399703 28.6027425369125 21.19511304074153 24 22.597433569635715 27.270064470999145 29.592705095527933 20.53979686383721 25 22.02884695708659 28.207084423329494 29.08051407494552 20.683554544638397 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 329.0 1 329.0 2 463.5 3 598.0 4 598.0 5 598.0 6 1519.5 7 2441.0 8 2441.0 9 2441.0 10 3764.5 11 5088.0 12 5088.0 13 5088.0 14 9193.0 15 13298.0 16 13298.0 17 13298.0 18 24423.5 19 35549.0 20 35549.0 21 35549.0 22 58673.0 23 81797.0 24 81797.0 25 81797.0 26 120724.0 27 159651.0 28 159651.0 29 159651.0 30 210397.0 31 261143.0 32 261143.0 33 261143.0 34 287983.0 35 314823.0 36 314823.0 37 314823.0 38 331334.5 39 347846.0 40 347846.0 41 347846.0 42 361086.5 43 374327.0 44 374327.0 45 374327.0 46 373647.5 47 372968.0 48 372968.0 49 372968.0 50 350353.0 51 327738.0 52 327738.0 53 327738.0 54 282660.0 55 237582.0 56 237582.0 57 237582.0 58 200531.0 59 163480.0 60 163480.0 61 163480.0 62 131302.5 63 99125.0 64 99125.0 65 99125.0 66 75339.5 67 51554.0 68 51554.0 69 51554.0 70 37149.0 71 22744.0 72 22744.0 73 22744.0 74 15708.5 75 8673.0 76 8673.0 77 8673.0 78 5898.0 79 3123.0 80 3123.0 81 3123.0 82 2005.0 83 887.0 84 887.0 85 887.0 86 578.5 87 270.0 88 270.0 89 270.0 90 167.5 91 65.0 92 65.0 93 65.0 94 45.0 95 25.0 96 25.0 97 25.0 98 15.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.601219910790817 3 0.0 4 0.0 5 0.0 6 2.079629714997146E-4 7 0.006446852116491151 8 0.0 9 0.0 10 0.0034660495249952427 11 0.0021142902102470984 12 1.386419809998097E-4 13 0.0 14 2.772839619996194E-4 15 0.0 16 0.0 17 3.466049524995243E-5 18 0.010363488079735776 19 0.0030847840772457663 20 0.0 21 0.006585494097490961 22 0.0013517593147481446 23 0.002183611200747003 24 0.009219691736487345 25 0.006238889144991437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2885129.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.05592232320689 #Duplication Level Percentage of deduplicated Percentage of total 1 86.45088922253773 63.15749447813877 2 9.614895260286428 14.048500825625105 3 2.075128962260837 4.548013808326938 4 0.7179919148685601 2.098142462453124 5 0.33772280544558425 1.2336325520704057 6 0.18635848148615408 0.8168754448633955 7 0.12324801868550009 0.6302798375703931 8 0.08432567595414124 0.4928392025886125 9 0.05784457746103827 0.3803300062031101 >10 0.30710041090285345 3.9912641912135447 >50 0.02185908608740637 1.091880321703457 >100 0.018200714601591516 2.652675996835818 >500 0.002121024506594177 1.0195229490967217 >1k 0.002072819403562725 2.6292774909114183 >5k 2.4102551204217729E-4 1.2092704323990928 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 8548 0.2962779133965933 No Hit CTGTAGGACGTGGAATATGGCAAGA 7556 0.2618947021086406 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6910 0.23950402217717126 No Hit CTTTAGGACGTGAAATATGGCGAGG 6038 0.20928007031921275 No Hit GTATCAACGCAGAGTACTTTTTTTT 5286 0.18321537789124853 No Hit GTCCTACAGTGTGCATTTCTCATTT 3531 0.12238620872758203 No Hit GGTATCAACGCAGAGTACTTTTTTT 3254 0.11278525154334519 No Hit TATCAACGCAGAGTACTTTTTTTTT 3129 0.10845268963710114 No Hit CTGAAGGACCTGGAATATGGCGAGA 3054 0.10585315249335471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.466049524995243E-5 0.0 4 0.0 0.0 0.0 3.466049524995243E-5 0.0 5 0.0 0.0 0.0 3.466049524995243E-5 0.0 6 0.0 0.0 0.0 3.466049524995243E-5 0.0 7 0.0 0.0 0.0 6.932099049990485E-5 0.0 8 0.0 0.0 0.0 6.932099049990485E-5 0.0 9 0.0 0.0 0.0 1.0398148574985728E-4 0.0 10 0.0 0.0 0.0 1.0398148574985728E-4 0.0 11 0.0 0.0 0.0 1.0398148574985728E-4 0.0 12 0.0 0.0 0.0 1.0398148574985728E-4 3.466049524995243E-5 13 0.0 0.0 0.0 1.0398148574985728E-4 3.466049524995243E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 110 3.6379788E-12 15.762242 1 TACCGTC 40 0.005570227 14.135718 7 TAGGACC 1095 0.0 12.823269 4 GCGTATA 70 8.238202E-4 11.656111 1 GACTGCG 90 8.166522E-6 11.517991 7 TTCAACG 80 1.9538657E-4 11.473986 2 TCCAACG 425 0.0 11.309553 18 TGTAGGA 4160 0.0 11.057195 2 AGTACCG 60 0.0062406403 10.994447 5 GGTATCA 1670 0.0 10.8708935 1 AGGACGT 4350 0.0 10.831968 5 ACTCCGT 70 0.0015957017 10.77007 6 GTCTTAG 335 0.0 10.655773 1 TTAGGAC 2575 0.0 10.649822 3 CTGTAGG 4140 0.0 10.617902 1 GGACGTG 4340 0.0 10.574645 6 GGCGAGG 1885 0.0 10.549584 19 CCAACGA 295 0.0 10.542805 19 GTAGGAC 4180 0.0 10.438337 3 GACGTGG 2260 0.0 10.257777 7 >>END_MODULE