FastQCFastQC Report
Thu 26 May 2016
SRR1512236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885129
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT91760.3180447044135635No Hit
CTGTAGGACGTGGAATATGGCAAGA84160.29170272802359964No Hit
GTCCTAAAGTGTGTATTTCTCATTT72540.2514272325431549No Hit
CTTTAGGACGTGAAATATGGCGAGG71090.24640146073191183No Hit
GTATCAACGCAGAGTACTTTTTTTT70210.243351337149916No Hit
TATCAACGCAGAGTACTTTTTTTTT45620.15812117933028297No Hit
GGTATCAACGCAGAGTACTTTTTTT45590.15801719784453314No Hit
GTCCTACAGTGTGCATTTCTCATTT38130.1321604683880686No Hit
CTGAAGGACCTGGAATATGGCGAGA37560.13018482015882132No Hit
TTGTAGAACAGTGTATATCAATGAG33130.11483022076309239No Hit
CTGTAGGACCTGGAATATGGCGAGA30890.10706626982710304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12250.012.330864
CGTGCGC802.8774459E-511.87132810
GGCGAGG19100.011.28767219
CCAACGA3200.010.98154919
TCCAACG4600.010.73580918
TGTAGGA44800.010.6877152
GGTATCA19450.010.4474571
AGGACGT47000.010.4097835
GGACGTG46350.010.4088626
GTAGGAC44400.010.3560643
GACGTGG23950.010.2703097
TGTAGAA16500.010.2486912
CTGTAGG44250.010.2191711
ATAGGAC3550.010.1692183
CGGTTTC3300.010.07264213
TTAGGAC27650.010.0327513
TAGGACA11200.010.009134
AGGACCT26500.09.9662385
ACCTTTT10400.09.95365215
TAGGACG51250.09.9358094
Produced by FastQC (version 0.11.5)