FastQCFastQC Report
Thu 26 May 2016
SRR1512235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709852
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80960.2987617035911924No Hit
CTGTAGGACGTGGAATATGGCAAGA67180.24791021797500382No Hit
GTCCTAAAGTGTGTATTTCTCATTT55150.2035166496177651No Hit
CTTTAGGACGTGAAATATGGCGAGG49630.18314653346382018No Hit
GTATCAACGCAGAGTACTTTTTTTT49110.1812276094783036No Hit
GTCCTACAGTGTGCATTTCTCATTT38990.14388239652940454No Hit
GGTATCAACGCAGAGTACTTTTTTT30220.11151900546598117No Hit
TATCAACGCAGAGTACTTTTTTTTT28680.10583603827810523No Hit
CTGTAGGACCTGGAATATGGCGAGA28000.10332667614319896No Hit
CTGAAGGACCTGGAATATGGCGAGA27850.10277314037814612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11600.013.239644
TCCAACG4300.012.49157118
CCAACGA2800.012.11588519
GGCGAGG14550.011.72266219
GTCCTAC40750.011.3301271
TGTAGGA36600.011.1914722
AGGACGT35000.011.0372885
TCCTACA44100.011.0021872
GTACCGT600.00624903510.992426
GGACGTG34400.010.9559016
TTAGGAC20250.010.9342593
CCCGTTT3550.010.88263612
ACTATCG1303.6947313E-810.87082211
CTGTAGG35500.010.7613621
AGGACCT24150.010.6900575
GACGTGA17600.010.6533697
CCGTTTC3200.010.60061613
GTAGGAC36950.010.5568043
CCTACAG46400.010.4983033
ATTTCTA30200.010.48399915
Produced by FastQC (version 0.11.5)