##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512235_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2709852 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.014157599750835 31.0 29.0 34.0 23.0 34.0 2 26.05815963381026 31.0 25.0 34.0 2.0 34.0 3 27.754669996737828 31.0 26.0 34.0 16.0 34.0 4 32.27028413359844 35.0 32.0 37.0 23.0 37.0 5 32.692393163907106 35.0 33.0 37.0 26.0 37.0 6 32.90477487331412 35.0 33.0 37.0 26.0 37.0 7 32.893597510122326 36.0 33.0 37.0 26.0 37.0 8 32.89498430172571 36.0 33.0 37.0 26.0 37.0 9 34.3593059694773 39.0 35.0 39.0 27.0 39.0 10 34.21445931364517 38.0 34.0 39.0 26.0 39.0 11 34.22829401753306 38.0 35.0 39.0 26.0 39.0 12 34.10481273516044 38.0 34.0 39.0 25.0 39.0 13 34.10364994102999 38.0 34.0 39.0 25.0 39.0 14 35.1060980452069 39.0 35.0 41.0 25.0 41.0 15 35.044375855212756 39.0 34.0 41.0 25.0 41.0 16 34.97768328307229 39.0 34.0 41.0 25.0 41.0 17 34.957153748618005 39.0 34.0 41.0 25.0 41.0 18 34.90430473693766 39.0 34.0 41.0 25.0 41.0 19 34.92429734169984 39.0 34.0 41.0 24.0 41.0 20 34.828844158278756 39.0 34.0 41.0 24.0 41.0 21 34.743522155453505 39.0 34.0 41.0 23.0 41.0 22 34.635603715627276 39.0 34.0 41.0 22.0 41.0 23 34.50602542131452 39.0 34.0 41.0 19.0 41.0 24 34.379901559199546 39.0 34.0 41.0 18.0 41.0 25 34.24057771420727 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.513927260011414 1101 2 3.4957546382243976 1101 3 2.0086036672332668 1101 4 1.0363374425010718 1101 5 0.6847684370798959 1101 6 0.570859172358027 1101 7 0.4755372447387245 1101 8 0.272457694301778 1101 9 0.07365772735685994 1101 10 0.28250940605462205 1101 11 0.31662114510076833 1101 12 0.18925743425498354 1101 13 0.1597047929649804 1101 14 0.3095651105870374 1101 15 0.26757344357741175 1101 16 0.5730360545798021 1101 17 0.38974211364160993 1101 18 0.3855690647279957 1101 19 0.243685767860633 1101 20 0.43760932615994363 1101 21 0.3980113709604538 1101 22 0.4701759957302727 1101 23 0.36121804900798793 1101 24 0.38922185655734864 1101 25 0.4701503646090188 1102 1 1.3089076038644798 1102 2 4.140604644587782 1102 3 2.4319240691434345 1102 4 1.6453874881319877 1102 5 1.0849453008324517 1102 6 1.1476394974087256 1102 7 1.2004498361204554 1102 8 0.890973356225004 1102 9 0.952475998651984 1102 10 0.9099569912616374 1102 11 0.959114300463952 1102 12 1.1285301088531767 1102 13 1.0741684171018449 1102 14 1.1962290528292385 1102 15 1.2158968353195903 1102 16 1.0344390820165472 1102 17 1.0857049770500922 1102 18 1.3056032095688863 1102 19 1.0903348856523465 1102 20 1.0289893047099028 1102 21 1.2903942063188794 1102 22 1.1332534250247406 1102 23 1.2501392344779774 1102 24 1.176093200408495 1102 25 1.1863334459330872 1103 1 1.1395592178284168 1103 2 3.9598038432383973 1103 3 2.5014743148459218 1103 4 1.5937537340293346 1103 5 1.118202213688754 1103 6 0.9836846920525986 1103 7 0.8965804024844886 1103 8 0.9899527927229315 1103 9 0.9960256232478031 1103 10 1.0230334178279534 1103 11 0.9442552368735875 1103 12 1.0514644724928601 1103 13 1.099023644445154 1103 14 1.157448248678783 1103 15 1.3551309864433634 1103 16 1.1178494447550165 1103 17 1.3081634633766086 1103 18 1.092935982688367 1103 19 1.302034830239883 1103 20 1.3606228152413138 1103 21 1.4078262956120966 1103 22 1.3536357709811568 1103 23 1.3623823429341613 1103 24 1.5871653401209542 1103 25 1.4404190490459285 1104 1 1.138721602359265 1104 2 3.8750071580570626 1104 3 2.4355670466010544 1104 4 1.5711039582845387 1104 5 1.1239882950146836 1104 6 1.095259824972075 1104 7 0.9253014052468345 1104 8 0.8916859438798568 1104 9 0.9596472705582286 1104 10 0.8832615343812549 1104 11 0.8489720218559142 1104 12 0.8851888910629739 1104 13 0.9904766993654022 1104 14 1.192285032473002 1104 15 0.853280509715411 1104 16 1.015773227687859 1104 17 0.951789269061706 1104 18 0.8974923732191513 1104 19 0.8730613547297423 1104 20 0.8919413170383237 1104 21 0.9531867375879131 1104 22 1.1580213711237306 1104 23 1.1074932824298074 1104 24 1.2443905161093127 1104 25 1.0733563466964426 1105 1 0.9730299667110209 1105 2 3.5774792705789977 1105 3 2.3094050562223423 1105 4 1.5891319432560067 1105 5 1.0963147203827575 1105 6 0.778776746625212 1105 7 0.5590828686955405 1105 8 0.5948911485723443 1105 9 0.8928880094126939 1105 10 0.884621494569025 1105 11 0.9079302102830127 1105 12 0.6239842800616486 1105 13 0.8770882361815282 1105 14 0.8240089486228541 1105 15 0.8544984496653001 1105 16 0.6890175461507795 1105 17 0.7547351704100222 1105 18 0.8950000344543199 1105 19 0.9471949003173634 1105 20 0.8273928278950109 1105 21 0.8684572599488263 1105 22 0.9805285230650114 1105 23 0.6591349548927283 1105 24 0.6393444322882686 1105 25 0.8041374871073117 1106 1 0.3141818368635505 1106 2 2.9932923491319094 1106 3 1.564976200439748 1106 4 0.7119574680594738 1106 5 0.43466973308032664 1106 6 0.26970567705624404 1106 7 0.12779121292275164 1106 8 0.08695397187457132 1106 9 0.00681958035784902 1106 10 -0.011879659068618764 1106 11 0.13611249809538606 1106 12 0.21136177992181615 1106 13 0.08258459250104977 1106 14 0.14167893089366856 1106 15 -0.07259256167935746 1106 16 0.09956779981600761 1106 17 0.05961582759812245 1106 18 -0.00914858361007731 1106 19 -0.34698776242539964 1106 20 -0.06850717461529143 1106 21 -0.31550217827800964 1106 22 -0.2360273991837616 1106 23 -0.043962301964398875 1106 24 0.04015809290814332 1106 25 -0.27670082437881405 1107 1 0.9114891704926862 1107 2 3.6704163993539183 1107 3 2.215266551561097 1107 4 1.33273009705551 1107 5 0.8911294323669168 1107 6 0.768373948624621 1107 7 0.6295869306310635 1107 8 0.5378539985133131 1107 9 0.5197891158974954 1107 10 0.7065074758524901 1107 11 0.8108084906259734 1107 12 0.6571010896519596 1107 13 0.592412368964105 1107 14 0.88815735789359 1107 15 0.594456153859646 1107 16 0.7144255729982021 1107 17 0.49686228538948285 1107 18 0.6420914239104718 1107 19 0.7668777629490009 1107 20 0.94993316830044 1107 21 0.8438329066067354 1107 22 0.7855776312993186 1107 23 0.8253294591896534 1107 24 0.8826176835275845 1107 25 0.9414759937497621 1108 1 0.8263151433347744 1108 2 3.222041224687228 1108 3 1.9890445699259054 1108 4 1.179212095409973 1108 5 0.8401551817777602 1108 6 0.5955803068221712 1108 7 0.6213359120698243 1108 8 0.5723732888729316 1108 9 0.7075917847893365 1108 10 0.4302666127033632 1108 11 0.6470265842935135 1108 12 0.6557829366497714 1108 13 0.5491243411128579 1108 14 0.6677480401710056 1108 15 0.6284332351531887 1108 16 0.5147237435723824 1108 17 0.5374046264231254 1108 18 0.4985966130276154 1108 19 0.5438669076011919 1108 20 0.5063817890109235 1108 21 0.5018301921353228 1108 22 0.5098406558640676 1108 23 0.525703308089831 1108 24 0.7306731413763785 1108 25 0.696881969139838 1109 1 0.690207273390012 1109 2 3.3041949699040494 1109 3 1.8262574811611643 1109 4 0.9280786693596355 1109 5 0.5452615902511511 1109 6 0.4262691430666621 1109 7 0.23614195281815853 1109 8 0.14212466194885565 1109 9 0.3041854171742884 1109 10 0.22606773080753584 1109 11 0.3099762224614864 1109 12 0.36795361450533903 1109 13 0.2589849513164424 1109 14 0.48300745133947487 1109 15 0.31621069863604845 1109 16 0.3521841620560622 1109 17 0.3813763516665034 1109 18 0.4675428018088539 1109 19 0.32794482524020907 1109 20 0.3699664993488696 1109 21 0.23928049425131093 1109 22 0.37048032459722435 1109 23 0.4928855166402215 1109 24 0.4948846736426802 1109 25 0.3410122168828096 1110 1 0.5876904824469911 1110 2 3.306889896779687 1110 3 1.804711701492618 1110 4 0.9517729884888482 1110 5 0.36390333541839937 1110 6 0.09206266121724127 1110 7 0.14559163063464098 1110 8 0.16920784918929854 1110 9 0.16929785026374589 1110 10 -0.06052512973023738 1110 11 0.01802564860860656 1110 12 -0.1990328981989009 1110 13 -0.050858592936066316 1110 14 -0.06248782304245282 1110 15 -0.14843092576107608 1110 16 -0.07126511059612284 1110 17 0.0014778570076643405 1110 18 0.06389447272195525 1110 19 0.0939083955167348 1110 20 -0.08714403648090041 1110 21 -0.1362907289552524 1110 22 -0.05429936732208773 1110 23 -0.07868587798199655 1110 24 0.036475858981965814 1110 25 0.03919258121899816 1111 1 -0.717356743787267 1111 2 1.6852119881350305 1111 3 0.33683025596781846 1111 4 -0.388254657346657 1111 5 -0.8884291888895852 1111 6 -1.0291377735718399 1111 7 -0.9391507965406731 1111 8 -1.3975930759783388 1111 9 -1.4315523708533107 1111 10 -1.3460666555619696 1111 11 -1.4294393225791708 1111 12 -1.5709845353584768 1111 13 -1.3360248898334817 1111 14 -1.7395953837069413 1111 15 -1.7925101506850112 1111 16 -1.5440404149733027 1111 17 -1.5955240340270436 1111 18 -1.7433269470927115 1111 19 -1.9249297348902061 1111 20 -1.6003517475347664 1111 21 -1.439058786847717 1111 22 -1.497626397206389 1111 23 -1.7606371677073795 1111 24 -1.929253231683468 1111 25 -1.8122430665224272 1112 1 0.13854685127491706 1112 2 2.99108399621349 1112 3 1.4761171462719567 1112 4 0.5113194671816714 1112 5 0.10991926310429534 1112 6 -0.06938081041031552 1112 7 -0.15742465934827976 1112 8 -0.1894014368943857 1112 9 -0.3426880631692555 1112 10 -0.3017946318729514 1112 11 -0.2586469161388436 1112 12 -0.22683178607131538 1112 13 -0.12930235038633953 1112 14 -0.29783080552122954 1112 15 -0.3316626438614918 1112 16 -0.1980587915242964 1112 17 -0.25224596368686036 1112 18 -0.14056412265536267 1112 19 -0.20818351130314738 1112 20 -0.1863754219180862 1112 21 -0.04374439269582098 1112 22 -0.15954712973171326 1112 23 -0.23453095251098688 1112 24 -0.4356062400082834 1112 25 -0.295846129118317 1113 1 0.3423919642124602 1113 2 2.790511593790505 1113 3 1.4733520481358227 1113 4 0.4885930262018334 1113 5 0.24293539329523384 1113 6 0.20974703607071632 1113 7 0.018383623566442964 1113 8 -0.006797654222062022 1113 9 -0.02687771094906566 1113 10 -0.0022789306788553176 1113 11 -0.08565840078419029 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19 -2.160243083101804 2315 20 -2.087779216018994 2315 21 -2.0800323075666967 2315 22 -1.9521520018870078 2315 23 -2.1053298512226704 2315 24 -1.9769473024959439 2315 25 -1.834683503219452 2316 1 -1.3447264858424468 2316 2 -0.05794093257417643 2316 3 -0.32273490528450566 2316 4 -0.9642211859081513 2316 5 -0.9833556926238636 2316 6 -1.1563346765123725 2316 7 -1.301265278102612 2316 8 -1.2113497556551351 2316 9 -1.1692707133193991 2316 10 -1.27883631742705 2316 11 -1.9172166749920123 2316 12 -1.322738184121306 2316 13 -1.4152271115976163 2316 14 -1.5607198120126924 2316 15 -1.9055131793337168 2316 16 -1.518264507794477 2316 17 -1.6786641786863612 2316 18 -1.9947763718306817 2316 19 -1.7823496976946842 2316 20 -1.9244366403088264 2316 21 -1.871352744727524 2316 22 -1.6615492334894668 2316 23 -1.5107872409884422 2316 24 -1.465107494104437 2316 25 -1.2974348545124883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114611.0 3 7.0 4 1045.0 5 5022.0 6 6042.0 7 5414.0 8 5029.0 9 4874.0 10 4825.0 11 4634.0 12 4525.0 13 4659.0 14 4714.0 15 4868.0 16 5094.0 17 5700.0 18 6220.0 19 7612.0 20 10130.0 21 13117.0 22 16645.0 23 19855.0 24 22914.0 25 26438.0 26 29908.0 27 33630.0 28 39193.0 29 47039.0 30 58338.0 31 73604.0 32 93037.0 33 121265.0 34 160415.0 35 218437.0 36 308902.0 37 385465.0 38 836625.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.531732360291265 19.134513619193964 13.408555153565583 24.92519886694919 2 15.190187406830697 20.57994416443087 41.991740688670696 22.238127740067743 3 19.30769650888683 23.985221333120776 29.459985268568172 27.24709688942422 4 11.877327617892048 16.855016436321986 38.922642269762335 32.34501367602364 5 12.77435077635236 38.10495923762626 36.22651716772724 12.894172818294136 6 31.39804571992441 37.50359337630501 18.420191250649946 12.678169653120639 7 28.10317059632838 31.440541354448992 21.89288055127456 18.563407497948074 8 24.04837018714313 39.10358909032268 20.417727764367857 16.430312958166336 9 25.827609773522685 16.11552955659571 20.69836286262128 37.35849780726032 10 14.551327637089774 29.81228081376103 34.801691232993285 20.83470031615591 11 34.02812400245776 22.533558935487502 25.199895932703146 18.23842112935159 12 23.421274434985925 24.557170080639327 32.44046520759129 19.58109027678346 13 29.087529503456278 22.34516866603785 26.002748489585407 22.56455334092046 14 20.01569094014268 23.262483006401844 27.914780334218502 28.807045719236974 15 22.2706258496774 32.631708299936676 23.380243644302347 21.71742220608358 16 21.017863706209784 28.731753616064644 29.499433917424273 20.7509487603013 17 20.728247488420017 29.520733266220102 28.83268729463793 20.91833195072196 18 20.390797538766787 28.075323597841106 31.670404083946472 19.86347477944563 19 23.020698903997662 27.215872124327017 27.845559583386997 21.91786938828832 20 22.768734233456293 28.257742489257716 29.087086674844237 19.886436602441755 21 22.043983377988383 27.534782961699992 27.557700966172877 22.863532694138748 22 22.075631216540515 29.39773939620054 28.064028176176386 20.46260121108256 23 21.498395256587347 28.71012728344267 28.461287802437024 21.33018965753296 24 22.538543243648217 27.33132463866747 29.57889010516122 20.551242012523094 25 22.077111122920765 28.18450957467538 28.986421852580115 20.751957449823742 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 243.0 1 243.0 2 373.5 3 504.0 4 504.0 5 504.0 6 1247.5 7 1991.0 8 1991.0 9 1991.0 10 3174.0 11 4357.0 12 4357.0 13 4357.0 14 7809.0 15 11261.0 16 11261.0 17 11261.0 18 20913.0 19 30565.0 20 30565.0 21 30565.0 22 51227.5 23 71890.0 24 71890.0 25 71890.0 26 106403.0 27 140916.0 28 140916.0 29 140916.0 30 188388.5 31 235861.0 32 235861.0 33 235861.0 34 264227.0 35 292593.0 36 292593.0 37 292593.0 38 310347.5 39 328102.0 40 328102.0 41 328102.0 42 344394.5 43 360687.0 44 360687.0 45 360687.0 46 360379.0 47 360071.0 48 360071.0 49 360071.0 50 337935.5 51 315800.0 52 315800.0 53 315800.0 54 272369.0 55 228938.0 56 228938.0 57 228938.0 58 191886.5 59 154835.0 60 154835.0 61 154835.0 62 123118.0 63 91401.0 64 91401.0 65 91401.0 66 69330.0 67 47259.0 68 47259.0 69 47259.0 70 34068.5 71 20878.0 72 20878.0 73 20878.0 74 14375.5 75 7873.0 76 7873.0 77 7873.0 78 5307.5 79 2742.0 80 2742.0 81 2742.0 82 1746.5 83 751.0 84 751.0 85 751.0 86 496.5 87 242.0 88 242.0 89 242.0 90 153.5 91 65.0 92 65.0 93 65.0 94 43.5 95 22.0 96 22.0 97 22.0 98 13.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.76315459294456 3 0.0 4 0.0 5 0.0 6 1.8451192168428385E-4 7 0.007528086404718782 8 3.6902384336856774E-5 9 0.0 10 0.004169969430064815 11 0.0024724597505694037 12 2.952190746948542E-4 13 0.0 14 2.952190746948542E-4 15 0.0 16 0.0 17 1.476095373474271E-4 18 0.011661153450446741 19 0.0035057265120013933 20 0.0 21 0.0070852577926765 22 0.0016606072951585549 23 0.0026938740565905445 24 0.010996910532383318 25 0.007528086404718782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2709852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.03609813165806 #Duplication Level Percentage of deduplicated Percentage of total 1 87.37439659754746 65.56233797287982 2 9.124900888182792 13.693939169746756 3 1.903537843671722 4.285021573052283 4 0.6320044626027194 1.896925955020139 5 0.280993328528112 1.0542321486888322 6 0.15922857208100163 0.7168734456020297 7 0.10175570883404218 0.5344745947469329 8 0.0669472412736559 0.4018767812683066 9 0.04631535096931172 0.31277908983019126 >10 0.2684651021652841 3.5492021889324836 >50 0.021240259320557692 1.1205789379799782 >100 0.016371718979828826 2.519534718643231 >500 0.0018965088578791957 0.996034026020157 >1k 0.001796692602086519 2.5948864042438147 >5k 1.497243835072099E-4 0.7613029933450594 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 8096 0.2987617035911924 No Hit CTGTAGGACGTGGAATATGGCAAGA 6718 0.24791021797500382 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5515 0.2035166496177651 No Hit CTTTAGGACGTGAAATATGGCGAGG 4963 0.18314653346382018 No Hit GTATCAACGCAGAGTACTTTTTTTT 4911 0.1812276094783036 No Hit GTCCTACAGTGTGCATTTCTCATTT 3899 0.14388239652940454 No Hit GGTATCAACGCAGAGTACTTTTTTT 3022 0.11151900546598117 No Hit TATCAACGCAGAGTACTTTTTTTTT 2868 0.10583603827810523 No Hit CTGTAGGACCTGGAATATGGCGAGA 2800 0.10332667614319896 No Hit CTGAAGGACCTGGAATATGGCGAGA 2785 0.10277314037814612 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.6902384336856774E-5 0.0 9 0.0 0.0 0.0 1.1070715301057031E-4 0.0 10 0.0 0.0 0.0 1.1070715301057031E-4 0.0 11 0.0 0.0 0.0 1.476095373474271E-4 0.0 12 0.0 0.0 0.0 1.476095373474271E-4 0.0 13 0.0 0.0 0.0 1.476095373474271E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1160 0.0 13.23964 4 TCCAACG 430 0.0 12.491571 18 CCAACGA 280 0.0 12.115885 19 GGCGAGG 1455 0.0 11.722662 19 GTCCTAC 4075 0.0 11.330127 1 TGTAGGA 3660 0.0 11.191472 2 AGGACGT 3500 0.0 11.037288 5 TCCTACA 4410 0.0 11.002187 2 GTACCGT 60 0.006249035 10.99242 6 GGACGTG 3440 0.0 10.955901 6 TTAGGAC 2025 0.0 10.934259 3 CCCGTTT 355 0.0 10.882636 12 ACTATCG 130 3.6947313E-8 10.870822 11 CTGTAGG 3550 0.0 10.761362 1 AGGACCT 2415 0.0 10.690057 5 GACGTGA 1760 0.0 10.653369 7 CCGTTTC 320 0.0 10.600616 13 GTAGGAC 3695 0.0 10.556804 3 CCTACAG 4640 0.0 10.498303 3 ATTTCTA 3020 0.0 10.483999 15 >>END_MODULE