FastQCFastQC Report
Thu 26 May 2016
SRR1512235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709852
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT87240.32193640095473847No Hit
CTGTAGGACGTGGAATATGGCAAGA81850.30204601579717266No Hit
GTATCAACGCAGAGTACTTTTTTTT66750.24632341544851896No Hit
GTCCTAAAGTGTGTATTTCTCATTT60970.22499383730181574No Hit
CTTTAGGACGTGAAATATGGCGAGG57920.21373861007907444No Hit
GGTATCAACGCAGAGTACTTTTTTT41730.15399364983770333No Hit
TATCAACGCAGAGTACTTTTTTTTT41520.15321869976662933No Hit
GTCCTACAGTGTGCATTTCTCATTT41020.15137358054978647No Hit
CTGAAGGACCTGGAATATGGCGAGA32120.11853045848998396No Hit
CTGTAGGACCTGGAATATGGCGAGA32100.11845665372131023No Hit
TTGTAGAACAGTGTATATCAATGAG30630.11303200322379231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14400.012.7989474
TACACCG853.9452116E-612.294445
TCCAACG4200.012.21054818
CCAACGA2700.011.96062819
TACGGTG802.8674329E-511.8753125
ATGTCGA1850.011.80719817
TATGTCG2100.011.758306516
TGTAGGA45250.011.5262692
CTGTAGG44650.011.3867491
AGGACGT40850.011.2561095
GGACGTG40850.011.20587356
GTAGGAC44450.011.1993943
GGCGAGG16200.011.02253919
GACGTGA20100.011.00906857
TCTAGAC3050.010.9019253
CTACGGT700.001491695510.8574284
GGACCGT700.001495834910.8538226
TGTCGAG2050.010.65527618
GACGTGG21750.010.6542117
TGTAGAA13950.010.6239352
Produced by FastQC (version 0.11.5)