FastQCFastQC Report
Thu 26 May 2016
SRR1512234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890960
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66810.7498653138188022No Hit
GTCCTAAAGTGTGTATTTCTCATTT54820.6152913711053246No Hit
CTGTAGGACGTGGAATATGGCAAGA54480.6114752626380534No Hit
CTTTAGGACGTGAAATATGGCGAGG42630.4784726587052169No Hit
GTCCTACAGTGTGCATTTCTCATTT25170.28250426506240456No Hit
GTATCAACGCAGAGTACTTTTTTTT24600.27610667145550866No Hit
CTGAAGGACCTGGAATATGGCGAGA21880.2455778037173386No Hit
GTCCTTCAGTGTGCATTTCTCATTT19090.214263266588848No Hit
CTGTAGGACCTGGAATATGGCGAGA19010.21336535871419593No Hit
TTTCTAAATTTTCCACCTTTTTCAG18450.20708000359163148No Hit
ATTTAGAAATGTCCACTGTAGGACG16330.18328544491335189No Hit
GAATATGGCAAGAAAACTGAAAATC16040.18003052886773818No Hit
ATTCCAGGTCCTTCAGTGTGCATTT15050.16891891891891891No Hit
TATCAACGCAGAGTACTTTTTTTTT14680.16476609499865313No Hit
GGTATCAACGCAGAGTACTTTTTTT14150.15881745532908323No Hit
TTCCAGGTCCTTCAGTGTGCATTTC13510.15163419233186676No Hit
GGAATATGGCGAGAAAACTGAAAAT13410.15051180748855167No Hit
GCCATATTCCACGTCCTACAGTGGA12740.14299182903834065No Hit
ACCTGGAATATGGCGAGAAAACTGA11720.1315435036365269No Hit
CCATATTTCACGTCCTAAAGTGTGT11670.13098231121486936No Hit
GATATACACTGTTCTACAAATCCCG11630.13053335727754334No Hit
GACCTGGAATATGGCGAGAAAACTG11570.12985992637155427No Hit
ACAGTGGACATTTCTAAATTTTCCA11570.12985992637155427No Hit
CCATATTCCAGGTCCTTCAGTGTGC9620.1079734219269103No Hit
ATTCCACGTCCTACAGTGGACATTT9610.10786118344257879No Hit
CTACAGTGGACATTTCTAAATTTTC9610.10786118344257879No Hit
GTGTATATCAATGAGTTACAATGAA9440.10595312920894318No Hit
TTGTAGAACAGTGTATATCAATGAG9400.10550417527161714No Hit
GTCCACTGTAGGACGTGGAATATGG9400.10550417527161714No Hit
GAAATATGGCGAGGAAAACTGAAAA9280.10415731345963904No Hit
CTGTAGGACATGGAATATGGCAAGA9250.10382059800664453No Hit
TCCTAAAGTGTGTATTTCTCATTTT8920.10011672802370476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3000.014.15028318
GGTATCA5000.013.9457331
AAGACGT552.0788572E-413.7214875
AGACGTG552.0788572E-413.7214876
CAGTAGG602.4250116E-413.4741371
CACCTTT6750.013.41656514
TAGGACC6850.013.3584184
ACACCGT500.001580089913.2069316
CCAACGA1650.013.14975819
ACCTTTT7050.013.11326515
CCACCTT6850.013.08298713
TAGAAAT5800.013.0117554
CCAACGT1600.012.97109319
CCGGTTT2300.012.71474712
GCCGGTT2300.012.71403411
GGCGAGG11550.012.65970519
TGTAGAA4750.012.5522232
GAAATGT6000.012.2635786
CGGTTTC2400.012.18496613
AAATGTC6050.012.1622277
Produced by FastQC (version 0.11.5)