##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512234_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 890960 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.7779047319745 31.0 30.0 34.0 25.0 34.0 2 27.102351396246746 31.0 28.0 34.0 7.0 34.0 3 28.689762727844123 31.0 28.0 34.0 16.0 34.0 4 33.17884304570351 35.0 33.0 37.0 28.0 37.0 5 33.55760752446799 35.0 35.0 37.0 28.0 37.0 6 33.771548666606805 37.0 35.0 37.0 30.0 37.0 7 33.778360420220885 37.0 35.0 37.0 30.0 37.0 8 33.770455463769416 37.0 35.0 37.0 30.0 37.0 9 35.33530686001616 39.0 35.0 39.0 30.0 39.0 10 35.17215250965251 39.0 35.0 39.0 29.0 39.0 11 35.185213702074165 39.0 35.0 39.0 30.0 39.0 12 35.100425383855615 39.0 35.0 39.0 29.0 39.0 13 35.12353416539463 39.0 35.0 39.0 29.0 39.0 14 36.21865628086558 40.0 36.0 41.0 29.0 41.0 15 36.19580564784053 40.0 36.0 41.0 29.0 41.0 16 36.15211120589028 40.0 36.0 41.0 29.0 41.0 17 36.160625617311666 40.0 36.0 41.0 29.0 41.0 18 36.12492592260034 40.0 36.0 41.0 29.0 41.0 19 36.147444329711774 40.0 37.0 41.0 29.0 41.0 20 36.097188425967495 40.0 36.0 41.0 28.0 41.0 21 36.00169480111341 40.0 36.0 41.0 28.0 41.0 22 35.932979033851126 40.0 36.0 41.0 27.0 41.0 23 35.81624203106761 40.0 36.0 41.0 27.0 41.0 24 35.756623192960404 40.0 36.0 41.0 27.0 41.0 25 35.635559396605906 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.10130316837406639 1101 2 3.159646850757362 1101 3 1.6819378531766844 1101 4 0.5445614905408789 1101 5 0.21206486615760411 1101 6 0.13859788456947797 1101 7 -0.17780099305517894 1101 8 -0.17439021179447423 1101 9 -0.01651743085248114 1101 10 -0.03639980011635657 1101 11 0.12957019999522856 1101 12 -0.0837852241044601 1101 13 -0.21139154363931567 1101 14 0.22856342817946285 1101 15 0.04833722803567042 1101 16 0.36358304938294594 1101 17 0.08669274839333951 1101 18 0.2773800294042772 1101 19 0.17433664685151484 1101 20 0.3000438624369366 1101 21 0.2549591052934659 1101 22 0.1381765548029108 1101 23 0.17444455329774655 1101 24 0.15660365806596133 1101 25 0.04223982034114471 1102 1 1.551141995406219 1102 2 4.295013791839086 1102 3 2.7615203795656527 1102 4 1.8999570606052458 1102 5 1.4021151072153657 1102 6 1.343851065387632 1102 7 1.211674077491729 1102 8 1.0579072100857516 1102 9 1.187709122420955 1102 10 1.30925090470501 1102 11 1.2866819976837505 1102 12 1.3948283213337902 1102 13 1.3502190633339808 1102 14 1.72214889519244 1102 15 1.5720522163660107 1102 16 1.29763815261785 1102 17 1.435355325822215 1102 18 1.549314342513803 1102 19 1.3020799878062945 1102 20 1.493932891663981 1102 21 1.7104114583324375 1102 22 1.6782841185973965 1102 23 1.7171483728703834 1102 24 1.3480281334711606 1102 25 1.6211465645608314 1103 1 1.2785561213191734 1103 2 4.237956409826467 1103 3 2.671268697935375 1103 4 1.7779276492906106 1103 5 1.3191430210756678 1103 6 1.2391768218206707 1103 7 0.9637502924518415 1103 8 1.0120381072685731 1103 9 1.0788015645394324 1103 10 1.1473706267250208 1103 11 1.1289945918211615 1103 12 1.0128943973257734 1103 13 1.0987549464931945 1103 14 1.3149626446650515 1103 15 1.224101761336641 1103 16 1.2016535162806434 1103 17 1.1446262076588596 1103 18 1.2185015417932803 1103 19 1.4636334491118532 1103 20 1.4253975768815934 1103 21 1.6141579360356673 1103 22 1.6795585943244262 1103 23 1.677166342322316 1103 24 1.7825882863208022 1103 25 1.7182089308009978 1104 1 1.5539747461486613 1104 2 4.479781991956273 1104 3 2.895456131932221 1104 4 1.861772293519067 1104 5 1.5709595275986885 1104 6 1.319403412794486 1104 7 1.2025063470118624 1104 8 1.2410847031403378 1104 9 1.4123107215939683 1104 10 1.471665660406714 1104 11 1.286445842293503 1104 12 1.414928264077247 1104 13 1.411431208850253 1104 14 1.7196815169405681 1104 15 1.5843579172908022 1104 16 1.57003782556491 1104 17 1.5270705045985267 1104 18 1.5370387179830516 1104 19 1.7275662785403085 1104 20 1.6002941603976737 1104 21 1.7928753610898838 1104 22 1.9845776597474725 1104 23 1.8511453919655523 1104 24 1.945974695516533 1104 25 1.9116722776498563 1105 1 1.1307261910914441 1105 2 4.052366388271796 1105 3 2.5790433700345723 1105 4 1.7999342878173863 1105 5 1.3528187193813892 1105 6 1.0223897221950224 1105 7 0.8626610099302212 1105 8 0.9524390618483025 1105 9 1.1614333439599989 1105 10 1.059761617962124 1105 11 1.1606614084043798 1105 12 0.9079528148067126 1105 13 1.0067283968280591 1105 14 1.0666375456368442 1105 15 1.0545372633516337 1105 16 0.9719169585200191 1105 17 0.9331483747636113 1105 18 1.0314745423785041 1105 19 1.0445833869797738 1105 20 1.2534388561250367 1105 21 1.1735865207901526 1105 22 1.1685219602729333 1105 23 0.9046119531545074 1105 24 0.8206271113748542 1105 25 0.9723196980294659 1106 1 0.28725645189402016 1106 2 3.1545994111798237 1106 3 1.57465209819717 1106 4 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0.6296464671566184 1107 16 0.7871493099216167 1107 17 0.5970274112430971 1107 18 0.6992072999099577 1107 19 0.7111270865519828 1107 20 0.658605279113786 1107 21 0.8392401191006584 1107 22 1.0009065291435348 1107 23 1.008128812055972 1107 24 1.122936636473682 1107 25 1.2406745111194795 1108 1 0.6568832099178188 1108 2 3.1545858879198185 1108 3 1.926883436098361 1108 4 0.907427834865274 1108 5 0.7073486466390193 1108 6 0.2747464034823608 1108 7 0.4537480390452231 1108 8 0.28881498466557787 1108 9 0.4725446054895741 1108 10 0.18476613051604573 1108 11 0.21829687527320374 1108 12 0.47485932371449024 1108 13 0.2657997583558327 1108 14 0.36619892539533083 1108 15 0.31714646715661843 1108 16 -0.0031049273665217925 1108 17 0.2622816485312356 1108 18 0.4089530626218263 1108 19 0.48231352722994814 1108 20 0.395893414707011 1108 21 0.3000452038464161 1108 22 0.2360760206689605 1108 23 0.28355254086952897 1108 24 0.41742816189741205 1108 25 -0.024155997355094883 1109 1 0.8786134055968766 1109 2 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3.1779272588346963 2314 3 1.5849495003365845 2314 4 0.49803088758556413 2314 5 0.2184557259535609 2314 6 -0.09730973473282489 2314 7 -0.1898316500642423 2314 8 -0.38840549478005926 2314 9 -0.2521416947258004 2314 10 -0.3438063013595922 2314 11 -0.41301106554521994 2314 12 -0.5529159829621619 2314 13 -0.34689688927035434 2314 14 -0.6236877681152961 2314 15 -0.4223036218030245 2314 16 -0.5729885775210306 2314 17 -0.4469315268597214 2314 18 -0.41770790750646114 2314 19 -0.5683197249472229 2314 20 -0.582632196305255 2314 21 -0.47256107951344717 2314 22 -0.4507520999415888 2314 23 -0.6254382354203827 2314 24 -0.41935664941451023 2314 25 -0.28052363477432607 2315 1 -0.8400040319570188 2315 2 -24.848592078181877 2315 3 -13.461644143184696 2315 4 -6.047212020249987 2315 5 -3.079875265249008 2315 6 -2.470183368757251 2315 7 -1.912001040661071 2315 8 -1.495884271225286 2315 9 -1.6375781331470165 2315 10 -1.554020558198296 2315 11 -1.4469610530324672 2315 12 -1.551387502010428 2315 13 -1.7658482563688693 2315 14 -2.0623528177492148 2315 15 -2.219432747001143 2315 16 -2.1273138192738372 2315 17 -1.939497667990743 2315 18 -1.7718932630932471 2315 19 -2.2536618364583134 2315 20 -2.1556701003228156 2315 21 -2.1310373469911994 2315 22 -1.9423120873646624 2315 23 -1.7665038086649076 2315 24 -1.6812671864362159 2315 25 -1.5646144709470917 2316 1 -0.8214083646338857 2316 2 0.5439199991128092 2316 3 0.31115122452053257 2316 4 -0.4011777493482711 2316 5 -0.6882595167933871 2316 6 -0.7121374497887416 2316 7 -0.812571661274113 2316 8 -0.8678060339211697 2316 9 -0.6763643439904925 2316 10 -0.9473574263495834 2316 11 -1.5369894999007911 2316 12 -0.95623356910464 2316 13 -0.8813231844100713 2316 14 -1.1423575308398242 2316 15 -1.173299164547302 2316 16 -1.2631812804329243 2316 17 -1.0093397794553383 2316 18 -1.6561363912388174 2316 19 -1.5503719308884527 2316 20 -1.6079037686222293 2316 21 -1.5041542148058369 2316 22 -1.5548076688422157 2316 23 -1.2187963905969426 2316 24 -1.2721751296771444 2316 25 -0.9487650205271265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30880.0 3 0.0 4 239.0 5 1343.0 6 1483.0 7 1397.0 8 1277.0 9 1137.0 10 1124.0 11 1042.0 12 1043.0 13 982.0 14 979.0 15 1010.0 16 1073.0 17 1199.0 18 1314.0 19 1661.0 20 2262.0 21 2876.0 22 3513.0 23 4099.0 24 4770.0 25 5452.0 26 6238.0 27 7318.0 28 8560.0 29 10417.0 30 13511.0 31 17641.0 32 23909.0 33 33609.0 34 48467.0 35 72040.0 36 107923.0 37 148018.0 38 321154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.31826344617042 19.761380982311213 14.039463051090959 26.880892520427402 2 12.770766532132505 20.0982496131692 46.01176542724515 21.119218427453145 3 17.752985543683216 24.29637694172578 30.67376762144204 27.276869893148962 4 10.81305558049744 17.069677651073 39.946686719942534 32.170580048487025 5 10.910029630959864 38.61160994881925 38.12741312741313 12.350947292807758 6 28.936348361709125 39.15993889730055 19.11098041548489 12.792732325505437 7 25.836022516177188 31.51546443824607 23.665669564436563 18.982843481140176 8 21.29601777857592 42.51537667235342 21.233837658256263 14.954767890814402 9 24.849824907964443 15.335929783604202 22.234555984555985 37.57968932387537 10 14.427267655968867 29.177231457879454 36.43065512368523 19.964845762466453 11 31.619897658537788 21.333334829881732 28.99020022695156 18.056567284628912 12 24.049196538104532 23.40965950096357 34.58943585380664 17.95170810712526 13 28.47737272155877 23.41575379366077 26.900422016701086 21.206451468079376 14 17.36137066406272 25.409139631070033 28.288072910528587 28.941416794338657 15 19.30838645954925 37.48776600520787 23.355145012121756 19.84870252312113 16 17.615156684924127 28.343359971266946 34.50570171500404 19.535781628804884 17 18.11921971805693 30.987474185148606 31.73105414384484 19.162251952949628 18 17.908797530517752 27.141518170338152 37.08657219026239 17.86311210888172 19 22.345725987758872 26.081930026006507 28.970818166614475 22.60152581962015 20 21.801427673520696 28.774243512615605 31.15089341833528 18.27343539552842 21 19.075349538887043 27.651848492739827 28.728863785346753 24.543938183026373 22 20.643953424159363 32.232578395257114 29.034473934096127 18.088994246487395 23 17.9747926607111 31.013400588822254 31.49267451688178 19.51913223358487 24 22.130765716569144 26.894984908367014 32.81714598082145 18.157103394242395 25 19.52630775516435 29.178845143649905 32.16936276820711 19.125484332978644 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202.0 1 202.0 2 243.5 3 285.0 4 285.0 5 285.0 6 688.5 7 1092.0 8 1092.0 9 1092.0 10 1505.5 11 1919.0 12 1919.0 13 1919.0 14 3140.5 15 4362.0 16 4362.0 17 4362.0 18 8266.0 19 12170.0 20 12170.0 21 12170.0 22 21205.5 23 30241.0 24 30241.0 25 30241.0 26 46428.0 27 62615.0 28 62615.0 29 62615.0 30 84290.5 31 105966.0 32 105966.0 33 105966.0 34 113075.5 35 120185.0 36 120185.0 37 120185.0 38 119631.5 39 119078.0 40 119078.0 41 119078.0 42 118134.5 43 117191.0 44 117191.0 45 117191.0 46 114381.5 47 111572.0 48 111572.0 49 111572.0 50 98737.5 51 85903.0 52 85903.0 53 85903.0 54 68791.0 55 51679.0 56 51679.0 57 51679.0 58 42070.0 59 32461.0 60 32461.0 61 32461.0 62 25506.0 63 18551.0 64 18551.0 65 18551.0 66 13910.0 67 9269.0 68 9269.0 69 9269.0 70 6677.0 71 4085.0 72 4085.0 73 4085.0 74 2764.0 75 1443.0 76 1443.0 77 1443.0 78 967.5 79 492.0 80 492.0 81 492.0 82 311.0 83 130.0 84 130.0 85 130.0 86 91.0 87 52.0 88 52.0 89 52.0 90 31.0 91 10.0 92 10.0 93 10.0 94 7.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.644630510909582 3 0.0 4 0.0 5 0.0 6 1.1223848433150759E-4 7 0.005050731794917842 8 0.0 9 0.0 10 0.00325491604561372 11 0.0023570081709616596 12 3.3671545299452277E-4 13 0.0 14 5.61192421657538E-4 15 0.0 16 0.0 17 0.0 18 0.009540271168178144 19 0.00325491604561372 20 0.0 21 0.008081170871868546 22 0.0029182005926191973 23 0.00325491604561372 24 0.007744455418874024 25 0.004938493310586334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 890960.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.86593308594098 #Duplication Level Percentage of deduplicated Percentage of total 1 90.10028415960906 62.048401399599065 2 6.729000221478004 9.267977579751726 3 1.4193878983741561 2.9324241609728707 4 0.5374089514752663 1.4803667556832558 5 0.29024750927428716 0.9994082776022046 6 0.17029549830449497 0.7036535034644594 7 0.12222610301316356 0.5892050242021895 8 0.07863751583955947 0.43323567230813803 9 0.06864567827667578 0.4254613818156528 >10 0.39696167084416434 5.163790381539634 >50 0.04111404409691704 1.9765158232714817 >100 0.03676629520069281 5.283709313221026 >500 0.005180705196692302 2.699629033234224 >1k 0.0033423904494789044 3.9693995547703627 >5k 5.013585674218357E-4 2.0268221385636664 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6681 0.7498653138188022 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5482 0.6152913711053246 No Hit CTGTAGGACGTGGAATATGGCAAGA 5448 0.6114752626380534 No Hit CTTTAGGACGTGAAATATGGCGAGG 4263 0.4784726587052169 No Hit GTCCTACAGTGTGCATTTCTCATTT 2517 0.28250426506240456 No Hit GTATCAACGCAGAGTACTTTTTTTT 2460 0.27610667145550866 No Hit CTGAAGGACCTGGAATATGGCGAGA 2188 0.2455778037173386 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1909 0.214263266588848 No Hit CTGTAGGACCTGGAATATGGCGAGA 1901 0.21336535871419593 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1845 0.20708000359163148 No Hit ATTTAGAAATGTCCACTGTAGGACG 1633 0.18328544491335189 No Hit GAATATGGCAAGAAAACTGAAAATC 1604 0.18003052886773818 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1505 0.16891891891891891 No Hit TATCAACGCAGAGTACTTTTTTTTT 1468 0.16476609499865313 No Hit GGTATCAACGCAGAGTACTTTTTTT 1415 0.15881745532908323 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1351 0.15163419233186676 No Hit GGAATATGGCGAGAAAACTGAAAAT 1341 0.15051180748855167 No Hit GCCATATTCCACGTCCTACAGTGGA 1274 0.14299182903834065 No Hit ACCTGGAATATGGCGAGAAAACTGA 1172 0.1315435036365269 No Hit CCATATTTCACGTCCTAAAGTGTGT 1167 0.13098231121486936 No Hit GATATACACTGTTCTACAAATCCCG 1163 0.13053335727754334 No Hit GACCTGGAATATGGCGAGAAAACTG 1157 0.12985992637155427 No Hit ACAGTGGACATTTCTAAATTTTCCA 1157 0.12985992637155427 No Hit CCATATTCCAGGTCCTTCAGTGTGC 962 0.1079734219269103 No Hit ATTCCACGTCCTACAGTGGACATTT 961 0.10786118344257879 No Hit CTACAGTGGACATTTCTAAATTTTC 961 0.10786118344257879 No Hit GTGTATATCAATGAGTTACAATGAA 944 0.10595312920894318 No Hit TTGTAGAACAGTGTATATCAATGAG 940 0.10550417527161714 No Hit GTCCACTGTAGGACGTGGAATATGG 940 0.10550417527161714 No Hit GAAATATGGCGAGGAAAACTGAAAA 928 0.10415731345963904 No Hit CTGTAGGACATGGAATATGGCAAGA 925 0.10382059800664453 No Hit TCCTAAAGTGTGTATTTCTCATTTT 892 0.10011672802370476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.3671545299452277E-4 0.0 5 0.0 0.0 0.0 3.3671545299452277E-4 0.0 6 0.0 0.0 0.0 3.3671545299452277E-4 0.0 7 0.0 0.0 0.0 3.3671545299452277E-4 0.0 8 0.0 0.0 0.0 3.3671545299452277E-4 0.0 9 0.0 0.0 0.0 3.3671545299452277E-4 0.0 10 0.0 0.0 0.0 3.3671545299452277E-4 0.0 11 0.0 0.0 0.0 3.3671545299452277E-4 0.0 12 0.0 0.0 0.0 3.3671545299452277E-4 0.0 13 0.0 0.0 0.0 3.3671545299452277E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 300 0.0 14.150283 18 GGTATCA 500 0.0 13.945733 1 AAGACGT 55 2.0788572E-4 13.721487 5 AGACGTG 55 2.0788572E-4 13.721487 6 CAGTAGG 60 2.4250116E-4 13.474137 1 CACCTTT 675 0.0 13.416565 14 TAGGACC 685 0.0 13.358418 4 ACACCGT 50 0.0015800899 13.206931 6 CCAACGA 165 0.0 13.149758 19 ACCTTTT 705 0.0 13.113265 15 CCACCTT 685 0.0 13.082987 13 TAGAAAT 580 0.0 13.011755 4 CCAACGT 160 0.0 12.971093 19 CCGGTTT 230 0.0 12.714747 12 GCCGGTT 230 0.0 12.714034 11 GGCGAGG 1155 0.0 12.659705 19 TGTAGAA 475 0.0 12.552223 2 GAAATGT 600 0.0 12.263578 6 CGGTTTC 240 0.0 12.184966 13 AAATGTC 605 0.0 12.162227 7 >>END_MODULE