FastQCFastQC Report
Thu 26 May 2016
SRR1512234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890960
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA51180.5744365628086558No Hit
GTATCAACGCAGAGTACTTTTTTTT40460.45411690760527973No Hit
CTTTAGGACGTGAAATATGGCGAGG37810.4243737092574302No Hit
GTCCTACAGTGGACATTTCTAAATT35730.40102810451647664No Hit
GTCCTAAAGTGTGTATTTCTCATTT33800.37936607704049563No Hit
TATCAACGCAGAGTACTTTTTTTTT31230.3505207865672982No Hit
GGTATCAACGCAGAGTACTTTTTTT30410.3413172308521146No Hit
CTGAAGGACCTGGAATATGGCGAGA27810.3121352249259226No Hit
TTTCTAAATTTTCCACCTTTTTCAG21820.24490437281134955No Hit
GAATATGGCAAGAAAACTGAAAATC19770.2218954835233905No Hit
ATTTAGAAATGTCCACTGTAGGACG19510.21897728293077132No Hit
CTGTAGGACCTGGAATATGGCGAGA19320.21684475172847267No Hit
GGAATATGGCGAGAAAACTGAAAAT18950.21269192780820687No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16380.18384663733500944No Hit
GTCCTACAGTGTGCATTTCTCATTT16090.1805917212893957No Hit
ACAGTGGACATTTCTAAATTTTCCA15610.17520427404148334No Hit
ACCTGGAATATGGCGAGAAAACTGA14930.1675720571069408No Hit
ATTCCAGGTCCTTCAGTGTGCATTT14250.15993984017239832No Hit
GAAATATGGCGAGGAAAACTGAAAA13970.1567971626111161No Hit
TTGTAGAACAGTGTATATCAATGAG13590.1525321002065188No Hit
CCACTGTAGGACGTGGAATATGGCA12450.13973691299272695No Hit
GCCATATTCCACGTCCTACAGTGGA12420.13940019753973243No Hit
GACCTGGAATATGGCGAGAAAACTG12240.1373799048217653No Hit
TTCCAGGTCCTTCAGTGTGCATTTC11750.1318802190895214No Hit
GTCCTTCAGTGTGCATTTCTCATTT10260.1151566849241268No Hit
ATTCCACGTCCTACAGTGGACATTT10240.11493220795546377No Hit
CTACAGTGGACATTTCTAAATTTTC10140.11380982311214868No Hit
CTGTAGGACATGGAATATGGCAAGA10010.1123507228158391No Hit
CCATATTTCACGTCCTAAAGTGTGT9920.11134057645685552No Hit
GTAGGACGTGGAATATGGCAAGAAA9750.10943252222321989No Hit
ATACACACTTTAGGACGTGAAATAT9610.10786118344257879No Hit
CACTTTAGGACGTGAAATATGGCGA9560.10729999102092125No Hit
TCCTAAAGTGTGTATTTCTCATTTT9300.10438179042830205No Hit
GATATACACTGTTCTACAAATCCCG9250.10382059800664453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAG250.00603792718.99573310
GCGAGCA400.005284629814.2468019
TTGCGAG707.27107E-613.56838218
CACCTTT6300.013.56838114
CCACCTT6300.013.56838113
CTAAAAC500.001499305713.29925253
ACCTTTT6900.012.80147415
TAGGACC7050.012.8007014
GGTATCA9650.012.6436191
TTCCACC6800.012.57070611
TCCAACG1600.012.46595118
ATACTGG1001.4439502E-712.3472276
TCCACCT6950.012.299396512
TATTGCG701.0921799E-412.21154416
ATTGCGA701.0921799E-412.21154417
ATAGCGA550.003071840212.08819517
TGTCGAG1459.822543E-1111.79045718
GCCGGTT1052.7225542E-711.75926511
CCTGAAC658.0213026E-411.6916513
TAGCGAG658.032642E-411.68968218
Produced by FastQC (version 0.11.5)