##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512234_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 890960 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.83165910927539 31.0 28.0 31.0 16.0 34.0 2 28.043961569542965 31.0 28.0 31.0 16.0 34.0 3 28.03126739696507 31.0 27.0 31.0 16.0 34.0 4 31.445874113315973 35.0 32.0 37.0 20.0 37.0 5 30.796324189638142 35.0 30.0 37.0 16.0 37.0 6 30.7857120409446 35.0 31.0 37.0 13.0 37.0 7 30.4957944239921 35.0 30.0 37.0 10.0 37.0 8 30.306338107210202 35.0 30.0 37.0 10.0 37.0 9 31.29855100116728 35.0 30.0 39.0 10.0 39.0 10 30.911305782526714 35.0 30.0 39.0 8.0 39.0 11 30.943284771482446 35.0 30.0 39.0 7.0 39.0 12 30.629541169076052 35.0 30.0 39.0 2.0 39.0 13 30.62757026129119 35.0 30.0 39.0 2.0 39.0 14 31.0040630331328 36.0 29.0 39.0 2.0 40.0 15 30.964726811529136 36.0 29.0 39.0 2.0 40.0 16 30.74652958606447 36.0 29.0 39.0 2.0 40.0 17 30.647126694801113 36.0 29.0 39.0 2.0 40.0 18 30.491119691119692 36.0 28.0 39.0 2.0 40.0 19 30.536676169525006 36.0 28.0 39.0 2.0 40.0 20 30.36079060788363 36.0 27.0 39.0 2.0 40.0 21 30.09669906617581 36.0 27.0 39.0 2.0 40.0 22 30.18366705576008 36.0 27.0 39.0 2.0 40.0 23 29.931326883361766 36.0 27.0 39.0 2.0 40.0 24 29.686038654934002 36.0 27.0 39.0 2.0 40.0 25 29.09707394271348 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24658666426385878 1101 2 -0.15457846377055873 1101 3 0.08019299340737618 1101 4 0.02001400399627329 1101 5 -0.018820797727013172 1101 6 -0.09732289134516847 1101 7 -0.10861538415407779 1101 8 -0.24125780262511043 1101 9 -0.05886105589952351 1101 10 0.020987842593228834 1101 11 -0.1575764775001467 1101 12 -0.29788935476423717 1101 13 -0.556878640860095 1101 14 -0.4563569675947434 1101 15 -0.35417872448227783 1101 16 -0.7151591652042768 1101 17 -0.7761323600634817 1101 18 -0.5537944965576926 1101 19 -1.0655251073517071 1101 20 -0.7584173477845049 1101 21 -1.057029243326518 1101 22 -0.9234885852196477 1101 23 -0.9655610344689372 1101 24 -1.3851671290541283 1101 25 -1.2393483816710003 1102 1 0.797178327628913 1102 2 0.6751924395969091 1102 3 0.7209273289710048 1102 4 0.6946821042711235 1102 5 1.0430143502865548 1102 6 1.011133669679257 1102 7 0.7003941091820174 1102 8 0.9907470681882273 1102 9 1.1969527218523481 1102 10 0.8862124807906824 1102 11 1.2824084523415706 1102 12 1.2126187341108334 1102 13 1.2267458401586282 1102 14 1.144841952017405 1102 15 1.0331815580939825 1102 16 1.2791960025861755 1102 17 1.4228703754509269 1102 18 1.4002900992792284 1102 19 1.4311132541895937 1102 20 1.7092886848128082 1102 21 1.4517256981956557 1102 22 1.5287273541715471 1102 23 1.705614804915129 1102 24 1.572840457842176 1102 25 1.4488418391348858 1103 1 0.6239906299266877 1103 2 0.6059083976506976 1103 3 0.5023929979177382 1103 4 0.7555596343677635 1103 5 0.6956421093734804 1103 6 0.6827550520154162 1103 7 0.6234916317148809 1103 8 0.8059109246687051 1103 9 0.9047993825291982 1103 10 0.7153441977477684 1103 11 0.7923681631689341 1103 12 0.8935046586959743 1103 13 0.8752463090695102 1103 14 1.0139567925549144 1103 15 1.1637934594674881 1103 16 1.2199066485027146 1103 17 0.8419859811110832 1103 18 0.8744923496142434 1103 19 1.2126762030160059 1103 20 0.6685776016833245 1103 21 1.0571967623500953 1103 22 1.0206301704787144 1103 23 1.2490387943520638 1103 24 0.8721640894203162 1103 25 1.2565359124948436 1104 1 0.629299693676451 1104 2 0.7165875832618163 1104 3 0.9932902418809064 1104 4 0.7962846191032398 1104 5 1.1298772041576086 1104 6 1.0208884468131778 1104 7 1.13710892035796 1104 8 0.8985796295607784 1104 9 1.0456829410535633 1104 10 1.3474696295039656 1104 11 1.5942750379133699 1104 12 1.5255613862249824 1104 13 1.5897946508043361 1104 14 1.9518016295984602 1104 15 2.000591000299405 1104 16 1.7761482613233674 1104 17 1.8502637323552378 1104 18 1.9890511077964206 1104 19 2.004599910296104 1104 20 1.8709348215556894 1104 21 1.9619472164074665 1104 22 2.0967289671574214 1104 23 1.7921468184517337 1104 24 2.1790747373565864 1104 25 1.9794428991606843 1105 1 0.3022486218667275 1105 2 0.1792714477750188 1105 3 0.1721768182577499 1105 4 0.34415531610493133 1105 5 0.16483536956974731 1105 6 0.15037252273449297 1105 7 0.5835127755003953 1105 8 0.39838666872673656 1105 9 0.47866215447503535 1105 10 0.4171901701564593 1105 11 0.34294722971078073 1105 12 0.39162959137233955 1105 13 0.4464094726862058 1105 14 0.5011322648753236 1105 15 0.5105293320539701 1105 16 0.6631954399643583 1105 17 0.3485406656700114 1105 18 0.6428035452708833 1105 19 0.9374427496383859 1105 20 0.5560680098005832 1105 21 0.6136636624423772 1105 22 0.8064649446766339 1105 23 0.8957843282270943 1105 24 1.1658605983636718 1105 25 0.7760667070192255 1106 1 -0.4510731554880856 1106 2 -0.592210209941431 1106 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-0.8889379367810406 2315 5 -0.70864928494818 2315 6 -1.2805959996390008 2315 7 -1.108037654380901 2315 8 -0.9077474423633483 2315 9 -1.124236622116566 2315 10 -0.7841174704872671 2315 11 -1.2220693013308406 2315 12 -0.8478163311562597 2315 13 -1.0972521437565206 2315 14 -1.2887806456640973 2315 15 -1.0861874657849349 2315 16 -1.239540438147504 2315 17 -1.4850654020200587 2315 18 -1.5708309461630314 2315 19 -1.7450202456898793 2315 20 -1.817099382302363 2315 21 -1.5706082852519963 2315 22 -1.4506771703319217 2315 23 -1.9109601883280654 2315 24 -1.5344247522762338 2315 25 -1.3943199078464659 2316 1 -0.3564539757162706 2316 2 -0.3347428443334515 2316 3 -0.5458897341955229 2316 4 -0.4640317777815639 2316 5 -0.40013121529543483 2316 6 -0.5762330243322289 2316 7 -0.7683416487251904 2316 8 -0.43956922020845823 2316 9 -0.9228403690236 2316 10 -0.8380150280963861 2316 11 -0.8722948563814228 2316 12 -1.3156831526816184 2316 13 -1.0425550439904931 2316 14 -1.3417818744341474 2316 15 -1.4275330531538515 2316 16 -1.2879127386406957 2316 17 -1.4228820627953631 2316 18 -1.4887810883973707 2316 19 -1.0907170256390444 2316 20 -1.0647975425137801 2316 21 -1.4947696076105608 2316 22 -1.7499163428478681 2316 23 -1.368079480017876 2316 24 -1.7376599523300982 2316 25 -1.2467875474296939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52929.0 3 4.0 4 905.0 5 6105.0 6 7217.0 7 5972.0 8 4814.0 9 4349.0 10 4049.0 11 3826.0 12 3599.0 13 3442.0 14 3270.0 15 3272.0 16 3375.0 17 3560.0 18 3768.0 19 4374.0 20 5351.0 21 6838.0 22 8604.0 23 10360.0 24 12204.0 25 14207.0 26 16034.0 27 18534.0 28 21545.0 29 25185.0 30 30893.0 31 37882.0 32 46313.0 33 56889.0 34 69408.0 35 85487.0 36 104935.0 37 120518.0 38 80943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.49673246784843 24.80677520144713 16.009781195864957 20.686711134839484 2 15.697225464667325 24.538812067881835 41.9364505701715 17.82751189727934 3 21.45158031785939 28.2031741043369 31.02316602316602 19.322079554637696 4 16.052572506060876 21.141240908682768 38.107883631139444 24.698302954116908 5 12.219424214084672 41.20608995265591 35.89686015585267 10.677625677406752 6 30.412813145371288 40.50855257250606 17.628064110622248 11.450570171500404 7 27.03073089700997 33.66267845919009 23.191950255903745 16.114640387896202 8 23.63764927718416 43.194195025590375 20.027610667145552 13.140545030079915 9 26.167504714016342 16.180636616683127 22.26620723713747 35.385651432163066 10 15.931130466014187 32.04621980784771 34.176842955912726 17.845806770225376 11 33.05019305019305 22.23444374607165 28.136055490706653 16.579307713028644 12 24.913913082517734 25.753681422286075 33.235835503277364 16.09656999191883 13 30.83853371644069 23.919480111340576 26.126762144204 19.115224028014726 14 19.570126605010326 27.006936338331688 27.915843584448236 25.507093472209753 15 21.451019125437732 38.374225554458114 22.634461704229146 17.54029361587501 16 19.374382688336176 30.698347849510636 31.841945766364372 18.085323695788812 17 19.907964442848165 33.17814492233097 29.367536140791955 17.54635449402891 18 19.601104426685822 28.661780551315434 35.33783783783784 16.399277184160905 19 24.181781449223312 27.2559935350633 29.530730897009967 19.03149411870342 20 23.81094549699201 29.968236508934183 30.047925832809554 16.172892161264254 21 21.192084942084943 28.33662566220706 28.901297476878874 21.56999191882913 22 22.73457843225285 31.698504983388702 29.225891173565593 16.341025410792852 23 19.886414653856516 31.888861452814943 30.44289305917213 17.781830834156416 24 23.506105773547635 28.875033671545296 31.041011942174734 16.577848612732335 25 21.2764858867279 30.990044887243112 30.738164483291598 16.995304742737392 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 215.0 1 215.0 2 298.0 3 381.0 4 381.0 5 381.0 6 863.0 7 1345.0 8 1345.0 9 1345.0 10 1952.0 11 2559.0 12 2559.0 13 2559.0 14 3988.0 15 5417.0 16 5417.0 17 5417.0 18 9946.5 19 14476.0 20 14476.0 21 14476.0 22 25056.5 23 35637.0 24 35637.0 25 35637.0 26 52952.5 27 70268.0 28 70268.0 29 70268.0 30 90507.5 31 110747.0 32 110747.0 33 110747.0 34 116828.0 35 122909.0 36 122909.0 37 122909.0 38 120876.5 39 118844.0 40 118844.0 41 118844.0 42 117178.0 43 115512.0 44 115512.0 45 115512.0 46 110233.0 47 104954.0 48 104954.0 49 104954.0 50 92902.0 51 80850.0 52 80850.0 53 80850.0 54 63897.0 55 46944.0 56 46944.0 57 46944.0 58 37929.5 59 28915.0 60 28915.0 61 28915.0 62 22710.5 63 16506.0 64 16506.0 65 16506.0 66 12487.0 67 8468.0 68 8468.0 69 8468.0 70 6121.0 71 3774.0 72 3774.0 73 3774.0 74 2617.5 75 1461.0 76 1461.0 77 1461.0 78 998.5 79 536.0 80 536.0 81 536.0 82 351.0 83 166.0 84 166.0 85 166.0 86 110.5 87 55.0 88 55.0 89 55.0 90 34.5 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35175540989494475 2 0.0 3 0.0 4 0.0 5 0.02772290562988237 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006846547544221962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 890960.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.05489247529462 #Duplication Level Percentage of deduplicated Percentage of total 1 91.19014342253774 62.9712554885004 2 5.789906703165161 7.996427696581156 3 1.253931888239337 2.5977039514113174 4 0.5173377263710573 1.42898804271867 5 0.29765746348310423 1.027735206764735 6 0.18487009569933738 0.7659710748249102 7 0.12119024841298573 0.5858145701249082 8 0.09164670047770734 0.506292243776289 9 0.06865438617430836 0.4266829129701795 >10 0.3985340704657143 5.17455937097495 >50 0.038280620549597695 1.8356088489601932 >100 0.038115147496148694 5.572527182356877 >500 0.005190147694866355 2.5904847967926745 >1k 0.004379187117543488 5.946724470824812 >5k 1.621921154645736E-4 0.5732241424179562 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 5118 0.5744365628086558 No Hit GTATCAACGCAGAGTACTTTTTTTT 4046 0.45411690760527973 No Hit CTTTAGGACGTGAAATATGGCGAGG 3781 0.4243737092574302 No Hit GTCCTACAGTGGACATTTCTAAATT 3573 0.40102810451647664 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3380 0.37936607704049563 No Hit TATCAACGCAGAGTACTTTTTTTTT 3123 0.3505207865672982 No Hit GGTATCAACGCAGAGTACTTTTTTT 3041 0.3413172308521146 No Hit CTGAAGGACCTGGAATATGGCGAGA 2781 0.3121352249259226 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2182 0.24490437281134955 No Hit GAATATGGCAAGAAAACTGAAAATC 1977 0.2218954835233905 No Hit ATTTAGAAATGTCCACTGTAGGACG 1951 0.21897728293077132 No Hit CTGTAGGACCTGGAATATGGCGAGA 1932 0.21684475172847267 No Hit GGAATATGGCGAGAAAACTGAAAAT 1895 0.21269192780820687 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1638 0.18384663733500944 No Hit GTCCTACAGTGTGCATTTCTCATTT 1609 0.1805917212893957 No Hit ACAGTGGACATTTCTAAATTTTCCA 1561 0.17520427404148334 No Hit ACCTGGAATATGGCGAGAAAACTGA 1493 0.1675720571069408 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1425 0.15993984017239832 No Hit GAAATATGGCGAGGAAAACTGAAAA 1397 0.1567971626111161 No Hit TTGTAGAACAGTGTATATCAATGAG 1359 0.1525321002065188 No Hit CCACTGTAGGACGTGGAATATGGCA 1245 0.13973691299272695 No Hit GCCATATTCCACGTCCTACAGTGGA 1242 0.13940019753973243 No Hit GACCTGGAATATGGCGAGAAAACTG 1224 0.1373799048217653 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1175 0.1318802190895214 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1026 0.1151566849241268 No Hit ATTCCACGTCCTACAGTGGACATTT 1024 0.11493220795546377 No Hit CTACAGTGGACATTTCTAAATTTTC 1014 0.11380982311214868 No Hit CTGTAGGACATGGAATATGGCAAGA 1001 0.1123507228158391 No Hit CCATATTTCACGTCCTAAAGTGTGT 992 0.11134057645685552 No Hit GTAGGACGTGGAATATGGCAAGAAA 975 0.10943252222321989 No Hit ATACACACTTTAGGACGTGAAATAT 961 0.10786118344257879 No Hit CACTTTAGGACGTGAAATATGGCGA 956 0.10729999102092125 No Hit TCCTAAAGTGTGTATTTCTCATTTT 930 0.10438179042830205 No Hit GATATACACTGTTCTACAAATCCCG 925 0.10382059800664453 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.1223848433150759E-4 0.0 6 0.0 0.0 0.0 1.1223848433150759E-4 0.0 7 0.0 0.0 0.0 1.1223848433150759E-4 0.0 8 0.0 0.0 0.0 1.1223848433150759E-4 0.0 9 0.0 0.0 0.0 1.1223848433150759E-4 0.0 10 0.0 0.0 0.0 1.1223848433150759E-4 0.0 11 0.0 0.0 0.0 1.1223848433150759E-4 0.0 12 0.0 0.0 0.0 1.1223848433150759E-4 0.0 13 0.0 0.0 0.0 1.1223848433150759E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCAG 25 0.006037927 18.995733 10 GCGAGCA 40 0.0052846298 14.246801 9 TTGCGAG 70 7.27107E-6 13.568382 18 CACCTTT 630 0.0 13.568381 14 CCACCTT 630 0.0 13.568381 13 CTAAAAC 50 0.0014993057 13.2992525 3 ACCTTTT 690 0.0 12.801474 15 TAGGACC 705 0.0 12.800701 4 GGTATCA 965 0.0 12.643619 1 TTCCACC 680 0.0 12.570706 11 TCCAACG 160 0.0 12.465951 18 ATACTGG 100 1.4439502E-7 12.347227 6 TCCACCT 695 0.0 12.2993965 12 TATTGCG 70 1.0921799E-4 12.211544 16 ATTGCGA 70 1.0921799E-4 12.211544 17 ATAGCGA 55 0.0030718402 12.088195 17 TGTCGAG 145 9.822543E-11 11.790457 18 GCCGGTT 105 2.7225542E-7 11.759265 11 CCTGAAC 65 8.0213026E-4 11.691651 3 TAGCGAG 65 8.032642E-4 11.689682 18 >>END_MODULE