FastQCFastQC Report
Thu 26 May 2016
SRR1512233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737601
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48970.17887924500319807No Hit
GGTATCAACGCAGAGTACTTTTTTT30860.1127264345680762No Hit
TATCAACGCAGAGTACTTTTTTTTT29570.10801427965580082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1452.7284841E-1112.6651031
TAGGACC4350.012.1309274
TCCAACG2600.011.599606518
GGTATCA17900.010.7154171
GTCCTAC11600.010.6422051
GATATAC7500.010.4745081
TGCGATA901.0327656E-410.47110213
GTAGGAC11750.010.4255923
TAGGACG12750.010.4208754
AGGACGT12300.010.265855
TTAGGAC8550.010.2497213
TACGGGA800.002503315710.2024442
TGTAGGA14100.010.1300862
TCCTACA13900.010.12904552
GGACGTG12450.010.0664786
AGTACCG1901.1641532E-109.91908555
TTTAGGA9900.09.8932792
GTATCAA34550.09.8923851
TGTAGAA8700.09.7333662
ACCGTCC1651.1685188E-89.708688
Produced by FastQC (version 0.11.5)