FastQCFastQC Report
Thu 26 May 2016
SRR1512233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737601
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67620.24700458540159795No Hit
GGTATCAACGCAGAGTACTTTTTTT43820.16006715368674984No Hit
TATCAACGCAGAGTACTTTTTTTTT42230.1542591487948755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT1950.012.66272212
TCCAACG3750.012.40946818
GCGATAC1406.8394E-1011.53212214
TTAGGAC6350.011.5203053
TGCGATA1451.2259989E-911.13446213
TAGGACG11650.010.8460774
AGGACGT11050.010.8331625
GGTATCA20000.010.8258051
CGGTTTC2400.010.68417113
TACCGAT803.7835276E-410.6841717
AGTACCG1701.382432E-1010.6182185
GGACGTG11250.010.467856
GTAGGAC12800.010.4654063
CCTAATA1553.6634447E-910.4199162
GACGCTC1106.0720176E-610.3604098
TGTAGGA13650.010.30092052
GTATACG750.00256203910.1740311
CCAACGA3000.010.13165819
CGATACT1606.21003E-910.09060615
GCTACGC856.5930944E-410.0593653
Produced by FastQC (version 0.11.5)