FastQCFastQC Report
Thu 26 May 2016
SRR1512232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082666
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT77500.25140576371231915No Hit
CTGTAGGACGTGGAATATGGCAAGA67360.21851215798273316No Hit
GTCCTAAAGTGTGTATTTCTCATTT57010.18493732373212018No Hit
GTATCAACGCAGAGTACTTTTTTTT52830.17137763221834607No Hit
CTTTAGGACGTGAAATATGGCGAGG51740.16784173179968248No Hit
GGTATCAACGCAGAGTACTTTTTTT32840.10653116490725884No Hit
TATCAACGCAGAGTACTTTTTTTTT30890.10020547149772308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC400.005578172414.13272310
TACCGAT552.1026279E-413.7051277
TCCAACG6350.013.504118
CGTTAGG954.3244472E-712.9027071
CCAACGA3950.012.40521419
GGCGAGG16250.012.29363919
TAGGACC12500.012.2112674
CGGTTTC4050.011.86566213
TTGGACG658.59987E-411.5966444
CCAACGT3450.011.4717119
TGTAGGA37450.011.4283992
TTAGGAC22700.011.3731413
CCGGTTT4200.011.21753912
AGGACGT39050.011.0509645
GGACGTG38950.011.0067636
CTGTAGG37400.010.8974411
GTAGGAC39500.010.758093
TAGGACG42200.010.7396064
TAGGACT5400.010.643684
GACGTGA19950.010.6266257
Produced by FastQC (version 0.11.5)