FastQCFastQC Report
Thu 26 May 2016
SRR1512232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082666
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT86470.2805039533961837No Hit
CTGTAGGACGTGGAATATGGCAAGA78660.2551687403046584No Hit
GTATCAACGCAGAGTACTTTTTTTT70240.22785472055681671No Hit
GTCCTAAAGTGTGTATTTCTCATTT64350.2087478825146805No Hit
CTTTAGGACGTGAAATATGGCGAGG60820.1972967554707516No Hit
GGTATCAACGCAGAGTACTTTTTTT44250.14354458121638866No Hit
TATCAACGCAGAGTACTTTTTTTTT43430.14088454603904543No Hit
GTCCTACAGTGTGCATTTCTCATTT34030.11039145985974477No Hit
CTGAAGGACCTGGAATATGGCGAGA33440.10847753211019294No Hit
TTGTAGAACAGTGTATATCAATGAG33270.10792606140269494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4300.012.58947618
TAGGACC12200.012.4591454
GGCGAGG17350.011.93381719
TACACCG802.8682025E-511.8751225
CCAACGA2650.011.46902519
GGACGTG37150.011.2996426
AGGACGT38400.011.2318865
GCCGGTT2650.011.11007611
GCGTCCC600.00589021911.0802129
CCTAGAC2500.011.0201133
GGTATCA21300.010.9247481
GACGTGA19850.010.8609197
CGGTTTC3150.010.85408413
TGTAGGA39600.010.8435452
TTAGGAC23050.010.7983743
GACGTGG19100.010.6907047
TAGGACG42550.010.605284
GATATAC13650.010.5498311
GTAGGAC39950.010.5345263
TAGACTG4600.010.5327175
Produced by FastQC (version 0.11.5)