FastQCFastQC Report
Thu 26 May 2016
SRR1512231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796057
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71290.39692504191125333No Hit
GGTATCAACGCAGAGTACTTTTTTT45800.25500304277648206No Hit
TATCAACGCAGAGTACTTTTTTTTT38680.2153606483535879No Hit
GTCCTACAGTGGACATTTCTAAATT21660.12059750887638866No Hit
CTGTAGGACGTGGAATATGGCAAGA18520.10311476751573029No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18360.10222392719162032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGC550.003262681711.99235812
TGTAGGA11800.011.7735342
TAGGACC4250.011.7498414
GTAGGAC11900.011.4014633
GTCCTAC11850.011.2928311
AGGACGT12450.011.20050055
GGTATCA17000.011.1767071
CTGTAGG12350.011.1664911
GTATACG650.006210894311.00106051
TAGGACG12950.010.8408064
GGCGAGG5750.010.816071519
GTACCGT700.001597692510.7680486
GGACGTG12650.010.6510056
AATGCCG1701.6189006E-1010.53169218
GACGTGG6900.010.241357
TTAGGAC7950.010.1923983
GATATAC4250.010.0950911
ATAGGAC1901.1641532E-109.9179393
ACACCGT1054.4939996E-59.8707116
TAGGACA4300.09.8602764
Produced by FastQC (version 0.11.5)