FastQCFastQC Report
Thu 26 May 2016
SRR1512231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796057
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92820.5167987430243026No Hit
GGTATCAACGCAGAGTACTTTTTTT61900.34464385039004886No Hit
TATCAACGCAGAGTACTTTTTTTTT53200.29620440776656864No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26380.1468772984376331No Hit
GTCCTACAGTGGACATTTCTAAATT23560.13117623772519468No Hit
CTGTAGGACGTGGAATATGGCAAGA21000.11692279253943501No Hit
GTGGTATCAACGCAGAGTACATGGG19860.11057555523015139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA551.9584694E-413.8198022
CCCGTTT951.0441927E-611.99606112
GCTCGAG658.0466183E-411.6884711
TCCAACG1354.7530193E-911.25556418
GGTATCA20900.011.13594151
TGTAGGA15300.011.1156832
TATACCG700.00149013910.8584175
TAGGACC4900.010.8584164
GTAGGAC14600.010.8026363
CTGTAGG14750.010.7349361
AATCCCG1701.4006218E-1010.61504919
TAGGACA4950.010.3648524
TGGTATC8600.010.2744612
AGGACGT12700.010.2492335
GACGTGG7150.010.2274117
ACCCGTG750.002655134510.1300087
TATGTCG750.002655134510.13000816
GGACGTG12650.010.0599386
GTGGTAT9050.010.0128661
TGTCGAG1054.120008E-59.94911518
Produced by FastQC (version 0.11.5)