##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512231_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1796057 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.062824843532248 31.0 31.0 34.0 28.0 34.0 2 31.260008451847575 31.0 31.0 34.0 28.0 34.0 3 31.342157292335376 31.0 31.0 34.0 28.0 34.0 4 34.9597924787465 37.0 35.0 37.0 32.0 37.0 5 34.60302540509572 37.0 35.0 37.0 32.0 37.0 6 34.54829106203199 37.0 35.0 37.0 31.0 37.0 7 34.39233777101729 37.0 35.0 37.0 30.0 37.0 8 34.30573584245934 37.0 35.0 37.0 30.0 37.0 9 35.8137681599192 39.0 35.0 39.0 30.0 39.0 10 35.64472786776812 39.0 35.0 39.0 30.0 39.0 11 35.631754448773066 39.0 35.0 39.0 30.0 39.0 12 35.46528534450744 39.0 35.0 39.0 30.0 39.0 13 35.441138003972036 39.0 35.0 39.0 30.0 39.0 14 36.48239560325758 40.0 36.0 41.0 30.0 41.0 15 36.39642617132975 40.0 36.0 41.0 30.0 41.0 16 36.30220533089985 39.0 36.0 41.0 29.0 41.0 17 36.25836429467439 39.0 36.0 41.0 29.0 41.0 18 36.191207740066154 39.0 36.0 41.0 29.0 41.0 19 36.23300262742218 39.0 36.0 41.0 29.0 41.0 20 36.10825046198423 39.0 36.0 41.0 29.0 41.0 21 36.01497224197227 39.0 36.0 41.0 28.0 41.0 22 36.11198865069427 39.0 36.0 41.0 29.0 41.0 23 36.015959961181636 39.0 36.0 41.0 29.0 41.0 24 35.892434928290136 39.0 36.0 41.0 28.0 41.0 25 35.45574054720981 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.010605814952810988 1101 2 0.05099696413623178 1101 3 0.19522181140817807 1101 4 0.11978260183405354 1101 5 0.23021172021437764 1101 6 0.12393274197307846 1101 7 0.06720242501978646 1101 8 0.009335071054451305 1101 9 0.12171761062285924 1101 10 0.15194132225027346 1101 11 0.11178055647798146 1101 12 0.09933751497033683 1101 13 0.003714875186439315 1101 14 0.017518559552378576 1101 15 -0.12313138321071193 1101 16 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1103 3 0.4249542092824896 1103 4 0.41492416606747184 1103 5 0.4037031289116726 1103 6 0.6174584031606827 1103 7 0.37870518939937625 1103 8 0.48544686798300063 1103 9 0.7192846656904663 1103 10 0.6351079686151309 1103 11 0.5620448133498428 1103 12 0.6364391070566313 1103 13 0.606813598353952 1103 14 0.7061005173621808 1103 15 0.6650195095539075 1103 16 0.7767752069123972 1103 17 0.649833705467799 1103 18 0.8214680297862671 1103 19 0.7492117909194036 1103 20 0.6425790901249471 1103 21 0.686850825481919 1103 22 0.7570678486940707 1103 23 0.9382263249069425 1103 24 0.5851148089891751 1103 25 0.7997426798919136 1104 1 0.23822408889085622 1104 2 0.20133331177146374 1104 3 0.17485140144886913 1104 4 0.1829290199455187 1104 5 0.10150708617057802 1104 6 0.3091562492522968 1104 7 0.3367629640311236 1104 8 0.3250076594347817 1104 9 0.5078881645275573 1104 10 0.33803883456045725 1104 11 0.3556521885478716 1104 12 0.45113220039266366 1104 13 0.43433070490331716 1104 14 0.5300378888523909 1104 15 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-0.7097441047623576 2314 14 -0.7436914083659332 2314 15 -0.5651799447061592 2314 16 -0.6348355995537815 2314 17 -0.6298315119108437 2314 18 -0.7054552375476746 2314 19 -0.7435107058553783 2314 20 -0.8064713980997098 2314 21 -0.7596558856049853 2314 22 -0.6235942031599606 2314 23 -0.7445540366093653 2314 24 -0.7348003304890227 2314 25 -0.739741924941697 2315 1 -0.42385530488 2315 2 -0.48830911499902285 2315 3 -0.4972507027409705 2315 4 -0.605731992290302 2315 5 -0.49223712035519185 2315 6 -0.7587421100358043 2315 7 -0.8183897519495957 2315 8 -0.8646081173542441 2315 9 -0.7353890879752285 2315 10 -0.7121152718540813 2315 11 -0.8385508611369588 2315 12 -0.8400454970489406 2315 13 -0.8049166069246354 2315 14 -1.0638499958343033 2315 15 -1.0146450798889077 2315 16 -1.144485790155052 2315 17 -1.24793022415097 2315 18 -1.167314961416075 2315 19 -1.2148644554149115 2315 20 -1.1298824201389834 2315 21 -1.087947561614854 2315 22 -0.8814860369364297 2315 23 -0.9276919302250235 2315 24 -0.8670204549404446 2315 25 -0.7802151646828648 2316 1 -0.667243940226399 2316 2 -0.6066198226623101 2316 3 -0.6865705139561626 2316 4 -0.7437788847740734 2316 5 -0.7832855274334207 2316 6 -0.863534362825213 2316 7 -0.7328985877499719 2316 8 -0.8544285639797735 2316 9 -1.0479780054598038 2316 10 -1.082671895307513 2316 11 -1.2559440202852699 2316 12 -1.2186911139164849 2316 13 -1.1751010757375795 2316 14 -1.4860307121295762 2316 15 -1.495557293354814 2316 16 -1.378037054581668 2316 17 -1.4571980236330546 2316 18 -1.4225461069130532 2316 19 -1.48885818922993 2316 20 -1.3661246873150432 2316 21 -1.2721216480626296 2316 22 -1.477432498619983 2316 23 -1.391310192477647 2316 24 -1.5875043458896414 2316 25 -1.449864230349931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20958.0 3 1.0 4 410.0 5 3553.0 6 4553.0 7 3746.0 8 3103.0 9 2746.0 10 2611.0 11 2508.0 12 2492.0 13 2381.0 14 2370.0 15 2496.0 16 2539.0 17 2601.0 18 2904.0 19 3306.0 20 3858.0 21 5158.0 22 6528.0 23 8473.0 24 10460.0 25 12826.0 26 14692.0 27 17304.0 28 20547.0 29 25003.0 30 32290.0 31 41587.0 32 55097.0 33 71616.0 34 93586.0 35 125205.0 36 180959.0 37 311904.0 38 693686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.12816405876435 19.888305460963675 15.230209200762154 23.753321279509823 2 14.550095013688319 20.33348607533057 41.39679308618825 23.719625824792868 3 18.880135764065393 24.40947030077553 30.37286678540826 26.337527149750816 4 12.996079745798713 17.638749772418137 38.106251639007006 31.258918842776147 5 13.332716954628774 37.59733724399629 35.49099298052336 13.578952820851573 6 31.207194426457512 37.54184861616307 17.880668597934253 13.370288359445162 7 27.78720274467904 32.256492973218556 21.48010892750063 18.476195354601774 8 24.991021999858578 37.256111582204795 19.988396804778468 17.764469613158155 9 25.693226885338273 16.744123376930688 20.653464784246825 36.909184953484214 10 15.448897223195033 29.647388696461192 33.17745483578751 21.72625924455627 11 33.69230486560282 23.530544966000523 23.867672351155893 18.909477817240766 12 23.350428187969534 25.152542486123767 30.832818780250292 20.664210545656402 13 28.20873725054383 22.189997310775773 26.150729069289003 23.450536369391394 14 20.97567059397335 22.822382585853344 27.636595052384195 28.565351767789103 15 22.93696692254199 30.43032598631335 24.07095097761374 22.56175611353092 16 21.890619284354564 29.25458379104895 27.325580424229294 21.529216500367195 17 21.372428603323836 29.061271440717086 27.74054498270378 21.825754973255304 18 21.027673397893274 27.98847698040764 29.927780688474808 21.056068933224278 19 22.702119142098496 27.516108898548318 28.479329998992238 21.302441960360945 20 22.798162864541606 27.488938268662967 28.96361307018653 20.749285796608905 21 22.697497907917178 27.008998044048717 28.26391367311839 22.029590374915717 22 22.522893204391618 27.62902290962926 28.629659303685795 21.218424582293324 23 22.19818190625353 27.50129867815999 28.580885795940773 21.719633619645702 24 22.310149399490108 27.04246023372309 29.165778146239234 21.481612220547568 25 22.35110040786781 27.379261383409663 28.838843492907557 21.43079471581497 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 219.0 1 219.0 2 390.0 3 561.0 4 561.0 5 561.0 6 1379.5 7 2198.0 8 2198.0 9 2198.0 10 3380.0 11 4562.0 12 4562.0 13 4562.0 14 7032.0 15 9502.0 16 9502.0 17 9502.0 18 16420.5 19 23339.0 20 23339.0 21 23339.0 22 36292.5 23 49246.0 24 49246.0 25 49246.0 26 70321.0 27 91396.0 28 91396.0 29 91396.0 30 116145.0 31 140894.0 32 140894.0 33 140894.0 34 157970.0 35 175046.0 36 175046.0 37 175046.0 38 187696.0 39 200346.0 40 200346.0 41 200346.0 42 213391.0 43 226436.0 44 226436.0 45 226436.0 46 229305.0 47 232174.0 48 232174.0 49 232174.0 50 224504.5 51 216835.0 52 216835.0 53 216835.0 54 192556.5 55 168278.0 56 168278.0 57 168278.0 58 143186.0 59 118094.0 60 118094.0 61 118094.0 62 95199.0 63 72304.0 64 72304.0 65 72304.0 66 55138.0 67 37972.0 68 37972.0 69 37972.0 70 27479.0 71 16986.0 72 16986.0 73 16986.0 74 11747.5 75 6509.0 76 6509.0 77 6509.0 78 4385.0 79 2261.0 80 2261.0 81 2261.0 82 1466.0 83 671.0 84 671.0 85 671.0 86 425.0 87 179.0 88 179.0 89 179.0 90 112.0 91 45.0 92 45.0 93 45.0 94 24.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44352712636625674 2 0.0 3 0.0 4 0.0 5 0.03474277264028926 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0073494326739073435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1796057.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.53487077931614 #Duplication Level Percentage of deduplicated Percentage of total 1 85.22145245523379 63.519699463764844 2 10.531690180512978 15.699563333846548 3 2.3230579459197895 5.194464714359852 4 0.7605423325074584 2.267476979025724 5 0.36142416176313635 1.346935159676901 6 0.20522261684405219 0.9177744736478721 7 0.13300262789122957 0.6939333578227586 8 0.08194021078429563 0.48859224179499205 9 0.06334933189878729 0.4249560840328908 >10 0.28647455998902416 3.6732558718164317 >50 0.016545373691084206 0.860225678054821 >100 0.01216472207408571 1.7157183425957259 >500 0.0021635939480626988 1.0693974170829414 >1k 7.460668786423067E-4 0.9718053799043952 >5k 2.23820063592692E-4 1.1562015025733683 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9282 0.5167987430243026 No Hit GGTATCAACGCAGAGTACTTTTTTT 6190 0.34464385039004886 No Hit TATCAACGCAGAGTACTTTTTTTTT 5320 0.29620440776656864 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2638 0.1468772984376331 No Hit GTCCTACAGTGGACATTTCTAAATT 2356 0.13117623772519468 No Hit CTGTAGGACGTGGAATATGGCAAGA 2100 0.11692279253943501 No Hit GTGGTATCAACGCAGAGTACATGGG 1986 0.11057555523015139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.5677520256873805E-5 0.0 0.0 0.0 6 0.0 5.5677520256873805E-5 0.0 0.0 0.0 7 0.0 1.1135504051374761E-4 0.0 0.0 0.0 8 0.0 1.1135504051374761E-4 0.0 0.0 0.0 9 0.0 1.1135504051374761E-4 0.0 0.0 0.0 10 0.0 1.1135504051374761E-4 0.0 0.0 0.0 11 5.5677520256873805E-5 1.1135504051374761E-4 0.0 0.0 0.0 12 5.5677520256873805E-5 1.1135504051374761E-4 0.0 0.0 0.0 13 5.5677520256873805E-5 1.6703256077062144E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGGA 55 1.9584694E-4 13.819802 2 CCCGTTT 95 1.0441927E-6 11.996061 12 GCTCGAG 65 8.0466183E-4 11.68847 11 TCCAACG 135 4.7530193E-9 11.255564 18 GGTATCA 2090 0.0 11.1359415 1 TGTAGGA 1530 0.0 11.115683 2 TATACCG 70 0.001490139 10.858417 5 TAGGACC 490 0.0 10.858416 4 GTAGGAC 1460 0.0 10.802636 3 CTGTAGG 1475 0.0 10.734936 1 AATCCCG 170 1.4006218E-10 10.615049 19 TAGGACA 495 0.0 10.364852 4 TGGTATC 860 0.0 10.274461 2 AGGACGT 1270 0.0 10.249233 5 GACGTGG 715 0.0 10.227411 7 ACCCGTG 75 0.0026551345 10.130008 7 TATGTCG 75 0.0026551345 10.130008 16 GGACGTG 1265 0.0 10.059938 6 GTGGTAT 905 0.0 10.012866 1 TGTCGAG 105 4.120008E-5 9.949115 18 >>END_MODULE