FastQCFastQC Report
Thu 26 May 2016
SRR1512230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605949
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78800.30238504283852063No Hit
CTGTAGGACGTGGAATATGGCAAGA69270.26581487204853205No Hit
GTATCAACGCAGAGTACTTTTTTTT53330.20464713622561304No Hit
GTCCTAAAGTGTGTATTTCTCATTT50670.1944397223429929No Hit
CTTTAGGACGTGAAATATGGCGAGG45960.17636569249820314No Hit
GTCCTACAGTGTGCATTTCTCATTT36770.1411002287458427No Hit
GGTATCAACGCAGAGTACTTTTTTT34600.1327731279468631No Hit
TATCAACGCAGAGTACTTTTTTTTT31320.1201865424073917No Hit
CTGTAGGACCTGGAATATGGCGAGA26820.10291836102701932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG551.0828866E-414.8180011
TCTACGA551.0828866E-414.8180012
ACCGCTT400.00556529114.1375348
TAGGACC11650.012.4590674
CGTAGGA852.6092695E-511.9851472
CCAACGT2100.011.66952319
CCGGTTT3150.011.669312
TCCAACG4300.011.61733418
GTCTAGG2800.011.278881
TGTAGGA36100.011.2032912
CTGTAGG35100.011.1451641
CGGTTTC3400.011.08847513
TGTAGAA12500.011.0838642
CCAACGA2400.010.99628219
AAGCGTG600.00623351110.9960717
GGCCTAC2350.010.8376341
TGCCGGT3400.010.81105510
GACGTGG19500.010.7302327
GTAGGAC38100.010.7115833
GCCGGTT3350.010.691071511
Produced by FastQC (version 0.11.5)