##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512230_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2605949 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.17838338355816 31.0 30.0 34.0 24.0 34.0 2 26.275059488884857 31.0 26.0 34.0 2.0 34.0 3 27.94364010961074 31.0 27.0 34.0 16.0 34.0 4 32.46286899705251 35.0 32.0 37.0 25.0 37.0 5 32.87801833420378 35.0 33.0 37.0 26.0 37.0 6 33.093925475901486 35.0 35.0 37.0 27.0 37.0 7 33.087820981914845 36.0 35.0 37.0 26.0 37.0 8 33.09302100693452 37.0 35.0 37.0 26.0 37.0 9 34.58022279023879 39.0 35.0 39.0 27.0 39.0 10 34.42906096780866 38.0 35.0 39.0 27.0 39.0 11 34.449096663058256 38.0 35.0 39.0 27.0 39.0 12 34.31977371775119 38.0 35.0 39.0 27.0 39.0 13 34.32641083919908 38.0 35.0 39.0 27.0 39.0 14 35.343823305828316 39.0 35.0 41.0 26.0 41.0 15 35.28333248271551 39.0 35.0 41.0 26.0 41.0 16 35.22044560350184 39.0 35.0 41.0 26.0 41.0 17 35.20175951256145 39.0 35.0 41.0 26.0 41.0 18 35.148647191483796 39.0 35.0 41.0 25.0 41.0 19 35.16906010056221 39.0 35.0 41.0 25.0 41.0 20 35.0745805079071 39.0 35.0 41.0 25.0 41.0 21 34.99425852155971 39.0 35.0 41.0 25.0 41.0 22 34.8829662437753 39.0 34.0 41.0 24.0 41.0 23 34.75990512477412 39.0 34.0 41.0 24.0 41.0 24 34.633639031308746 39.0 34.0 41.0 22.0 41.0 25 34.497071892043934 39.0 34.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6920570170259204 1101 2 3.6517749703225633 1101 3 2.1358781204240316 1101 4 0.9319548695344579 1101 5 0.7074675347557928 1101 6 0.6721961004417523 1101 7 0.47342173357802153 1101 8 0.4376059428028043 1101 9 0.1640119899487189 1101 10 0.20520027162073973 1101 11 0.4397522141850061 1101 12 0.26932447521167546 1101 13 0.240085468655586 1101 14 0.22783103413820527 1101 15 0.42193172585715644 1101 16 0.6338601763530747 1101 17 0.5661502824523126 1101 18 0.4401073730541043 1101 19 0.27490614169703065 1101 20 0.4279400399122437 1101 21 0.4695578094100341 1101 22 0.4895367993347506 1101 23 0.5117154652427374 1101 24 0.36941154297282 1101 25 0.3748979715459697 1102 1 1.0633861863270404 1102 2 3.9569570190008676 1102 3 2.3909148750335234 1102 4 1.5497973005117913 1102 5 0.8818868374719955 1102 6 0.9343832392023828 1102 7 0.9246661533277916 1102 8 0.8062661718245749 1102 9 0.8225920038713994 1102 10 0.8105439000766665 1102 11 0.8719969596489605 1102 12 0.7882165360882922 1102 13 0.7452001392052736 1102 14 0.8863771993327703 1102 15 0.9686784160263358 1102 16 0.7906034177839203 1102 17 0.905411613793035 1102 18 0.9978015721484894 1102 19 0.9228384953332096 1102 20 0.9171870015099017 1102 21 1.075246631027241 1102 22 1.0167610758980175 1102 23 1.0732696966530781 1102 24 1.0336264121442937 1102 25 0.9636282610483917 1103 1 0.8793900685762068 1103 2 3.7176427180114615 1103 3 2.3159781534054567 1103 4 1.296647613941218 1103 5 1.0601180565347406 1103 6 0.8687381741728544 1103 7 0.7227123127382384 1103 8 0.7437835557801975 1103 9 0.7851127369939945 1103 10 0.801004265895422 1103 11 0.6807146633920169 1103 12 0.7152647179933851 1103 13 0.7847914696738485 1103 14 0.8724769961551289 1103 15 1.0395221926243678 1103 16 0.85176044436718 1103 17 0.9301966869967018 1103 18 0.8119268269713729 1103 19 1.0810659831300242 1103 20 1.0980133995666748 1103 21 1.0719585689434297 1103 22 1.1303298585059878 1103 23 1.1602433089299922 1103 24 1.3359987980537227 1103 25 1.2094862000592101 1104 1 1.241068409138304 1104 2 4.060704936639905 1104 3 2.5546791677734753 1104 4 1.6896806339558168 1104 5 1.2972471289391265 1104 6 0.9764211180954661 1104 7 0.9712020526571479 1104 8 0.8708883459573542 1104 9 1.041377118711459 1104 10 1.112146148150991 1104 11 1.131922402220475 1104 12 1.1209595526692908 1104 13 1.2228407600824411 1104 14 1.3442330513482332 1104 15 0.9879025550504608 1104 16 1.2290271211047639 1104 17 1.0766512729349245 1104 18 1.236267300064597 1104 19 1.1842079295650194 1104 20 1.175855027132826 1104 21 1.3895383747037755 1104 22 1.3319766361082088 1104 23 1.292482604966402 1104 24 1.3465442051526324 1104 25 1.2642176318448008 1105 1 1.0352959206052503 1105 2 3.7502500407442305 1105 3 2.4360420780908996 1105 4 1.486747857570478 1105 5 1.042740622892282 1105 6 0.7219874550649266 1105 7 0.47351234176234414 1105 8 0.5687588240814279 1105 9 0.5696247663190874 1105 10 0.8892476837400451 1105 11 0.817531943839775 1105 12 0.6194560728127172 1105 13 0.7259182318353794 1105 14 0.7482271213439589 1105 15 0.865339518161008 1105 16 0.5568469179718178 1105 17 0.6740948327090308 1105 18 0.8586869818979537 1105 19 0.895651101231536 1105 20 0.7517003411998644 1105 21 0.7524399509683732 1105 22 0.7599482960956578 1105 23 0.6158889891614479 1105 24 0.611306425151362 1105 25 0.6840623421889163 1106 1 0.39007560547722875 1106 2 3.066563389452387 1106 3 1.4925388244001034 1106 4 0.8663979258392871 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-1.7365961913614854 2315 15 -1.9756952883191232 2315 16 -1.8017742341426555 2315 17 -1.8742770604492804 2315 18 -1.7574506675082517 2315 19 -2.016752453589284 2315 20 -1.890676965752398 2315 21 -1.8290319003137725 2315 22 -1.6349488336082274 2315 23 -1.7012545092223519 2315 24 -1.5223849995413374 2315 25 -1.5347348540241654 2316 1 -1.7254521585994524 2316 2 -0.30136296340469215 2316 3 -0.7811108861789506 2316 4 -1.5656690122539914 2316 5 -1.6614481362579099 2316 6 -1.5040323053855253 2316 7 -1.7277904034099478 2316 8 -1.7190430233666874 2316 9 -1.7652840682199766 2316 10 -1.7416947661663542 2316 11 -2.3511862592338773 2316 12 -2.026565365223547 2316 13 -1.8701595617562958 2316 14 -2.2949236491183385 2316 15 -2.461019832046894 2316 16 -2.109258755369858 2316 17 -2.2113371146201644 2316 18 -2.5810959136852745 2316 19 -2.3702952014583474 2316 20 -2.5056076082643486 2316 21 -2.300302321955421 2316 22 -2.292536675081962 2316 23 -2.2794715960408993 2316 24 -2.1872265773037896 2316 25 -2.0141034254050325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102043.0 3 4.0 4 867.0 5 4539.0 6 5406.0 7 4911.0 8 4594.0 9 4309.0 10 4437.0 11 4159.0 12 4124.0 13 4098.0 14 4258.0 15 4425.0 16 4491.0 17 4999.0 18 5691.0 19 7056.0 20 9222.0 21 12085.0 22 14900.0 23 18129.0 24 20929.0 25 24302.0 26 27116.0 27 30974.0 28 35827.0 29 43475.0 30 54220.0 31 69339.0 32 88361.0 33 115472.0 34 155901.0 35 212224.0 36 300715.0 37 379420.0 38 818927.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70256248299564 19.023242588400617 13.154094727103255 25.120100201500488 2 15.394167431883279 20.40428322222139 41.75502948468779 22.44651986120754 3 19.54957675687437 23.605296957077826 29.319491670788643 27.525634615259165 4 12.073144946428345 16.54468295427117 38.60935114232858 32.77282095697191 5 12.830258765616673 37.99295381452208 36.18213556750343 12.994651852357816 6 31.791116082649477 37.4141434297347 17.97332637488512 12.821414112730697 7 28.290359372529515 31.34455000529595 21.632580694184124 18.73250992799041 8 24.141569923279388 39.23914090413895 20.155536428379833 16.463752744201823 9 25.82452688061048 16.055341067687817 20.652629809716153 37.46750224198555 10 14.720636475133661 29.74808239301388 34.579477268854866 20.951803862997593 11 34.2128318314089 22.526986095670598 24.924910174263655 18.335271898656842 12 23.472388348650224 24.493322389135315 32.23507615465702 19.799213107557446 13 29.17750884610558 22.19298996258177 25.867044980542598 22.76245621077005 14 20.03693865788264 23.208958603069448 27.61496610438759 29.13913663466033 15 22.19157013433494 32.64503641475716 23.235335764437448 21.928057686470456 16 21.08237728366902 28.835790723456217 29.261317086404993 20.82051490646977 17 20.758749122679777 29.55179057747529 28.670383549627065 21.019076750217867 18 20.480932190380283 28.101416614984064 31.423315812419787 19.994335382215862 19 23.06059012920829 27.19567745129266 27.778975927425392 21.964756492073665 20 22.818827229542865 28.195831921499614 28.99419750731883 19.99114334163869 21 21.93482057640743 27.53567959609496 27.528081048108405 23.00141877938921 22 22.113724921351608 29.34492548069728 28.04606619895913 20.49528339899198 23 21.514309013287285 28.721561855422845 28.443190866791383 21.32093826449849 24 22.587362455175125 27.197992398192266 29.56323377707914 20.651411369553472 25 21.962501156080677 28.147695878442864 29.00762802178382 20.882174943692636 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 297.0 1 297.0 2 396.0 3 495.0 4 495.0 5 495.0 6 1353.0 7 2211.0 8 2211.0 9 2211.0 10 3536.5 11 4862.0 12 4862.0 13 4862.0 14 8306.0 15 11750.0 16 11750.0 17 11750.0 18 21297.5 19 30845.0 20 30845.0 21 30845.0 22 50132.5 23 69420.0 24 69420.0 25 69420.0 26 101296.0 27 133172.0 28 133172.0 29 133172.0 30 177588.0 31 222004.0 32 222004.0 33 222004.0 34 248533.0 35 275062.0 36 275062.0 37 275062.0 38 291965.0 39 308868.0 40 308868.0 41 308868.0 42 323613.5 43 338359.0 44 338359.0 45 338359.0 46 340618.5 47 342878.0 48 342878.0 49 342878.0 50 323248.0 51 303618.0 52 303618.0 53 303618.0 54 264883.0 55 226148.0 56 226148.0 57 226148.0 58 190549.0 59 154950.0 60 154950.0 61 154950.0 62 125194.5 63 95439.0 64 95439.0 65 95439.0 66 72872.0 67 50305.0 68 50305.0 69 50305.0 70 36279.0 71 22253.0 72 22253.0 73 22253.0 74 15437.0 75 8621.0 76 8621.0 77 8621.0 78 5850.0 79 3079.0 80 3079.0 81 3079.0 82 2013.0 83 947.0 84 947.0 85 947.0 86 612.5 87 278.0 88 278.0 89 278.0 90 173.0 91 68.0 92 68.0 93 68.0 94 43.0 95 18.0 96 18.0 97 18.0 98 10.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.47777489122005 3 0.0 4 0.0 5 0.0 6 1.5349494560330996E-4 7 0.007099141234153086 8 0.0 9 0.0 10 0.003722252430880267 11 0.0026861615480579244 12 7.674747280165498E-5 13 0.0 14 2.6861615480579243E-4 15 0.0 16 0.0 17 7.674747280165498E-5 18 0.011550494656649075 19 0.0030315251756653718 20 0.0 21 0.007214262443355568 22 0.001803565610838892 23 0.002839656493661234 24 0.009708555309409355 25 0.006216545296934054 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2605949.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.26743588199783 #Duplication Level Percentage of deduplicated Percentage of total 1 86.49459464951175 64.23731762272006 2 9.704217379699294 14.414146840635725 3 2.084741822651634 4.644852889328985 4 0.6810227331453189 2.0231124867221157 5 0.3011435566630737 1.1182579892875804 6 0.1701789947757957 0.7583254549784549 7 0.11086591185483627 0.5763608900124794 8 0.07370303956940166 0.4378988612423109 9 0.058193810699727296 0.3889714594384004 >10 0.28173081364110286 3.6681290341670363 >50 0.020281188147857707 1.0608733764300513 >100 0.015605369283713522 2.391103929978397 >500 0.0017293535460113597 0.9197477981057948 >1k 0.0017817581988704573 2.3798552114691662 >5k 2.0961861163181853E-4 0.9810461554834707 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7880 0.30238504283852063 No Hit CTGTAGGACGTGGAATATGGCAAGA 6927 0.26581487204853205 No Hit GTATCAACGCAGAGTACTTTTTTTT 5333 0.20464713622561304 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5067 0.1944397223429929 No Hit CTTTAGGACGTGAAATATGGCGAGG 4596 0.17636569249820314 No Hit GTCCTACAGTGTGCATTTCTCATTT 3677 0.1411002287458427 No Hit GGTATCAACGCAGAGTACTTTTTTT 3460 0.1327731279468631 No Hit TATCAACGCAGAGTACTTTTTTTTT 3132 0.1201865424073917 No Hit CTGTAGGACCTGGAATATGGCGAGA 2682 0.10291836102701932 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.837373640082749E-5 0.0 0.0 3.837373640082749E-5 6 0.0 3.837373640082749E-5 0.0 0.0 3.837373640082749E-5 7 0.0 7.674747280165498E-5 0.0 3.837373640082749E-5 3.837373640082749E-5 8 0.0 7.674747280165498E-5 0.0 3.837373640082749E-5 3.837373640082749E-5 9 0.0 7.674747280165498E-5 0.0 3.837373640082749E-5 3.837373640082749E-5 10 0.0 7.674747280165498E-5 0.0 3.837373640082749E-5 3.837373640082749E-5 11 0.0 7.674747280165498E-5 0.0 3.837373640082749E-5 3.837373640082749E-5 12 0.0 7.674747280165498E-5 0.0 3.837373640082749E-5 3.837373640082749E-5 13 0.0 7.674747280165498E-5 0.0 3.837373640082749E-5 3.837373640082749E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 55 1.0828866E-4 14.818001 1 TCTACGA 55 1.0828866E-4 14.818001 2 ACCGCTT 40 0.005565291 14.137534 8 TAGGACC 1165 0.0 12.459067 4 CGTAGGA 85 2.6092695E-5 11.985147 2 CCAACGT 210 0.0 11.669523 19 CCGGTTT 315 0.0 11.6693 12 TCCAACG 430 0.0 11.617334 18 GTCTAGG 280 0.0 11.27888 1 TGTAGGA 3610 0.0 11.203291 2 CTGTAGG 3510 0.0 11.145164 1 CGGTTTC 340 0.0 11.088475 13 TGTAGAA 1250 0.0 11.083864 2 CCAACGA 240 0.0 10.996282 19 AAGCGTG 60 0.006233511 10.996071 7 GGCCTAC 235 0.0 10.837634 1 TGCCGGT 340 0.0 10.811055 10 GACGTGG 1950 0.0 10.730232 7 GTAGGAC 3810 0.0 10.711583 3 GCCGGTT 335 0.0 10.6910715 11 >>END_MODULE