FastQCFastQC Report
Thu 26 May 2016
SRR1512230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605949
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT87120.3343119915240091No Hit
CTGTAGGACGTGGAATATGGCAAGA79250.30411186097655785No Hit
GTATCAACGCAGAGTACTTTTTTTT70930.2721849122910694No Hit
GTCCTAAAGTGTGTATTTCTCATTT54270.20825426744729078No Hit
CTTTAGGACGTGAAATATGGCGAGG53300.20453201501641052No Hit
GGTATCAACGCAGAGTACTTTTTTT47990.18415556098757113No Hit
TATCAACGCAGAGTACTTTTTTTTT45730.17548309656098413No Hit
GTCCTACAGTGTGCATTTCTCATTT40420.1551066425321447No Hit
CTGTAGGACCTGGAATATGGCGAGA31190.11968768383418095No Hit
TTGTAGAACAGTGTATATCAATGAG30350.11646428997651144No Hit
CTGAAGGACCTGGAATATGGCGAGA30130.11562006777569324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTT307.739983E-418.99439611
TAGGACC12700.014.8112044
GACCGTC456.779643E-414.773427
GGACCGT1101.8735591E-1013.8141076
CCAACGA2250.012.24106919
CCGAACT550.003069125312.0910564
GGTATCA18850.011.7905961
TGTAGGA41400.011.7718822
GTAGGAC41050.011.4556813
TCCAACG3350.011.33993918
CTGTAGG41750.011.218021
ACTGTTC10350.011.1030048
ATCCCGT2400.011.08006510
AGGACCT26550.010.9850665
CCGTTTC2400.010.68434813
GGACCTG26050.010.682076
AGCACCG1252.2415225E-710.6401285
CGTTTCC2500.010.63686314
TGTAGAA13000.010.5962832
GACGTGG20450.010.5885647
Produced by FastQC (version 0.11.5)