##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512230_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2605949 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.966413387215177 31.0 31.0 34.0 28.0 34.0 2 31.168236216441688 31.0 31.0 34.0 28.0 34.0 3 31.26253737122254 31.0 31.0 34.0 28.0 34.0 4 34.858152634606434 37.0 35.0 37.0 32.0 37.0 5 34.495698112280785 37.0 35.0 37.0 31.0 37.0 6 34.43747978183763 37.0 35.0 37.0 30.0 37.0 7 34.27236296642797 37.0 35.0 37.0 30.0 37.0 8 34.18278369991124 37.0 35.0 37.0 30.0 37.0 9 35.69216358416838 39.0 35.0 39.0 30.0 39.0 10 35.49914906239531 39.0 35.0 39.0 30.0 39.0 11 35.4900909419179 39.0 35.0 39.0 30.0 39.0 12 35.31377897265065 39.0 35.0 39.0 30.0 39.0 13 35.307122664334564 39.0 35.0 39.0 30.0 39.0 14 36.327248154127346 39.0 36.0 41.0 29.0 41.0 15 36.28080979328452 39.0 36.0 41.0 29.0 41.0 16 36.17773141377671 39.0 36.0 41.0 29.0 41.0 17 36.11845550315835 39.0 36.0 41.0 29.0 41.0 18 36.05176118181898 39.0 36.0 41.0 28.0 41.0 19 36.081135125821724 39.0 36.0 41.0 28.0 41.0 20 35.96698515588755 39.0 36.0 41.0 27.0 41.0 21 35.85613417607175 39.0 36.0 41.0 27.0 41.0 22 35.95116443184421 39.0 36.0 41.0 28.0 41.0 23 35.85357272916699 39.0 36.0 41.0 27.0 41.0 24 35.7420233473487 39.0 36.0 41.0 27.0 41.0 25 35.31313391014176 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2687511536523495 1101 2 0.3056545598180591 1101 3 0.3137518385877023 1101 4 0.39927233782666605 1101 5 0.2759434166599348 1101 6 0.34122944812588685 1101 7 0.38589399341604036 1101 8 0.12629581657025568 1101 9 0.27743813879183676 1101 10 0.42227894209473504 1101 11 0.35582378764834743 1101 12 0.23475689661197663 1101 13 0.2771115026044413 1101 14 0.28822836613510816 1101 15 0.2111949733049201 1101 16 0.44850045783803694 1101 17 0.3890029879479755 1101 18 0.28631977438850953 1101 19 0.3067599817001181 1101 20 0.27272149814933755 1101 21 0.2920102687333923 1101 22 0.09860264950640385 1101 23 0.009612373167477983 1101 24 -0.06251165544004778 1101 25 0.07031993584568852 1102 1 0.3791985955187549 1102 2 0.4023013004410032 1102 3 0.24989238106146772 1102 4 0.3730513886572382 1102 5 0.3592869684772282 1102 6 0.3996581007549054 1102 7 0.34068486575524304 1102 8 0.38470371290373606 1102 9 0.5864789424756012 1102 10 0.37619230212385446 1102 11 0.40530136765588054 1102 12 0.5952458510694711 1102 13 0.48032393849942423 1102 14 0.5509612358047349 1102 15 0.6549735132112957 1102 16 0.8495114490948481 1102 17 0.7539194199088399 1102 18 0.8441500070506152 1102 19 0.7247962445450682 1102 20 0.8214927254531261 1102 21 0.5506206187803286 1102 22 0.6465350031425885 1102 23 0.8137278675824362 1102 24 0.8122442185915872 1102 25 0.7525056056992412 1103 1 0.1670939171114938 1103 2 0.20065050918676874 1103 3 0.17414226821078316 1103 4 0.22010491066598092 1103 5 0.16354896522428675 1103 6 0.22043930284750957 1103 7 0.24947289427004193 1103 8 0.3780137325284656 1103 9 0.501412112285017 1103 10 0.38573007995388764 1103 11 0.30852730385247185 1103 12 0.3149641992757921 1103 13 0.30842272237280355 1103 14 0.3982401497897712 1103 15 0.5701439865839504 1103 16 0.6412743237413281 1103 17 0.2994928421572709 1103 18 0.4768435386257579 1103 19 0.5242247668245312 1103 20 0.46729797502872117 1103 21 0.5943373804251451 1103 22 0.8171689501753221 1103 23 0.6178996765997837 1103 24 0.4108716943839923 1103 25 0.4537877565420132 1104 1 0.32050152703617485 1104 2 0.346164049078979 1104 3 0.32546359252307155 1104 4 0.2572361930224787 1104 5 0.3622963159306707 1104 6 0.3870878559797575 1104 7 0.437417211953381 1104 8 0.29818829452655393 1104 9 0.538007556937977 1104 10 0.6457036997858125 1104 11 0.6419173491988701 1104 12 0.6052692604596785 1104 13 0.7000232090466412 1104 14 0.8891897904303718 1104 15 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2314 15 -0.60277971123611 2314 16 -0.6558820806258794 2314 17 -0.6331817850931145 2314 18 -0.6137766842031951 2314 19 -0.7180762777313134 2314 20 -0.7357211596082678 2314 21 -0.7239218502903242 2314 22 -0.7342852307387062 2314 23 -0.9179179775424657 2314 24 -0.860174151608426 2314 25 -0.9612171022849836 2315 1 -0.39838952316958043 2315 2 -0.3310115198391266 2315 3 -0.43655340634200357 2315 4 -0.5177083789119123 2315 5 -0.4395140104775095 2315 6 -0.5392700969417703 2315 7 -0.5698601670136441 2315 8 -0.4909108988181927 2315 9 -0.5617124584903053 2315 10 -0.633167038357854 2315 11 -0.5283812547630902 2315 12 -0.5811716056333296 2315 13 -0.489845733754926 2315 14 -0.7980341445044346 2315 15 -0.769131403966071 2315 16 -0.854874846673134 2315 17 -0.8196363240769173 2315 18 -0.9461330447245189 2315 19 -0.858224902805766 2315 20 -0.7548195191380884 2315 21 -0.7284044534643712 2315 22 -0.924102877187849 2315 23 -0.815279763840536 2315 24 -0.6550067392356524 2315 25 -0.5954265934607577 2316 1 -0.8500308959611047 2316 2 -0.8010980930873401 2316 3 -0.995555239008155 2316 4 -0.8198264118588057 2316 5 -0.9533938559016875 2316 6 -1.0275066149017675 2316 7 -1.0122344393090543 2316 8 -1.026524983249267 2316 9 -1.3360732407189673 2316 10 -1.3752612094006977 2316 11 -1.3435944386577177 2316 12 -1.428573573405636 2316 13 -1.3406774970571647 2316 14 -1.7234153506222256 2316 15 -1.5778732343462067 2316 16 -1.546014991051777 2316 17 -1.6897403635196895 2316 18 -1.611780892850824 2316 19 -1.550778781521025 2316 20 -1.5448423407761354 2316 21 -1.4367335165699942 2316 22 -1.6713229910520297 2316 23 -1.6460444107216006 2316 24 -1.59729573540465 2316 25 -1.6128537459444914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34932.0 3 2.0 4 638.0 5 5836.0 6 7195.0 7 5860.0 8 4849.0 9 4384.0 10 4143.0 11 3991.0 12 3659.0 13 3642.0 14 3662.0 15 3594.0 16 3770.0 17 3957.0 18 4341.0 19 4934.0 20 5719.0 21 7588.0 22 10017.0 23 12617.0 24 15706.0 25 18530.0 26 21926.0 27 25026.0 28 29664.0 29 36706.0 30 47286.0 31 61424.0 32 79766.0 33 104594.0 34 136606.0 35 182299.0 36 264058.0 37 452418.0 38 990610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50284303531499 19.82844669376228 13.806352952602206 24.862357318320523 2 14.171958085135202 20.293183020849604 42.43294861104342 23.10191028297177 3 19.4491526887134 24.02199736065441 29.839877910120265 26.68897204051192 4 12.46190926990513 17.0885922940165 38.34756551260213 32.10193292347625 5 12.792754059910674 38.03895901990177 36.222099810176026 12.946187110011536 6 31.914362099949003 37.68465921627783 17.6775907740328 12.723387909740365 7 28.271811919573253 31.403070436144375 21.61780602766977 18.707311616612603 8 24.228563183700064 39.37195240582222 19.975371735977948 16.424112674499767 9 25.665237500810644 15.978785463568165 20.555736125304065 37.80024091031712 10 14.667823506906696 30.010871279522355 34.46920872204329 20.852096491527654 11 34.343150997966575 22.53206029741948 24.794192058248264 18.33059664636568 12 23.389598184768772 24.623582426210184 32.2249591223773 19.761860266643744 13 29.22428643077819 22.212407073200588 25.87099747539188 22.692309020629338 14 19.96566318066854 23.226740047483663 27.682544823402143 29.125051948445652 15 22.277987788709602 32.71541384731628 23.22954133024092 21.7770570337332 16 21.081609808941003 28.972209356361155 29.148997159959766 20.797183674738072 17 20.81049936126916 29.685577116052542 28.59990736580033 20.904016156877976 18 20.465404349816517 28.17522522505237 31.42644004161248 19.932930383518634 19 23.033374789759893 27.21377125952964 27.899011070439215 21.85384288027126 20 22.86345588497703 28.15527088212394 29.06200389953909 19.91926933335994 21 21.988381200092558 27.474789414528068 27.611591784797017 22.92523760058236 22 22.10918172228236 29.269030207421558 28.15112651859265 20.47066155170343 23 21.44190082000837 28.708236423659866 28.554204245746945 21.29565851058482 24 22.601785376459784 27.235030309495695 29.580855189414685 20.582329124629837 25 21.992376100113866 28.18559548813721 29.06319209840687 20.758836313342055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 245.0 2 400.0 3 555.0 4 555.0 5 555.0 6 1373.0 7 2191.0 8 2191.0 9 2191.0 10 3584.0 11 4977.0 12 4977.0 13 4977.0 14 8342.0 15 11707.0 16 11707.0 17 11707.0 18 21031.5 19 30356.0 20 30356.0 21 30356.0 22 50427.5 23 70499.0 24 70499.0 25 70499.0 26 102894.5 27 135290.0 28 135290.0 29 135290.0 30 179498.0 31 223706.0 32 223706.0 33 223706.0 34 249476.5 35 275247.0 36 275247.0 37 275247.0 38 291969.0 39 308691.0 40 308691.0 41 308691.0 42 323666.5 43 338642.0 44 338642.0 45 338642.0 46 340265.0 47 341888.0 48 341888.0 49 341888.0 50 323074.5 51 304261.0 52 304261.0 53 304261.0 54 264794.0 55 225327.0 56 225327.0 57 225327.0 58 189716.5 59 154106.0 60 154106.0 61 154106.0 62 124238.0 63 94370.0 64 94370.0 65 94370.0 66 71934.5 67 49499.0 68 49499.0 69 49499.0 70 35691.0 71 21883.0 72 21883.0 73 21883.0 74 15130.5 75 8378.0 76 8378.0 77 8378.0 78 5649.0 79 2920.0 80 2920.0 81 2920.0 82 1932.0 83 944.0 84 944.0 85 944.0 86 579.5 87 215.0 88 215.0 89 215.0 90 127.5 91 40.0 92 40.0 93 40.0 94 25.5 95 11.0 96 11.0 97 11.0 98 6.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4292869891160571 2 0.0 3 0.0 4 0.0 5 0.03468985770634805 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007521252334562188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2605949.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.6434613940098 #Duplication Level Percentage of deduplicated Percentage of total 1 85.18737154567371 61.031181645896424 2 10.529222992430523 15.08699961934233 3 2.344151280501879 5.038293352990651 4 0.7639012289383024 2.189141128171116 5 0.3506785777750261 1.2561913574265673 6 0.19904045348053478 0.8555968226867338 7 0.12321848358489197 0.6179459070219846 8 0.07988897229289126 0.4578818001818299 9 0.06445155890357337 0.41557794948827176 >10 0.3116414855020507 3.9782918915582774 >50 0.023824571517190272 1.1903590322451132 >100 0.018118002344626873 2.673035451125314 >500 0.0021384985975875163 1.0623159665003072 >1k 0.0020850361325860105 2.8262545712771825 >5k 2.673123246905142E-4 1.320933504087876 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 8712 0.3343119915240091 No Hit CTGTAGGACGTGGAATATGGCAAGA 7925 0.30411186097655785 No Hit GTATCAACGCAGAGTACTTTTTTTT 7093 0.2721849122910694 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5427 0.20825426744729078 No Hit CTTTAGGACGTGAAATATGGCGAGG 5330 0.20453201501641052 No Hit GGTATCAACGCAGAGTACTTTTTTT 4799 0.18415556098757113 No Hit TATCAACGCAGAGTACTTTTTTTTT 4573 0.17548309656098413 No Hit GTCCTACAGTGTGCATTTCTCATTT 4042 0.1551066425321447 No Hit CTGTAGGACCTGGAATATGGCGAGA 3119 0.11968768383418095 No Hit TTGTAGAACAGTGTATATCAATGAG 3035 0.11646428997651144 No Hit CTGAAGGACCTGGAATATGGCGAGA 3013 0.11562006777569324 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.837373640082749E-5 0.0 5 0.0 0.0 0.0 3.837373640082749E-5 0.0 6 0.0 0.0 0.0 3.837373640082749E-5 0.0 7 0.0 0.0 0.0 7.674747280165498E-5 0.0 8 0.0 0.0 0.0 7.674747280165498E-5 0.0 9 0.0 0.0 0.0 1.1512120920248247E-4 0.0 10 0.0 0.0 0.0 1.1512120920248247E-4 0.0 11 0.0 0.0 0.0 1.1512120920248247E-4 0.0 12 0.0 0.0 0.0 1.1512120920248247E-4 0.0 13 0.0 0.0 0.0 1.1512120920248247E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCGTT 30 7.739983E-4 18.994396 11 TAGGACC 1270 0.0 14.811204 4 GACCGTC 45 6.779643E-4 14.77342 7 GGACCGT 110 1.8735591E-10 13.814107 6 CCAACGA 225 0.0 12.241069 19 CCGAACT 55 0.0030691253 12.091056 4 GGTATCA 1885 0.0 11.790596 1 TGTAGGA 4140 0.0 11.771882 2 GTAGGAC 4105 0.0 11.455681 3 TCCAACG 335 0.0 11.339939 18 CTGTAGG 4175 0.0 11.21802 1 ACTGTTC 1035 0.0 11.103004 8 ATCCCGT 240 0.0 11.080065 10 AGGACCT 2655 0.0 10.985066 5 CCGTTTC 240 0.0 10.684348 13 GGACCTG 2605 0.0 10.68207 6 AGCACCG 125 2.2415225E-7 10.640128 5 CGTTTCC 250 0.0 10.636863 14 TGTAGAA 1300 0.0 10.596283 2 GACGTGG 2045 0.0 10.588564 7 >>END_MODULE