FastQCFastQC Report
Thu 26 May 2016
SRR1512229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541154
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54940.2162009858513101No Hit
CTGTAGGACGTGGAATATGGCAAGA46260.1820432764011941No Hit
GTATCAACGCAGAGTACTTTTTTTT44450.17492052823244872No Hit
GTCCTAAAGTGTGTATTTCTCATTT38130.15004993794158086No Hit
CTTTAGGACGTGAAATATGGCGAGG34580.13607990700288136No Hit
GTCCTACAGTGTGCATTTCTCATTT27850.10959587651909329No Hit
GGTATCAACGCAGAGTACTTTTTTT27180.10695927913066268No Hit
TATCAACGCAGAGTACTTTTTTTTT26080.1026305371496572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG856.5992936E-915.6219551
CGGTTAA400.003261064915.3215311
GGCGTAG450.0071275313.6191391
TCCAACG3000.013.50679618
TAGGACC9500.013.389494
GCGTATA550.001780984713.0000871
CGTTAGG654.3621543E-412.5715121
ACCGACC550.003265146611.9914638
TGTAGGA26250.011.907022
GACCGTC658.5992645E-411.5965957
CCAACGA2200.011.56501619
CTGTAGG27300.011.2619811
GTAGGAC28550.011.1548693
GGCGAGG11700.010.87309219
ACGTGTC1053.82902E-610.7678448
GTCCTAG3900.010.7381671
GTCCTAC28100.010.6505551
AGGACGT27850.010.4541125
GGACGTG27400.010.4538656
TAGGACG28500.010.3809874
Produced by FastQC (version 0.11.5)