FastQCFastQC Report
Thu 26 May 2016
SRR1512229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541154
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60870.23953684034891234No Hit
GTATCAACGCAGAGTACTTTTTTTT60160.23674283416117245No Hit
CTGTAGGACGTGGAATATGGCAAGA52780.2077009106886084No Hit
GTCCTAAAGTGTGTATTTCTCATTT42670.16791583666318532No Hit
CTTTAGGACGTGAAATATGGCGAGG41330.16264264188632407No Hit
GGTATCAACGCAGAGTACTTTTTTT38540.1516633781345011No Hit
TATCAACGCAGAGTACTTTTTTTTT36860.14505220856351092No Hit
GTCCTACAGTGTGCATTTCTCATTT32440.12765853623983434No Hit
CTGTAGGACCTGGAATATGGCGAGA26540.10444073834171405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9050.013.4359074
TAAAGCG701.09184824E-412.213785
CCCGTTT1302.6320777E-911.68860212
CCGTTTC1501.7826096E-1011.39638713
AATCCCG3150.011.1571719
TGTAGGA28300.011.1444142
GTATTAG2150.011.0956261
GGCGAGG11250.010.97624319
AGGACCT18150.010.8866015
TACACCG700.001492482310.8566935
GTAGGAC27900.010.8275083
CTGTAGG28550.010.7955971
CCAACGA1951.8189894E-1210.7164519
GTCGAGG1252.2461245E-710.63851219
TCCAACG2500.010.63662818
AGGACGT27300.010.543525
GGACGTG27200.010.4397056
TAGGACT4650.010.4189234
GTCCTAC30100.010.2713791
GGACCTG18150.010.2557026
Produced by FastQC (version 0.11.5)