FastQCFastQC Report
Thu 26 May 2016
SRR1512228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158420
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65760.30466730293455396No Hit
GTATCAACGCAGAGTACTTTTTTTT58870.2727458048016605No Hit
CTGTAGGACGTGGAATATGGCAAGA58520.2711242482927327No Hit
GTCCTAAAGTGTGTATTTCTCATTT54180.2510169475820276No Hit
CTTTAGGACGTGAAATATGGCGAGG46990.2177055438700531No Hit
GGTATCAACGCAGAGTACTTTTTTT36280.1680859146968616No Hit
TATCAACGCAGAGTACTTTTTTTTT35390.16396252814558795No Hit
CTGAAGGACCTGGAATATGGCGAGA25750.1193002288711187No Hit
GTCCTACAGTGTGCATTTCTCATTT22790.10558649382418621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA808.971838E-714.0270861
TCCAACG2250.013.40297418
CGTACAT600.00345338311.901772
TAGGACC9000.011.5179144
CCAACGT1157.912422E-811.47265419
GGCGAGG14000.010.90487519
ACTGTTC7300.010.7144248
CACCTTT7100.010.61830714
GGTATCA15950.010.4253751
TGTAGGA29900.010.4062312
GATATAC7000.010.3472531
AGGACGT32550.010.3067725
CCACCTT7150.010.28045313
GGACGTG32100.010.2751146
TGTAGAA8950.010.2585092
GTAGAAC8400.010.2090613
CTGTAGG30350.010.1174851
GTCCTAC30450.09.9502491
GTCCTAA15800.09.9432511
GACGTGG16150.09.9197357
Produced by FastQC (version 0.11.5)