FastQCFastQC Report
Thu 26 May 2016
SRR1512228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158420
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79260.3672130539931987No Hit
GTCCTACAGTGGACATTTCTAAATT69610.32250442453276007No Hit
CTGTAGGACGTGGAATATGGCAAGA64320.2979957561549652No Hit
GTCCTAAAGTGTGTATTTCTCATTT55020.25490868320345433No Hit
CTTTAGGACGTGAAATATGGCGAGG53220.2465692497289684No Hit
TATCAACGCAGAGTACTTTTTTTTT51650.23929541053177789No Hit
GGTATCAACGCAGAGTACTTTTTTT49370.22873212813076232No Hit
CTGAAGGACCTGGAATATGGCGAGA29730.13773964288692656No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26610.1232846248644842No Hit
GTCCTACAGTGTGCATTTCTCATTT23190.10743970126296087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.012.327174
GGCGAGG14050.012.30355819
TCCAACG2150.011.92698918
CCAACGA1354.7457434E-911.2569819
AGCGGTC600.005888818811.080328
ACCTTTT8100.011.02169415
CCACCTT8300.010.87053713
TTAGGAC20250.010.6492013
GCCGGTT1451.45028025E-810.47990911
TGTAGGA33050.010.4340132
TAGGACA8200.010.4266514
AGGACGT35200.010.4174365
TGGCGAG31600.010.36901518
GACGTGA18350.010.2996517
GGACGTG34950.010.244716
GTCCTAA18100.010.1685381
CACCTTT8500.010.16782414
AGGACCT19600.010.0329925
CTGTAGG33500.09.9632961
AATGTCC7650.09.931948
Produced by FastQC (version 0.11.5)