FastQCFastQC Report
Thu 26 May 2016
SRR1512227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858814
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT82580.28886104517467737No Hit
CTGTAGGACGTGGAATATGGCAAGA69900.24450698786279904No Hit
GTCCTAAAGTGTGTATTTCTCATTT65370.22866125603134727No Hit
CTTTAGGACGTGAAATATGGCGAGG58270.20382578229993278No Hit
GTATCAACGCAGAGTACTTTTTTTT41000.14341611591380202No Hit
GTCCTACAGTGTGCATTTCTCATTT31850.11140983638669744No Hit
CTGAAGGACCTGGAATATGGCGAGA29640.10367935794353882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1251.8189894E-1214.7589451
TCCAACG4800.012.9520618
CCAACGA3250.012.46296119
TAGTACG550.003273985611.9872154
CGTTAGG1301.0657459E-811.8260771
GGCGAGG17550.011.808145519
TAGGACC12300.011.5625824
GTCCTAG4450.010.8250681
CGGTTTC2700.010.8148113
GTCCTAC37500.010.7958951
TGTAGGA37200.010.7727512
AACCGTG700.001603172710.764037
TATACCG700.001603172710.764035
GTCTAAG2500.010.6592381
CTGTAGG36500.010.642391
CCAACGT2150.010.51499219
TGTCGAG2150.010.51499218
AGGACGT41950.010.4176315
TCCTAGA5250.010.346882
GTAGGAC39600.010.3461093
Produced by FastQC (version 0.11.5)