FastQCFastQC Report
Thu 26 May 2016
SRR1512227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858814
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT87980.30774999702673905No Hit
CTGTAGGACGTGGAATATGGCAAGA83440.2918692856548205No Hit
CTTTAGGACGTGAAATATGGCGAGG70020.24492674234840042No Hit
GTCCTAAAGTGTGTATTTCTCATTT65940.2306550898379538No Hit
GTATCAACGCAGAGTACTTTTTTTT52370.183187853424532No Hit
CTGAAGGACCTGGAATATGGCGAGA36860.1289345861605547No Hit
GTCCTACAGTGTGCATTTCTCATTT35850.12540165257340982No Hit
TATCAACGCAGAGTACTTTTTTTTT31640.11067526603689502No Hit
GGTATCAACGCAGAGTACTTTTTTT31440.1099756752275594No Hit
CTGTAGGACCTGGAATATGGCGAGA28610.10007646527546038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4550.013.35860318
CCAACGA3050.013.07984719
GGCGAGG20150.012.02038519
TAGGACC11400.011.83389954
ACTGTTC11950.010.9673998
GGACGTG43850.010.9157456
AGGACGT45050.010.8817645
GTCTAGG2200.010.8376871
GACGTGA23900.010.8084517
ACCTTTT8750.010.74532715
GACGTGG21300.010.6118187
CCGTTTC3500.010.58251913
TTAGGAC26950.010.5403943
CCTACAC4150.010.5306253
CCCGTTT3550.010.43346912
AAGTCGT2650.010.3930877
TGTAGGA41600.010.2769352
ACGTGAA26450.010.2332058
GAACAGT8500.010.1675186
ATCCCGT3650.010.1476210
Produced by FastQC (version 0.11.5)