FastQCFastQC Report
Thu 26 May 2016
SRR1512226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712360
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT45170.16653394092229645No Hit
CTGTAGGACGTGGAATATGGCAAGA40200.14821041454674158No Hit
GTATCAACGCAGAGTACTTTTTTTT38600.14231149257473197No Hit
GTCCTAAAGTGTGTATTTCTCATTT29450.10857703254730199No Hit
CTTTAGGACGTGAAATATGGCGAGG28140.10374729018271911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3600.013.61011118
CCAACGA2800.012.45100919
TAGGACC8200.012.2943834
ACCGTCG550.00326479211.9916848
GTATACG750.001445200810.8986551
GTCCTAC22600.010.71481
AATCCCG5200.010.69078319
GTAGGAC22200.010.3980043
TCCTACA24550.010.2799172
TGTAGAA13350.010.1026852
TGTAGGA22100.010.0325572
AGGACCT14850.010.0246095
GATATAC11200.09.9438061
TTATACT2950.09.9009424
ACTGTTC12250.09.8450688
ACCGTGC2300.09.8316928
GTCCTAG3850.09.8194061
GTCTTAT2500.09.8087891
GCACCGT1357.2161856E-79.7710036
GGACCTG14700.09.7425156
Produced by FastQC (version 0.11.5)