FastQCFastQC Report
Thu 26 May 2016
SRR1512226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712360
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51940.19149375451636214No Hit
GTCCTACAGTGGACATTTCTAAATT51710.19064578448288574No Hit
CTGTAGGACGTGGAATATGGCAAGA44770.16505921042929403No Hit
GTCCTAAAGTGTGTATTTCTCATTT35660.1314722234511643No Hit
GGTATCAACGCAGAGTACTTTTTTT34120.12579451105310505No Hit
TATCAACGCAGAGTACTTTTTTTTT33400.12313999616570073No Hit
CTTTAGGACGTGAAATATGGCGAGG32220.11878954121134362No Hit
TTGTAGAACAGTGTATATCAATGAG27440.1011665118199649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7750.012.8713934
TCCAACG4050.012.66288118
GGTATCA16400.011.3404381
ACTGTTC11950.011.0469078
CCAACGT2150.011.04443219
GATATAC10550.011.0292631
TGTAGGA22850.011.0178692
GTAGGAC22050.010.9867573
GGCGAGG8150.010.95499219
TAGTACC1658.0035534E-1110.9397554
CTGTAGG23800.010.7398181
CGTGCCG803.783059E-410.68430610
GTCCTAC22450.010.5359671
GTCTTAC2100.010.4459421
GCCGGTT2550.010.42825611
CCGGTTT2550.010.42825612
GTATTAG2500.010.3006081
ACCGTCC1201.5306578E-610.2885918
AAGTCGT1201.5306578E-610.2885917
GTACTAG1409.152609E-810.2188561
Produced by FastQC (version 0.11.5)