##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512226_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2712360 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24056467430577 31.0 31.0 34.0 28.0 34.0 2 31.428208276187526 33.0 31.0 34.0 28.0 34.0 3 31.525046822693152 33.0 31.0 34.0 28.0 34.0 4 35.10141352917754 37.0 35.0 37.0 32.0 37.0 5 34.76240911973337 37.0 35.0 37.0 32.0 37.0 6 34.71361766137238 37.0 35.0 37.0 32.0 37.0 7 34.56000899585601 37.0 35.0 37.0 31.0 37.0 8 34.48160458051291 37.0 35.0 37.0 30.0 37.0 9 36.03483350292734 39.0 37.0 39.0 31.0 39.0 10 35.868359657272634 39.0 35.0 39.0 30.0 39.0 11 35.864233730035835 39.0 35.0 39.0 30.0 39.0 12 35.69818571281098 39.0 35.0 39.0 30.0 39.0 13 35.686063428158505 39.0 35.0 39.0 30.0 39.0 14 36.77790263829285 40.0 36.0 41.0 30.0 41.0 15 36.7260750785294 40.0 36.0 41.0 30.0 41.0 16 36.63634989455677 40.0 36.0 41.0 30.0 41.0 17 36.57962475482606 40.0 36.0 41.0 30.0 41.0 18 36.51830288014865 40.0 36.0 41.0 30.0 41.0 19 36.54148306272029 40.0 36.0 41.0 30.0 41.0 20 36.426094250025805 40.0 36.0 41.0 30.0 41.0 21 36.333257384712944 40.0 36.0 41.0 30.0 41.0 22 36.42341024052855 40.0 36.0 41.0 30.0 41.0 23 36.332760769219426 40.0 36.0 41.0 30.0 41.0 24 36.22284689348022 40.0 36.0 41.0 30.0 41.0 25 35.80547235617691 39.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21001561596912666 1101 2 0.18917095905460712 1101 3 0.19932216610876097 1101 4 0.11305013577678125 1101 5 0.1508215030293769 1101 6 0.09842000078324986 1101 7 0.2093478826604951 1101 8 -0.01533533716410318 1101 9 0.18682764694465703 1101 10 0.2763478738967535 1101 11 0.25082150750405674 1101 12 0.16039727259484238 1101 13 0.18115814469476987 1101 14 0.17436161343117362 1101 15 0.0984331382068504 1101 16 0.14795804684944613 1101 17 0.1190675837421864 1101 18 0.30748541577293764 1101 19 0.18778142819649446 1101 20 0.2287910353643383 1101 21 0.019747987312641158 1101 22 0.19108348987464296 1101 23 -0.0277600575265069 1101 24 -0.05508168459416396 1101 25 0.04221938730105279 1102 1 0.17422997304062804 1102 2 0.2929099490844358 1102 3 0.29473140133221776 1102 4 0.2331784059691131 1102 5 0.24541477010823343 1102 6 0.3523632068404865 1102 7 0.3395995976335442 1102 8 0.3494003435549189 1102 9 0.39593900323730935 1102 10 0.332173854450474 1102 11 0.47559785223555906 1102 12 0.5870265369883612 1102 13 0.5641907111033788 1102 14 0.5577068076347942 1102 15 0.648079200024597 1102 16 0.7109283884873037 1102 17 0.6059671351836968 1102 18 0.7252633457225528 1102 19 0.5497626564493459 1102 20 0.6257179393380028 1102 21 0.5711264614970091 1102 22 0.5675827113450396 1102 23 0.54635208913529 1102 24 0.6726563045147458 1102 25 0.4955389697045618 1103 1 0.14924233328019554 1103 2 0.1056552588453421 1103 3 0.21157297802232122 1103 4 0.2981145256521174 1103 5 0.23634724266973706 1103 6 0.3092822802216304 1103 7 0.323004854463548 1103 8 0.4322472083855686 1103 9 0.4896532877140203 1103 10 0.38038930812231797 1103 11 0.34504108145411294 1103 12 0.49643990362060464 1103 13 0.44232540089694083 1103 14 0.5098807247138311 1103 15 0.47391120348484606 1103 16 0.6363619559896847 1103 17 0.43097383353630647 1103 18 0.6262035283508922 1103 19 0.5844686312561151 1103 20 0.3918724166530225 1103 21 0.7225367663787949 1103 22 0.7686279637445352 1103 23 0.8394904081055827 1103 24 0.6418426449494632 1103 25 0.6218135904045425 1104 1 0.29320601048027584 1104 2 0.27382402153489593 1104 3 0.27823870265623185 1104 4 0.20234549225209975 1104 5 0.31297704610716437 1104 6 0.42046693599542806 1104 7 0.4147724301918245 1104 8 0.3459487277852489 1104 9 0.5260995435056657 1104 10 0.5489523858920791 1104 11 0.5352101267534977 1104 12 0.49928950313272935 1104 13 0.5897678563301199 1104 14 0.7871458052948128 1104 15 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-0.6733585289900148 2314 13 -0.7569396220223368 2314 14 -0.6892286568825625 2314 15 -0.5683713558985701 2314 16 -0.6069824397538213 2314 17 -0.7230241920066192 2314 18 -0.738323201389889 2314 19 -0.6280674084610496 2314 20 -0.693459032482707 2314 21 -0.7485807670725251 2314 22 -0.5360802425285982 2314 23 -0.7508427530846973 2314 24 -0.6122837565568915 2314 25 -0.6099888448758577 2315 1 -0.15472720699737863 2315 2 -0.11528223467165688 2315 3 -0.14795171442420596 2315 4 -0.18307531211731742 2315 5 -0.19629802837147992 2315 6 -0.1994374830711081 2315 7 -0.27315085679924067 2315 8 -0.16366150061594453 2315 9 -0.2861646074377333 2315 10 -0.21482740556770352 2315 11 -0.2895192674868241 2315 12 -0.43544361504006446 2315 13 -0.3283999688933221 2315 14 -0.5629957041391478 2315 15 -0.4144151195655894 2315 16 -0.5608084742739905 2315 17 -0.5839449733844262 2315 18 -0.6139154735784516 2315 19 -0.5949483656113017 2315 20 -0.49922474016294416 2315 21 -0.43150356824768465 2315 22 -0.5009426542667939 2315 23 -0.4008495344291134 2315 24 -0.27125119775742235 2315 25 -0.44631170781341467 2316 1 -0.6800868333949808 2316 2 -0.6522095594603137 2316 3 -0.6094614635379223 2316 4 -0.5491221487685252 2316 5 -0.6845684128819798 2316 6 -0.7151707118684882 2316 7 -0.7002182824878105 2316 8 -0.6019523378125626 2316 9 -0.7978226385258154 2316 10 -0.8828581527724992 2316 11 -0.9685211764041952 2316 12 -1.0296163038865274 2316 13 -1.045529093518354 2316 14 -1.2249775694241194 2316 15 -1.121036676696761 2316 16 -1.015357492545057 2316 17 -1.1363396420509062 2316 18 -1.096569891358648 2316 19 -1.1670440841823435 2316 20 -1.0261694497348017 2316 21 -1.1948029273968501 2316 22 -1.1955465539122798 2316 23 -1.0866741185578306 2316 24 -1.0120440802226511 2316 25 -1.071132746809866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30623.0 3 1.0 4 545.0 5 5125.0 6 6608.0 7 5288.0 8 4388.0 9 3981.0 10 3727.0 11 3521.0 12 3409.0 13 3358.0 14 3184.0 15 3311.0 16 3578.0 17 3666.0 18 3893.0 19 4374.0 20 5237.0 21 6864.0 22 9240.0 23 11384.0 24 14470.0 25 17233.0 26 20580.0 27 23441.0 28 27714.0 29 34539.0 30 44991.0 31 58275.0 32 76279.0 33 100745.0 34 132219.0 35 177149.0 36 256478.0 37 441940.0 38 1161002.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67851885864947 18.743596057745183 13.44813987117129 25.129745212434063 2 14.957454025276881 19.710252326387355 40.90754914539368 24.424744502942087 3 19.47982568685573 23.21834122314147 29.409923461487413 27.89190962851539 4 12.809840876579806 16.419428099514814 37.147686885221724 33.62304413868365 5 13.660112905504059 37.21795291190649 35.462964567941164 13.658969614648283 6 33.19360261912136 36.37028270583551 17.3150687961775 13.121045878865637 7 29.19247445029421 30.970262059608604 20.923881785603683 18.913381704493503 8 25.43139553746553 37.56024274063915 19.527127667418778 17.481234054476545 9 26.040127416714597 15.80162662773378 19.688205105516968 38.47004085003466 10 15.041366190328716 29.289216770635168 33.65950685012314 22.009910188912976 11 35.3661755814125 22.200887787756788 23.56818416434397 18.86475246648675 12 23.50410712442301 24.15239864914687 31.26701470306302 21.076479523367105 13 29.371617336931678 21.284785205503695 25.51980563052102 23.823791827043607 14 21.08665516376882 21.752459113097082 27.041469421463226 30.11941630167087 15 23.356449733811147 30.47855004497928 23.01847837307732 23.146521848132252 16 22.617720361603915 28.081486233390844 27.144589951186422 22.156203453818815 17 21.99892344674011 28.243153563686235 27.420880709050422 22.337042280523235 18 21.76753823238803 27.591617631877774 29.146905278060437 21.49393885767376 19 23.698218525564453 26.749362179061777 26.95025734047103 22.60216195490274 20 23.544625344718252 27.243212552905955 27.7598106446047 21.452351457771094 21 23.365482458080784 26.63112566178531 26.753454556179857 23.249937323954047 22 23.126502381689747 27.544315651314722 27.23502779866979 22.094154168325737 23 22.918897196537333 27.12670884395877 27.22433600259553 22.730057956908375 24 23.251043371823798 26.512815407984192 27.967305224970136 22.268835995221874 25 23.115623839703908 26.946006664132373 27.672040915427758 22.266328580735962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 148.0 1 148.0 2 282.0 3 416.0 4 416.0 5 416.0 6 1113.5 7 1811.0 8 1811.0 9 1811.0 10 2767.5 11 3724.0 12 3724.0 13 3724.0 14 6341.5 15 8959.0 16 8959.0 17 8959.0 18 16666.5 19 24374.0 20 24374.0 21 24374.0 22 40952.5 23 57531.0 24 57531.0 25 57531.0 26 84934.5 27 112338.0 28 112338.0 29 112338.0 30 150596.0 31 188854.0 32 188854.0 33 188854.0 34 215057.5 35 241261.0 36 241261.0 37 241261.0 38 265633.0 39 290005.0 40 290005.0 41 290005.0 42 313354.0 43 336703.0 44 336703.0 45 336703.0 46 347990.0 47 359277.0 48 359277.0 49 359277.0 50 352492.5 51 345708.0 52 345708.0 53 345708.0 54 313131.5 55 280555.0 56 280555.0 57 280555.0 58 242095.5 59 203636.0 60 203636.0 61 203636.0 62 167529.5 63 131423.0 64 131423.0 65 131423.0 66 102088.0 67 72753.0 68 72753.0 69 72753.0 70 52945.0 71 33137.0 72 33137.0 73 33137.0 74 23113.0 75 13089.0 76 13089.0 77 13089.0 78 8959.0 79 4829.0 80 4829.0 81 4829.0 82 3103.0 83 1377.0 84 1377.0 85 1377.0 86 869.0 87 361.0 88 361.0 89 361.0 90 215.0 91 69.0 92 69.0 93 69.0 94 43.0 95 17.0 96 17.0 97 17.0 98 11.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4378106151100887 2 0.0 3 0.0 4 0.0 5 0.032775885206978425 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00844283207243876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2712360.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.47918852134055 #Duplication Level Percentage of deduplicated Percentage of total 1 85.06266427783508 61.65272880320708 2 10.253716737315163 14.86362136896581 3 2.379559567348937 5.174056394389295 4 0.8779784672735028 2.5454066738877534 5 0.425233039547803 1.541027281944394 6 0.25127417001052554 1.0927288763241754 7 0.15755501581454767 0.7993621785593775 8 0.102067308652711 0.5918204564564555 9 0.07735459964848909 0.5045938749824097 >10 0.3710492869088212 4.711531111614973 >50 0.023454284764490636 1.1693823914676615 >100 0.014536858584769885 1.9812669775576375 >500 0.0018798041846834481 0.9180080484619806 >1k 0.0015749710734860051 2.0727907358062456 >5k 1.016110369990971E-4 0.3816748263746978 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5194 0.19149375451636214 No Hit GTCCTACAGTGGACATTTCTAAATT 5171 0.19064578448288574 No Hit CTGTAGGACGTGGAATATGGCAAGA 4477 0.16505921042929403 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3566 0.1314722234511643 No Hit GGTATCAACGCAGAGTACTTTTTTT 3412 0.12579451105310505 No Hit TATCAACGCAGAGTACTTTTTTTTT 3340 0.12313999616570073 No Hit CTTTAGGACGTGAAATATGGCGAGG 3222 0.11878954121134362 No Hit TTGTAGAACAGTGTATATCAATGAG 2744 0.1011665118199649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.37365246501202E-5 0.0 0.0 0.0 0.0 7 7.37365246501202E-5 3.68682623250601E-5 0.0 0.0 0.0 8 7.37365246501202E-5 3.68682623250601E-5 0.0 0.0 0.0 9 7.37365246501202E-5 3.68682623250601E-5 0.0 3.68682623250601E-5 0.0 10 7.37365246501202E-5 3.68682623250601E-5 0.0 3.68682623250601E-5 0.0 11 7.37365246501202E-5 3.68682623250601E-5 0.0 3.68682623250601E-5 0.0 12 7.37365246501202E-5 3.68682623250601E-5 0.0 3.68682623250601E-5 0.0 13 1.1060478697518028E-4 3.68682623250601E-5 0.0 3.68682623250601E-5 3.68682623250601E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 775 0.0 12.871393 4 TCCAACG 405 0.0 12.662881 18 GGTATCA 1640 0.0 11.340438 1 ACTGTTC 1195 0.0 11.046907 8 CCAACGT 215 0.0 11.044432 19 GATATAC 1055 0.0 11.029263 1 TGTAGGA 2285 0.0 11.017869 2 GTAGGAC 2205 0.0 10.986757 3 GGCGAGG 815 0.0 10.954992 19 TAGTACC 165 8.0035534E-11 10.939755 4 CTGTAGG 2380 0.0 10.739818 1 CGTGCCG 80 3.783059E-4 10.684306 10 GTCCTAC 2245 0.0 10.535967 1 GTCTTAC 210 0.0 10.445942 1 GCCGGTT 255 0.0 10.428256 11 CCGGTTT 255 0.0 10.428256 12 GTATTAG 250 0.0 10.300608 1 ACCGTCC 120 1.5306578E-6 10.288591 8 AAGTCGT 120 1.5306578E-6 10.288591 7 GTACTAG 140 9.152609E-8 10.218856 1 >>END_MODULE