FastQCFastQC Report
Thu 26 May 2016
SRR1512225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880303
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79900.2774013706196883No Hit
CTGTAGGACGTGGAATATGGCAAGA73270.25438295901507585No Hit
GTCCTAAAGTGTGTATTTCTCATTT66760.23178117024493602No Hit
CTTTAGGACGTGAAATATGGCGAGG58750.20397159604388843No Hit
GTATCAACGCAGAGTACTTTTTTTT45560.15817780282143926No Hit
GTCCTACAGTGTGCATTTCTCATTT31820.1104744882743239No Hit
CTGAAGGACCTGGAATATGGCGAGA30480.10582219995604629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1600.012.7746381
CGTTAGG1051.0528129E-712.6529751
TAGGACC11400.012.6453244
GGACCGT701.17880554E-412.1140076
GTCTAAT1301.112312E-811.7919741
GGCGAGG19350.011.73551219
TACGACT658.601623E-411.5963144
GGTCTAC2650.011.1838341
TACATCG855.7926474E-511.0847125
TGTAGGA39700.011.0692072
CGGAATA600.006252769410.99157511
TCCAACG3750.010.80446218
TCTAGAC2100.010.7680063
CTGTAGG39900.010.73197651
AGGACGT46150.010.6163445
CCACCTT10350.010.55974613
TGTAGAA12250.010.5117032
GGTATCA15750.010.5117021
GTAGGAC42200.010.4937033
GTCCTAC38250.010.4735341
Produced by FastQC (version 0.11.5)