FastQCFastQC Report
Thu 26 May 2016
SRR1512225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880303
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT88840.3084397717878987No Hit
CTGTAGGACGTGGAATATGGCAAGA78530.2726449265927925No Hit
GTCCTAAAGTGTGTATTTCTCATTT70360.24427985527911475No Hit
CTTTAGGACGTGAAATATGGCGAGG66970.2325102602052631No Hit
GTATCAACGCAGAGTACTTTTTTTT60580.21032509426959595No Hit
GGTATCAACGCAGAGTACTTTTTTT38950.1352288283559056No Hit
TATCAACGCAGAGTACTTTTTTTTT38890.1350205169386693No Hit
GTCCTACAGTGTGCATTTCTCATTT35520.12332035900389647No Hit
CTGAAGGACCTGGAATATGGCGAGA34920.12123724483153334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3800.015.24537518
CCAACGA3100.012.86773419
TAGGACC11400.012.6672224
GGCGAGG19450.010.88929419
GGTATCA16700.010.7377811
AATGTCC9350.010.6652148
AATCCCG5550.010.60994619
TGTAGGA42900.010.5855462
TGTAGAA14450.010.4537462
CACCTTT10750.010.42474514
ACTGTTC12200.010.3534168
GATATAC11650.010.3161531
GTCCTAG3550.010.2100621
TACGTCC750.002647780310.1337795
GTAGGAC42900.010.120493
GCCGGTT2450.010.07857511
TCTAGAC2550.010.0592653
CTGTAGG43700.010.0403661
CCACCTT11150.09.96558713
TTAGGAC26050.09.9562913
Produced by FastQC (version 0.11.5)