FastQCFastQC Report
Thu 26 May 2016
SRR1512224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665831
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66570.24971575467462115No Hit
CTGTAGGACGTGGAATATGGCAAGA60870.22833405418422997No Hit
GTCCTAAAGTGTGTATTTCTCATTT53990.20252596657477534No Hit
CTTTAGGACGTGAAATATGGCGAGG48440.18170694241307872No Hit
GTATCAACGCAGAGTACTTTTTTTT44670.16756501068522348No Hit
GGTATCAACGCAGAGTACTTTTTTT27180.10195695075944425No Hit
TATCAACGCAGAGTACTTTTTTTTT27090.10161934496222755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG655.890635E-513.0466195
TAGGACC9950.012.0267824
CCAACGA1950.011.598319
GGCGAGG14150.011.58805519
TGTAGGA33350.011.1442952
CGGTAGG1206.0262937E-711.0613761
GTCTTAA2350.010.8622341
GTCCTAC32300.010.6530611
AGGACGT34850.010.6527625
TTAGGAC20000.010.64749053
CTGTAGG33600.010.6359391
GTAGGAC34800.010.5868393
GGACGTG35550.010.4430046
GACGTGA17600.010.3862297
CTGCACG1002.6285566E-510.3642319
TCCAACG3550.010.35270818
TCGTACA800.002486928610.2105012
TAGGACG38200.010.1625494
TGGCGAG32200.010.15526618
GGTATCA16700.010.149361
Produced by FastQC (version 0.11.5)