##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2665831 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.770420930659146 31.0 28.0 33.0 22.0 34.0 2 25.867020827651867 31.0 25.0 34.0 2.0 34.0 3 27.528987396425354 31.0 26.0 34.0 16.0 34.0 4 32.01635362481718 35.0 32.0 37.0 19.0 37.0 5 32.41945944810455 35.0 33.0 37.0 25.0 37.0 6 32.61981048311014 35.0 33.0 37.0 25.0 37.0 7 32.60492094210023 35.0 33.0 37.0 25.0 37.0 8 32.605778460825164 36.0 33.0 37.0 25.0 37.0 9 34.045799602450415 38.0 34.0 39.0 25.0 39.0 10 33.890793152304106 38.0 34.0 39.0 25.0 39.0 11 33.90269975853683 38.0 34.0 39.0 25.0 39.0 12 33.7730647591689 38.0 34.0 39.0 23.0 39.0 13 33.76627438123422 38.0 34.0 39.0 23.0 39.0 14 34.75032663360881 39.0 34.0 41.0 23.0 41.0 15 34.678953767136775 39.0 34.0 41.0 22.0 41.0 16 34.61776909338964 39.0 34.0 41.0 22.0 41.0 17 34.58916938095476 39.0 34.0 41.0 22.0 41.0 18 34.52744491304963 39.0 34.0 41.0 20.0 41.0 19 34.536176149200756 39.0 34.0 41.0 19.0 41.0 20 34.44099944820208 39.0 34.0 41.0 18.0 41.0 21 34.35445195138026 39.0 34.0 41.0 18.0 41.0 22 34.239269105956076 39.0 34.0 41.0 18.0 41.0 23 34.104650294786126 39.0 34.0 41.0 16.0 41.0 24 33.96705492583738 39.0 34.0 41.0 10.0 41.0 25 33.82568812501618 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6443267487617064 1101 2 3.626866293770913 1101 3 2.2067544834741994 1101 4 1.1688720564817388 1101 5 0.7360861726108183 1101 6 0.7921038811551284 1101 7 0.44183434337490013 1101 8 0.5293675270237799 1101 9 0.48930405831053747 1101 10 0.628221406347528 1101 11 0.7906783880655439 1101 12 0.6531611648496494 1101 13 0.5879537412228473 1101 14 0.7553715659784856 1101 15 0.8222190202536126 1101 16 0.8919117058961845 1101 17 0.7811690866344065 1101 18 0.7706761132049209 1101 19 0.6960728155485185 1101 20 0.8115331457014676 1101 21 0.7926949238764607 1101 22 0.8805961401913436 1101 23 0.6165518424317824 1101 24 0.6053009497096866 1101 25 0.6766791740882212 1102 1 1.5615937067044001 1102 2 4.298813890220977 1102 3 2.8363252727616697 1102 4 1.944814930313747 1102 5 1.4405556719335877 1102 6 1.4204538331645011 1102 7 1.4162349831628944 1102 8 1.2400758086585526 1102 9 1.2852545178641392 1102 10 1.3137862224509504 1102 11 1.2940721980905892 1102 12 1.5024089957971754 1102 13 1.4180391312275944 1102 14 1.6118905168882165 1102 15 1.6245114958842422 1102 16 1.4220773634201294 1102 17 1.4695370588161794 1102 18 1.6500201802363037 1102 19 1.6796619138015814 1102 20 1.6277223235359983 1102 21 1.8020522806216448 1102 22 1.7677155718567974 1102 23 1.7142338523801257 1102 24 1.6235894972302134 1102 25 1.5799254513022447 1103 1 0.9957131364050937 1103 2 3.6745825669085868 1103 3 2.2689520350343138 1103 4 1.2786706972468949 1103 5 0.8361241430344819 1103 6 0.5548472112402223 1103 7 0.42396424088697415 1103 8 0.733026090916951 1103 9 0.7252450538509052 1103 10 0.6167204114966509 1103 11 0.6285311469140495 1103 12 0.6308064605203612 1103 13 0.5740620756583468 1103 14 0.6804116346828835 1103 15 0.8243334106253357 1103 16 0.6483508501971613 1103 17 0.8961909562774508 1103 18 0.8450229115668293 1103 19 0.9390273043630302 1103 20 0.8532294071209847 1103 21 0.9289117647984284 1103 22 0.9637940585679772 1103 23 1.0671280279040118 1103 24 1.1467316436985229 1103 25 1.088660848405091 1104 1 1.1643599671612392 1104 2 4.049845499853706 1104 3 2.5695835012018016 1104 4 1.6934535767924146 1104 5 1.3218051847416348 1104 6 0.9838846791277049 1104 7 1.000529399293491 1104 8 0.92399556644218 1104 9 0.9686493456487426 1104 10 0.9874570081322673 1104 11 0.8716742980580463 1104 12 1.0202637303131326 1104 13 1.105212403289194 1104 14 1.2431932341510858 1104 15 0.954726491374231 1104 16 1.1124679583572927 1104 17 1.0886067727370587 1104 18 1.1033886408829474 1104 19 1.1680763344360017 1104 20 1.2013953363805498 1104 21 1.2292079580937667 1104 22 1.2996221596818813 1104 23 1.2533416012996383 1104 24 1.3585394417367311 1104 25 1.2849014907378802 1105 1 0.7787433400727437 1105 2 3.5296964076286876 1105 3 2.057826833620581 1105 4 1.2529970402448924 1105 5 0.8428852903254693 1105 6 0.5638492361675702 1105 7 0.3038987274737792 1105 8 0.3490089299873773 1105 9 0.48783431130561183 1105 10 0.6481497956022295 1105 11 0.48040033828586814 1105 12 0.3236493194444279 1105 13 0.3523803369303238 1105 14 0.5025580636653615 1105 15 0.46307929842829054 1105 16 0.3367546643498329 1105 17 0.4122502546383231 1105 18 0.46523648805129625 1105 19 0.5255969947133963 1105 20 0.539676630728593 1105 21 0.4584876630077659 1105 22 0.6054202826359614 1105 23 0.30631297966063187 1105 24 0.44978348135905577 1105 25 0.44235834895658144 1106 1 0.15794746492106881 1106 2 2.842522537782102 1106 3 1.3357956909099045 1106 4 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0.42284952940875087 1107 16 0.5120261718248429 1107 17 0.26493263183461124 1107 18 0.3727397073301759 1107 19 0.4528028737672187 1107 20 0.697916246030374 1107 21 0.5020603783292898 1107 22 0.4655689751824923 1107 23 0.6272063800267489 1107 24 0.5412138058444143 1107 25 0.7764981845602321 1108 1 0.8605266574002535 1108 2 3.2240645229320073 1108 3 2.08757478027076 1108 4 1.1732085582985832 1108 5 0.8651723032441438 1108 6 0.6587660990700215 1108 7 0.6361964785459548 1108 8 0.6754825072231512 1108 9 0.6127583932729692 1108 10 0.5045883847656327 1108 11 0.6957000418492427 1108 12 0.7672112510184377 1108 13 0.5707818539754257 1108 14 0.6791123003849151 1108 15 0.649951124670828 1108 16 0.4522913582483241 1108 17 0.6136041993064154 1108 18 0.5769375398312349 1108 19 0.5259101223390061 1108 20 0.5484786003219142 1108 21 0.6119439068137353 1108 22 0.7082028840865675 1108 23 0.728400385708639 1108 24 0.7941517885129983 1108 25 0.8010581717962353 1109 1 0.8898896385955872 1109 2 3.442893118381008 1109 3 1.980816141418586 1109 4 1.159416655497381 1109 5 0.9415412504766181 1109 6 0.7621047755011361 1109 7 0.6619253935679055 1109 8 0.5534167795236158 1109 9 0.7481765476505089 1109 10 0.5641591137059763 1109 11 0.6112016093267485 1109 12 0.8460900614574811 1109 13 0.8507496687750518 1109 14 0.8573405696770422 1109 15 0.834751404233856 1109 16 0.7164362600214247 1109 17 0.8159721285648089 1109 18 0.8141361359386181 1109 19 0.7953357013434186 1109 20 0.7396862923569003 1109 21 0.8639679532860214 1109 22 0.75098881578608 1109 23 0.8856531977987245 1109 24 0.7225870711907234 1109 25 0.754051066235867 1110 1 0.7651769892350515 1110 2 3.57926009171981 1110 3 2.03254370582221 1110 4 1.0701959504316108 1110 5 0.548155753217884 1110 6 0.35216887014269105 1110 7 0.35521634319688644 1110 8 0.24667707841771858 1110 9 0.33802003115596335 1110 10 0.18385913199754356 1110 11 0.22017584787845124 1110 12 0.01420291548583208 1110 13 0.22947496897922548 1110 14 0.3413693121564876 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-2.11797085784454 2315 23 -1.9817570841790513 2315 24 -1.9806533721500514 2315 25 -1.9143167992278478 2316 1 -1.4251003010422387 2316 2 -0.16203061003600894 2316 3 -0.3515127154435156 2316 4 -1.0845576554847867 2316 5 -1.2076059583577816 2316 6 -1.1720501540763308 2316 7 -1.215202490012647 2316 8 -1.198050161658358 2316 9 -1.1937985588021576 2316 10 -1.3220605297360706 2316 11 -1.9170563102839395 2316 12 -1.3968372699729628 2316 13 -1.279002097637381 2316 14 -1.5899595098700061 2316 15 -1.7606535838340847 2316 16 -1.436208679382439 2316 17 -1.5961260418530472 2316 18 -2.1730006381945515 2316 19 -2.010141346993109 2316 20 -2.0739560266735637 2316 21 -2.0401684933004773 2316 22 -1.834619017258703 2316 23 -1.8448191712995836 2316 24 -1.6846692574789657 2316 25 -1.5804021647781212 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127343.0 3 7.0 4 1082.0 5 5686.0 6 6638.0 7 6040.0 8 5481.0 9 5526.0 10 5154.0 11 5109.0 12 4894.0 13 4812.0 14 5002.0 15 5098.0 16 5435.0 17 5900.0 18 6697.0 19 8282.0 20 10828.0 21 13916.0 22 17104.0 23 20557.0 24 23941.0 25 27045.0 26 30273.0 27 34554.0 28 39484.0 29 47307.0 30 58650.0 31 73311.0 32 92522.0 33 119420.0 34 157206.0 35 214169.0 36 298258.0 37 374003.0 38 799097.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6817754013664 18.95048860936796 13.19022098550133 25.177515003764306 2 15.653642221510413 20.55805230048307 40.87091051879361 22.91739495921291 3 18.857084338804672 24.1438785879525 29.667559571480712 27.331477501762112 4 12.295565622877069 16.731668286549297 38.38401609104253 32.5887499995311 5 13.16294993943727 37.71533154202198 35.98172577331421 13.139992745226536 6 31.798598932788163 37.01525794078756 18.20937233314265 12.97677079328163 7 28.207691707457617 31.44138108000228 22.019159491361123 18.33176772117898 8 24.705054446437153 37.94385315498244 20.469264555780168 16.881827842800238 9 26.26483074133357 15.699044688129144 20.51649185563526 37.51963271490204 10 15.190258523510577 29.114440215852035 34.31360816891528 21.38169309172211 11 34.11999932477169 22.251286216151836 25.132410396265236 18.496304062811237 12 23.74378522829322 24.283617910546297 31.869634402245307 20.102962458915172 13 29.049028239224466 22.075367868405763 25.877596891926007 22.998007000443764 14 20.60523148010952 22.703757901406057 27.482694837579242 29.208315780905185 15 22.676081116920017 31.827673997338916 23.154393508065592 22.34185137767548 16 21.677405656997763 28.136367234081984 28.58065646321916 21.605570645701096 17 21.397471480728882 28.939365578212257 28.210023973780764 21.453138967278097 18 21.169480245174874 27.509129530156525 30.6852756266493 20.636114598019304 19 23.515017786812585 26.82408142732653 27.45835207422782 22.20254871163306 20 23.439370312671734 27.638023565634878 28.34635803995077 20.576248081742616 21 22.66900105752858 26.995114510575096 27.15582584203697 23.180058589859357 22 22.96058579257931 28.548985478976213 27.379538523289533 21.110890205154945 23 22.045016072345625 28.152094769257086 27.97743531954689 21.825453838850397 24 23.128227291348722 26.84089725639906 28.73086509790857 21.300010354343645 25 22.601400259373982 27.559517400828092 28.359960369876152 21.47912196992177 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 377.0 1 377.0 2 468.0 3 559.0 4 559.0 5 559.0 6 1347.5 7 2136.0 8 2136.0 9 2136.0 10 3122.0 11 4108.0 12 4108.0 13 4108.0 14 7368.0 15 10628.0 16 10628.0 17 10628.0 18 19749.5 19 28871.0 20 28871.0 21 28871.0 22 47836.0 23 66801.0 24 66801.0 25 66801.0 26 99381.0 27 131961.0 28 131961.0 29 131961.0 30 173159.0 31 214357.0 32 214357.0 33 214357.0 34 240697.0 35 267037.0 36 267037.0 37 267037.0 38 285007.0 39 302977.0 40 302977.0 41 302977.0 42 320954.5 43 338932.0 44 338932.0 45 338932.0 46 343393.5 47 347855.0 48 347855.0 49 347855.0 50 332721.0 51 317587.0 52 317587.0 53 317587.0 54 281454.0 55 245321.0 56 245321.0 57 245321.0 58 209560.0 59 173799.0 60 173799.0 61 173799.0 62 141650.5 63 109502.0 64 109502.0 65 109502.0 66 84475.5 67 59449.0 68 59449.0 69 59449.0 70 43282.0 71 27115.0 72 27115.0 73 27115.0 74 18960.0 75 10805.0 76 10805.0 77 10805.0 78 7411.5 79 4018.0 80 4018.0 81 4018.0 82 2589.5 83 1161.0 84 1161.0 85 1161.0 86 764.0 87 367.0 88 367.0 89 367.0 90 222.5 91 78.0 92 78.0 93 78.0 94 50.5 95 23.0 96 23.0 97 23.0 98 15.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.71763101261858 3 0.0 4 0.0 5 0.0 6 2.2507053147780188E-4 7 0.007764933335984165 8 0.0 9 0.0 10 0.004351363608570836 11 0.0024757758462558206 12 1.8755877623150154E-4 13 0.0 14 3.0009404197040246E-4 15 0.0 16 0.0 17 7.502351049260061E-5 18 0.011441085350121595 19 0.0034510814826596284 20 0.0 21 0.006827139454826656 22 0.0015379819650983126 23 0.0027008463777336224 24 0.010615826734702988 25 0.006827139454826656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2665831.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.29732150194684 #Duplication Level Percentage of deduplicated Percentage of total 1 85.23190466443779 62.47270318412579 2 10.373076976528091 15.206375162260446 3 2.3927187014862716 5.261396157796801 4 0.8328398091127589 2.441797089926317 5 0.37038421517426995 1.357408544943736 6 0.19873083179727108 0.873986260235634 7 0.12186169536445066 0.6252495104730311 8 0.08451948704381698 0.4956041612024228 9 0.05821686313079089 0.3840426120359174 >10 0.29613383087588635 3.7824733856882826 >50 0.020234692600326586 1.0391232052408383 >100 0.015957371334461564 2.419058561392133 >500 0.0015549368699482305 0.8234908038246435 >1k 0.0017104305568909919 2.1280224157784544 >5k 1.5549368699009015E-4 0.6892689450756687 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6657 0.24971575467462115 No Hit CTGTAGGACGTGGAATATGGCAAGA 6087 0.22833405418422997 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5399 0.20252596657477534 No Hit CTTTAGGACGTGAAATATGGCGAGG 4844 0.18170694241307872 No Hit GTATCAACGCAGAGTACTTTTTTTT 4467 0.16756501068522348 No Hit GGTATCAACGCAGAGTACTTTTTTT 2718 0.10195695075944425 No Hit TATCAACGCAGAGTACTTTTTTTTT 2709 0.10161934496222755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.751175524630031E-5 0.0 0.0 0.0 0.0 7 3.751175524630031E-5 0.0 0.0 0.0 0.0 8 3.751175524630031E-5 0.0 0.0 0.0 0.0 9 3.751175524630031E-5 0.0 0.0 0.0 0.0 10 3.751175524630031E-5 0.0 0.0 3.751175524630031E-5 0.0 11 3.751175524630031E-5 0.0 0.0 3.751175524630031E-5 0.0 12 3.751175524630031E-5 0.0 0.0 3.751175524630031E-5 0.0 13 7.502351049260061E-5 0.0 0.0 3.751175524630031E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 65 5.890635E-5 13.046619 5 TAGGACC 995 0.0 12.026782 4 CCAACGA 195 0.0 11.5983 19 GGCGAGG 1415 0.0 11.588055 19 TGTAGGA 3335 0.0 11.144295 2 CGGTAGG 120 6.0262937E-7 11.061376 1 GTCTTAA 235 0.0 10.862234 1 GTCCTAC 3230 0.0 10.653061 1 AGGACGT 3485 0.0 10.652762 5 TTAGGAC 2000 0.0 10.6474905 3 CTGTAGG 3360 0.0 10.635939 1 GTAGGAC 3480 0.0 10.586839 3 GGACGTG 3555 0.0 10.443004 6 GACGTGA 1760 0.0 10.386229 7 CTGCACG 100 2.6285566E-5 10.364231 9 TCCAACG 355 0.0 10.352708 18 TCGTACA 80 0.0024869286 10.210501 2 TAGGACG 3820 0.0 10.162549 4 TGGCGAG 3220 0.0 10.155266 18 GGTATCA 1670 0.0 10.14936 1 >>END_MODULE