FastQCFastQC Report
Thu 26 May 2016
SRR1512224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665831
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72660.2725604136196181No Hit
CTGTAGGACGTGGAATATGGCAAGA67470.2530918126467882No Hit
GTATCAACGCAGAGTACTTTTTTTT59980.22499550796730924No Hit
GTCCTAAAGTGTGTATTTCTCATTT58440.219218697659379No Hit
CTTTAGGACGTGAAATATGGCGAGG56370.21145376432339485No Hit
TATCAACGCAGAGTACTTTTTTTTT38910.1459582396633545No Hit
GGTATCAACGCAGAGTACTTTTTTT38100.14291978748840417No Hit
CTGAAGGACCTGGAATATGGCGAGA31850.11947494045946648No Hit
GTCCTACAGTGTGCATTTCTCATTT28000.10503291468964086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10250.012.7913174
AATACCG905.4043085E-712.6677295
TCCAACG3000.012.02960418
AGCGTAC600.005706928711.12727451
CTGTAGG35850.011.0940191
TGTAGGA35650.011.0864842
GGCGAGG16050.011.06572719
GTAGGAC35400.010.8963943
GGTATCA17150.010.7333471
GGACGTG36500.010.6158866
GACGTGA19750.010.5789997
AGGACGT37350.010.5564415
CGTGCGC1002.4083654E-510.4467610
AGGACCT23050.010.3869895
GGCGTGC1106.0717175E-610.3604258
TAGGACA10150.010.2964324
TTAGGAC23000.010.28564553
GCCGGTT2500.010.25681911
TAGGACG40250.010.1971294
GCGTGCG750.00265545410.1301939
Produced by FastQC (version 0.11.5)