##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512224_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2665831 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.03712800999013 31.0 31.0 34.0 28.0 34.0 2 31.23893712692215 31.0 31.0 34.0 28.0 34.0 3 31.33147074964617 33.0 31.0 34.0 28.0 34.0 4 34.91961943574068 37.0 35.0 37.0 32.0 37.0 5 34.55306094047222 37.0 35.0 37.0 31.0 37.0 6 34.48968257927828 37.0 35.0 37.0 30.0 37.0 7 34.33103036163958 37.0 35.0 37.0 30.0 37.0 8 34.238842222181376 37.0 35.0 37.0 30.0 37.0 9 35.76445018457659 39.0 35.0 39.0 30.0 39.0 10 35.58214080337426 39.0 35.0 39.0 30.0 39.0 11 35.562739723560874 39.0 35.0 39.0 30.0 39.0 12 35.401587347435004 39.0 35.0 39.0 30.0 39.0 13 35.38262102886492 39.0 35.0 39.0 30.0 39.0 14 36.43136792992504 40.0 36.0 41.0 30.0 41.0 15 36.372240400835615 40.0 36.0 41.0 29.0 41.0 16 36.28367552181665 40.0 36.0 41.0 29.0 41.0 17 36.22127321649422 39.0 36.0 41.0 29.0 41.0 18 36.15633549163469 39.0 36.0 41.0 29.0 41.0 19 36.182125573601624 40.0 36.0 41.0 29.0 41.0 20 36.06476854684337 39.0 36.0 41.0 28.0 41.0 21 35.958617031612285 39.0 36.0 41.0 27.0 41.0 22 36.052999608752394 39.0 36.0 41.0 29.0 41.0 23 35.94684696816865 39.0 36.0 41.0 28.0 41.0 24 35.838386979519704 39.0 36.0 41.0 28.0 41.0 25 35.411977353403124 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28578973814518704 1101 2 0.2566243628515821 1101 3 0.34018281773600023 1101 4 0.2831520119866653 1101 5 0.27619517022582585 1101 6 0.38483003523355563 1101 7 0.23092200709093902 1101 8 0.2520039748319647 1101 9 0.19069800903017153 1101 10 0.29883529464673586 1101 11 0.40075046236358247 1101 12 0.329419439925843 1101 13 0.09670929675377948 1101 14 0.14906056528795375 1101 15 0.16279255271395243 1101 16 0.12812265717798965 1101 17 0.10910256358824455 1101 18 0.16667310769321375 1101 19 0.18038676876486903 1101 20 0.21930594256702562 1101 21 0.15199635279022772 1101 22 0.0918362421797454 1101 23 0.06315708381417551 1101 24 0.03651006306839122 1101 25 0.0960836022768703 1102 1 0.43428971233674574 1102 2 0.4813155926205397 1102 3 0.4383274194921505 1102 4 0.36816224062332026 1102 5 0.5749453556219066 1102 6 0.5735794169743826 1102 7 0.6032852174519476 1102 8 0.5821491489474724 1102 9 0.8600853427699064 1102 10 0.752346941440905 1102 11 0.7396064754363749 1102 12 0.6984417639008385 1102 13 0.7691457834866071 1102 14 0.8741508458289786 1102 15 0.9762930790101336 1102 16 0.9175086253571649 1102 17 0.8929655253388233 1102 18 1.0658658261845346 1102 19 0.9041284765780375 1102 20 0.895722809657471 1102 21 0.9009679354095823 1102 22 0.9319171761447649 1102 23 0.8420217018786502 1102 24 0.9966420650781842 1102 25 0.9800674716743956 1103 1 0.17476015971676873 1103 2 0.19076380113740043 1103 3 0.19888161304094254 1103 4 0.16536878879226435 1103 5 0.13959588642824627 1103 6 0.2378542095218279 1103 7 0.2149781343686996 1103 8 0.26644465695926556 1103 9 0.3842700045458969 1103 10 0.4743098806410444 1103 11 0.3387352719387664 1103 12 0.42993425572095845 1103 13 0.431523696775173 1103 14 0.4301699331569182 1103 15 0.4178236338496717 1103 16 0.526861723363325 1103 17 0.21738158192471957 1103 18 0.36593760592703006 1103 19 0.510562719874045 1103 20 0.23786585987945585 1103 21 0.4616371835128632 1103 22 0.5950269982100238 1103 23 0.5763424221722175 1103 24 0.3228455595506077 1103 25 0.5041702876103145 1104 1 0.30939936919275723 1104 2 0.2521664803306827 1104 3 0.32008794252906014 1104 4 0.2791756246369914 1104 5 0.3797601731863054 1104 6 0.34762968652299975 1104 7 0.32480196510074677 1104 8 0.26187256002117465 1104 9 0.44697564137063495 1104 10 0.45091308138346875 1104 11 0.548050641072976 1104 12 0.6407888598655944 1104 13 0.7019828924802667 1104 14 0.7620147240872868 1104 15 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1107 12 0.13409755499407083 1107 13 0.2288816925951238 1107 14 0.25190626689217055 1107 15 0.2904480480099991 1107 16 0.33086755448814387 1107 17 0.30427538236147456 1107 18 0.42145641312750826 1107 19 0.4228825914457772 1107 20 0.3145670555320379 1107 21 0.25626277998640745 1107 22 0.3747904892690386 1107 23 0.27846966002406504 1107 24 0.3451383559760046 1107 25 0.14701403736616925 1108 1 -0.04987874794310798 1108 2 0.0854827969410401 1108 3 0.005136714967772349 1108 4 0.13066161694487022 1108 5 0.08349233108253173 1108 6 0.11962624189192894 1108 7 0.17465295718964313 1108 8 0.14319737606621175 1108 9 0.19471764776501743 1108 10 0.219885123465545 1108 11 0.16425923708453638 1108 12 0.307735605054674 1108 13 0.3138170748364928 1108 14 0.40851294751350764 1108 15 0.26940173325829164 1108 16 0.3336763492663266 1108 17 0.3913194443621393 1108 18 0.32816132417242017 1108 19 0.422670651865225 1108 20 0.4615423919563568 1108 21 0.43189163329802227 1108 22 0.3143150778323971 1108 23 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-0.7401859927622425 2314 21 -0.7822158069169944 2314 22 -0.5232003607805495 2314 23 -0.5582373298239531 2314 24 -0.5574765575200118 2314 25 -0.757728765894754 2315 1 -0.328357088153556 2315 2 -0.3613906066296728 2315 3 -0.42787500097203335 2315 4 -0.5087959587615245 2315 5 -0.5433595268888425 2315 6 -0.66085758679192 2315 7 -0.7252172845484566 2315 8 -0.6287300200127461 2315 9 -0.6513346143882899 2315 10 -0.6606234525495225 2315 11 -0.634882023178811 2315 12 -0.5555237628872263 2315 13 -0.6313830077035689 2315 14 -0.9005130479546182 2315 15 -0.8249395728280078 2315 16 -1.0458627726905263 2315 17 -0.9486107294884789 2315 18 -0.8519549417759649 2315 19 -1.0972837503814645 2315 20 -0.9372052853805428 2315 21 -0.9552392404296342 2315 22 -0.7871924287356862 2315 23 -1.0050330055626446 2315 24 -0.8564138605208313 2315 25 -0.9612318748415305 2316 1 -0.8884990248338767 2316 2 -0.8648956013262712 2316 3 -0.8451672580049774 2316 4 -0.8260312240107837 2316 5 -0.9450075393003843 2316 6 -0.8966635795232065 2316 7 -0.8987023095049764 2316 8 -0.85570500016739 2316 9 -1.0837229093614766 2316 10 -1.012154428262427 2316 11 -1.1510016516466592 2316 12 -1.2457625629840265 2316 13 -1.306693126685225 2316 14 -1.4772570078432565 2316 15 -1.3244214451437983 2316 16 -1.2830548870178546 2316 17 -1.4235983473449991 2316 18 -1.352706238225963 2316 19 -1.4277797824416112 2316 20 -1.3853400483331697 2316 21 -1.282339964360986 2316 22 -1.2528695793274238 2316 23 -1.1458610599943242 2316 24 -1.2030084883667413 2316 25 -1.3231758374440616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35276.0 3 1.0 4 670.0 5 5909.0 6 7520.0 7 6076.0 8 4960.0 9 4415.0 10 4328.0 11 4100.0 12 3759.0 13 3658.0 14 3722.0 15 3602.0 16 3732.0 17 3973.0 18 4237.0 19 4821.0 20 5901.0 21 7574.0 22 9712.0 23 12626.0 24 15366.0 25 18728.0 26 22175.0 27 25076.0 28 30029.0 29 36578.0 30 46337.0 31 60890.0 32 79347.0 33 103243.0 34 135584.0 35 180269.0 36 259446.0 37 447533.0 38 1064658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.492008429707106 19.673021094611183 13.831975473379893 25.00299500230182 2 14.520275291269403 20.407520206644758 41.524537752018034 23.5476667500678 3 18.747887619282693 24.464641607063616 30.150148302724368 26.637322470929327 4 12.589357689966093 17.281328036173335 38.09131186485565 32.038002409004925 5 13.07527604769792 37.882945119173506 35.97153104499992 13.070247788128656 6 31.795638958358573 37.345465635293465 17.986699081824767 12.872196324523197 7 28.122675443417084 31.60823022914806 22.047759216544485 18.221335110890376 8 24.771150159181133 38.101515062282644 20.3457383457541 16.781596432782123 9 26.130501145796565 15.638913344469325 20.492746914564353 37.73783859516976 10 15.128265820301436 29.304220710165048 34.25648512602637 21.311028343507147 11 34.207344726653716 22.291398066869206 24.99667083172189 18.50458637475519 12 23.628767164910304 24.457964514629772 31.818783711345546 20.09448460911438 13 29.05881880734375 22.085345995301278 25.914133341535905 22.941701855819066 14 20.567545354525475 22.73006053271944 27.544731830337334 29.15766228241775 15 22.559757163901235 31.908286759363214 23.215650204382797 22.31630587235275 16 21.648971746521067 28.341856629321217 28.449440343367606 21.559731280790118 17 21.313504119353404 29.057168290112916 28.224069717847833 21.405257872685855 18 21.16582033894872 27.54398159523241 30.680339451375577 20.6098586144433 19 23.446647593189514 26.874846905148903 27.62808295049461 22.050422551166974 20 23.404671939068905 27.69084011702167 28.370515610329388 20.533972333580035 21 22.632680016100046 26.968251175712187 27.25615389722754 23.142914910960222 22 22.90782874083166 28.502181871243902 27.53561647381248 21.05437291411196 23 22.00206989865449 28.12969014164814 28.057292454022782 21.81094750567459 24 23.130536031728944 26.856766239120184 28.780969236234405 21.231728492916467 25 22.520925619140225 27.577062400211283 28.449913566463785 21.452098414184704 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 384.0 1 384.0 2 458.0 3 532.0 4 532.0 5 532.0 6 1369.0 7 2206.0 8 2206.0 9 2206.0 10 3291.0 11 4376.0 12 4376.0 13 4376.0 14 7541.5 15 10707.0 16 10707.0 17 10707.0 18 19928.0 19 29149.0 20 29149.0 21 29149.0 22 48765.0 23 68381.0 24 68381.0 25 68381.0 26 100648.0 27 132915.0 28 132915.0 29 132915.0 30 174488.0 31 216061.0 32 216061.0 33 216061.0 34 242283.0 35 268505.0 36 268505.0 37 268505.0 38 285529.5 39 302554.0 40 302554.0 41 302554.0 42 320494.0 43 338434.0 44 338434.0 45 338434.0 46 343218.0 47 348002.0 48 348002.0 49 348002.0 50 332982.5 51 317963.0 52 317963.0 53 317963.0 54 280815.5 55 243668.0 56 243668.0 57 243668.0 58 208264.0 59 172860.0 60 172860.0 61 172860.0 62 140732.0 63 108604.0 64 108604.0 65 108604.0 66 83264.5 67 57925.0 68 57925.0 69 57925.0 70 42394.0 71 26863.0 72 26863.0 73 26863.0 74 18674.0 75 10485.0 76 10485.0 77 10485.0 78 7113.5 79 3742.0 80 3742.0 81 3742.0 82 2436.0 83 1130.0 84 1130.0 85 1130.0 86 724.0 87 318.0 88 318.0 89 318.0 90 184.5 91 51.0 92 51.0 93 51.0 94 32.5 95 14.0 96 14.0 97 14.0 98 8.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4279716156050402 2 0.0 3 0.0 4 0.0 5 0.03349799743494617 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007464839294013761 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2665831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.87802756623165 #Duplication Level Percentage of deduplicated Percentage of total 1 83.14388093495884 58.099304039365364 2 11.628963490560222 16.25218062720137 3 2.829334233982307 5.931248867888957 4 0.9835511659319295 2.749144619431626 5 0.4461814132793026 1.5589138548335653 6 0.25760037788588275 1.0800363784188842 7 0.14904451003836433 0.7290455466739418 8 0.10114918027161375 0.5654484165857248 9 0.07237314599072314 0.45515634215351936 >10 0.3420074290843994 4.187689990238367 >50 0.023240838145144806 1.145773065337837 >100 0.01811623804406589 2.59987372830846 >500 0.0021981025285596595 1.0299379529726347 >1k 0.0020908780148118263 2.4364558673636387 >5k 2.6806128395023414E-4 1.1797907032261472 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7266 0.2725604136196181 No Hit CTGTAGGACGTGGAATATGGCAAGA 6747 0.2530918126467882 No Hit GTATCAACGCAGAGTACTTTTTTTT 5998 0.22499550796730924 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5844 0.219218697659379 No Hit CTTTAGGACGTGAAATATGGCGAGG 5637 0.21145376432339485 No Hit TATCAACGCAGAGTACTTTTTTTTT 3891 0.1459582396633545 No Hit GGTATCAACGCAGAGTACTTTTTTT 3810 0.14291978748840417 No Hit CTGAAGGACCTGGAATATGGCGAGA 3185 0.11947494045946648 No Hit GTCCTACAGTGTGCATTTCTCATTT 2800 0.10503291468964086 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1025 0.0 12.791317 4 AATACCG 90 5.4043085E-7 12.667729 5 TCCAACG 300 0.0 12.029604 18 AGCGTAC 60 0.0057069287 11.1272745 1 CTGTAGG 3585 0.0 11.094019 1 TGTAGGA 3565 0.0 11.086484 2 GGCGAGG 1605 0.0 11.065727 19 GTAGGAC 3540 0.0 10.896394 3 GGTATCA 1715 0.0 10.733347 1 GGACGTG 3650 0.0 10.615886 6 GACGTGA 1975 0.0 10.578999 7 AGGACGT 3735 0.0 10.556441 5 CGTGCGC 100 2.4083654E-5 10.44676 10 AGGACCT 2305 0.0 10.386989 5 GGCGTGC 110 6.0717175E-6 10.360425 8 TAGGACA 1015 0.0 10.296432 4 TTAGGAC 2300 0.0 10.2856455 3 GCCGGTT 250 0.0 10.256819 11 TAGGACG 4025 0.0 10.197129 4 GCGTGCG 75 0.002655454 10.130193 9 >>END_MODULE