FastQCFastQC Report
Thu 26 May 2016
SRR1512223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2967798
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80950.2727611515338982No Hit
CTGTAGGACGTGGAATATGGCAAGA68260.23000217669800976No Hit
GTCCTAAAGTGTGTATTTCTCATTT56620.1907811785033887No Hit
GTATCAACGCAGAGTACTTTTTTTT49750.16763270276481082No Hit
CTTTAGGACGTGAAATATGGCGAGG49370.16635229217082834No Hit
GTCCTACAGTGTGCATTTCTCATTT37820.12743454911688734No Hit
GGTATCAACGCAGAGTACTTTTTTT31140.10492627867530067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG350.002294636816.1510079
TCCAACG4050.014.19124518
TAGGACC12650.012.8850644
CGGTAGG1308.240022E-1012.5821741
CGCGCCT604.3910043E-412.56189412
GCCGGTT2750.011.99089911
TGTAGGA37500.011.8314372
CTGTAGG37550.011.6251081
CGTGCGC658.606233E-411.59559510
CCAACGA3200.011.4831319
GTAGGAC40700.011.2505273
GTCCTAC38400.011.128181
CGGTTTC3100.010.94100513
GTCTACG750.001438767510.9045511
TAGGACT4850.010.8787094
AGGACGT37750.010.8069215
GGACGTG37200.010.789416
TAAGACG700.001598654210.76774
GGCGAGG16700.010.71970519
TCCTACA42200.010.5379432
Produced by FastQC (version 0.11.5)