FastQCFastQC Report
Thu 26 May 2016
SRR1512223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2967798
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT94100.31707009708881806No Hit
CTGTAGGACGTGGAATATGGCAAGA77000.25945162035960667No Hit
GTATCAACGCAGAGTACTTTTTTTT63640.21443507947643334No Hit
GTCCTAAAGTGTGTATTTCTCATTT61160.20607871559991617No Hit
CTTTAGGACGTGAAATATGGCGAGG57360.19327460966009144No Hit
GTCCTACAGTGTGCATTTCTCATTT44210.1489656641051716No Hit
GGTATCAACGCAGAGTACTTTTTTT42420.1429342563072015No Hit
TATCAACGCAGAGTACTTTTTTTTT39600.1334322618992263No Hit
CTGAAGGACCTGGAATATGGCGAGA32180.10843056030093692No Hit
CTGTAGGACCTGGAATATGGCGAGA31440.10593712914423421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12500.013.7556464
CCAACGA2600.012.42003619
TCCAACG3800.011.99645618
GGCGAGG16650.011.75087619
AGGACCG1157.0846E-811.5649195
TTAGGAC21650.011.0574533
TGGACCG951.3616274E-510.9997175
GATATAC10850.010.9907241
TGTAGGA42600.010.8823492
ACCGTCT700.001495775110.8539378
AGGACGT40050.010.8162015
AGGACCT24550.010.757365
GACGTGA20350.010.6872617
GGACGTG39200.010.6843446
TATACCG909.5233365E-510.5552845
GTCCTAC40150.010.526021
GGACCTG24950.010.5059166
ACTGTTC12700.010.4693498
CTGTAGG43200.010.445361
GTAGGAC42150.010.2998533
Produced by FastQC (version 0.11.5)