FastQCFastQC Report
Thu 26 May 2016
SRR1512222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122248
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71890.23025076803636352No Hit
CTGTAGGACGTGGAATATGGCAAGA62250.19937557810910603No Hit
GTCCTAAAGTGTGTATTTCTCATTT50370.16132607019045253No Hit
CTTTAGGACGTGAAATATGGCGAGG46570.1491553521693344No Hit
GTATCAACGCAGAGTACTTTTTTTT45850.1468493213863857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA400.00557407914.1342957
TCCAACG4550.013.25498718
TAGGACC11350.012.7021864
TGTAGGA35200.011.6021912
AGGACGT35750.011.0965765
CCGGTTT3150.011.06830312
CTGTAGG35000.011.0558941
GGACGTG34900.011.0428416
ACTGTTC12200.010.8896628
GGTATCA16700.010.7601651
GTAGGAC37900.010.6908483
GACGTGA16450.010.6544237
CCAACGA3450.010.65261619
CCAACGT1951.8189894E-1210.63160419
GATATAC11000.010.5811991
TTAGGAC18500.010.5434213
TAGGACG38200.010.5328874
GTCCTAC32300.010.4944161
GACGTGG20150.010.4282817
GGTCTAC2650.010.402571
Produced by FastQC (version 0.11.5)