FastQCFastQC Report
Thu 26 May 2016
SRR1512222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122248
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78990.2529907938126632No Hit
CTGTAGGACGTGGAATATGGCAAGA72920.23354967318419292No Hit
GTATCAACGCAGAGTACTTTTTTTT61830.19803039348571927No Hit
GTCCTAAAGTGTGTATTTCTCATTT54540.17468183180836372No Hit
CTTTAGGACGTGAAATATGGCGAGG54080.17320853436370204No Hit
TATCAACGCAGAGTACTTTTTTTTT39370.12609504433984745No Hit
GGTATCAACGCAGAGTACTTTTTTT38070.12193137764841229No Hit
GTCCTACAGTGTGCATTTCTCATTT31770.10175360829761122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3300.013.23838318
GCCGGTT2150.011.92659211
CGAAATC2400.011.87137613
CCGGTTT2100.011.75831612
TAGGACC11150.011.5879914
CCAACGA2300.011.56168819
GGTATCA19250.011.2980911
ACGAAAT2400.011.07995112
AGGACGT32750.011.052435
GGACGTG31850.010.8240126
GTCCTAA18150.010.7214741
TGTAGGA36100.010.6583982
GATATAC11100.010.5701961
GTAGGAC34450.010.5621823
GTCCTAG4350.010.5257451
GACGTGG17500.010.4739477
GCCTCGA1659.349606E-1010.36047416
GTCCTAC34850.010.3190451
TAGGACG36000.010.2657634
AATCCCG4350.010.26123519
Produced by FastQC (version 0.11.5)