FastQCFastQC Report
Thu 26 May 2016
SRR1512221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103222
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72140.23246806061570846No Hit
CTGTAGGACGTGGAATATGGCAAGA64560.20804183522803074No Hit
GTCCTAAAGTGTGTATTTCTCATTT51940.16737442567756997No Hit
CTTTAGGACGTGAAATATGGCGAGG48890.15754593129334607No Hit
GTATCAACGCAGAGTACTTTTTTTT48270.15554800784474976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10550.012.7701374
TCCAACG5100.012.74711518
CCAACGA3500.012.651919
ATACCGC550.003266823211.9907776
TAGAACG951.1507946E-611.9006214
CGCTACA708.060114E-411.6865782
TATACCG908.1910875E-611.5149525
ATACCGA752.2416413E-411.3055916
TCGAATA600.006256080710.99083711
CGTTAGG750.001435589710.9074721
TGTAGGA34250.010.8676642
CTGTAGG34600.010.7281771
GTCCTAA17600.010.6324621
GTAGGAC36450.010.5198343
GGACGTG39500.010.4230866
AGGACGT40150.010.41865
GGCGAGG16550.010.41787319
GTCCTAC36950.010.405641
GCCGTGC1002.6325048E-510.3629578
CCAACGT2200.010.2781419
Produced by FastQC (version 0.11.5)