FastQCFastQC Report
Thu 26 May 2016
SRR1512221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103222
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT81760.2634680986407031No Hit
CTGTAGGACGTGGAATATGGCAAGA73190.23585164064962158No Hit
GTATCAACGCAGAGTACTTTTTTTT69350.22347740509702493No Hit
GTCCTAAAGTGTGTATTTCTCATTT61200.19721437911950868No Hit
CTTTAGGACGTGAAATATGGCGAGG59310.19112393505846503No Hit
TATCAACGCAGAGTACTTTTTTTTT42900.1382434128141654No Hit
GGTATCAACGCAGAGTACTTTTTTT42470.1368577562288486No Hit
GTCCTACAGTGTGCATTTCTCATTT34400.11085252682534476No Hit
TTGTAGAACAGTGTATATCAATGAG33890.10920907366601551No Hit
CTGAAGGACCTGGAATATGGCGAGA32990.1063088622083757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4400.013.38225418
TAGGACC10150.013.1974584
CCAACGA3000.012.34700519
ACTGTTC15350.011.0129048
GGCGAGG16450.010.79677319
GTAGGAC38200.010.7189233
TGTAGGA40400.010.6055312
CGTTTCC3150.010.55231514
GATATAC14550.010.489841
TATTGCG1002.4086337E-510.44679316
GTCCTAC37550.010.4410391
CCAACGT2200.010.36112219
AGGACGT39150.010.2889565
CTGTAGG40450.010.2348861
ACCTTTT10150.010.19883815
GACGTGG21150.010.1930897
TGTAGAA16750.010.1525572
AGAACAG12450.010.0726055
ATAGGAC4350.010.0462923
GGACGTG38600.010.0137646
Produced by FastQC (version 0.11.5)