FastQCFastQC Report
Thu 26 May 2016
SRR1512220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3144544
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68610.21818743830584023No Hit
CTGTAGGACGTGGAATATGGCAAGA59540.18934382854874984No Hit
GTCCTAAAGTGTGTATTTCTCATTT49890.15865575422064374No Hit
GTATCAACGCAGAGTACTTTTTTTT47270.1503238625377797No Hit
CTTTAGGACGTGAAATATGGCGAGG46700.1485111990800574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10750.012.5335474
TCCAACG3600.011.77833918
CCAACGA2500.011.68411319
AAGGCGT1302.9813236E-911.5963966
GTACTAG1454.640242E-911.2737881
CCGTGCG600.006250902510.9920689
GTCCTAA17150.010.9615411
GGCGAGG16100.010.944344519
CGCATAT750.001446050110.8979961
GGATCGT700.001598227510.7680836
TGATCGA700.001598651310.7677410
ATAGGAC4400.010.7069013
TGTAGGA34900.010.6559922
GTCCTAC33900.010.6086671
CTGTAGG33950.010.472671
GTAGGAC35650.010.4131743
CCAACGT1554.1945896E-910.33454319
AGGACCT23300.010.2712725
AGGACGT37100.010.2601545
TAGGACA11050.010.2321154
Produced by FastQC (version 0.11.5)