FastQCFastQC Report
Thu 26 May 2016
SRR1512220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3144544
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76350.24280149999491182No Hit
CTGTAGGACGTGGAATATGGCAAGA66860.21262224347949973No Hit
GTATCAACGCAGAGTACTTTTTTTT63560.20212787609268623No Hit
GTCCTAAAGTGTGTATTTCTCATTT56670.18021690903355145No Hit
CTTTAGGACGTGAAATATGGCGAGG53740.17089918283859282No Hit
TATCAACGCAGAGTACTTTTTTTTT41540.13210182462067632No Hit
GGTATCAACGCAGAGTACTTTTTTT40160.1277132709861907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11600.012.7767074
TCCAACG3750.012.40941718
ACGGTGC550.003076908612.0870958
TACACCG1353.7107384E-1011.9637555
GGTATCA19550.011.0763481
GAACAGT8300.010.9844866
TGTAGGA37250.010.4825722
GTAGGAC35800.010.4029233
GTCCTAC33900.010.35537051
GGACGTG35700.010.3482746
AGGACGT36550.010.2674375
CCAACGA3250.010.22786619
CCTACAC4950.010.172393
TAGGACT4950.010.172394
TAGACGA750.002647407410.1340045
CGCACTG750.002647407410.1340045
CTGTAGG36100.010.1206661
GTATTAG3300.010.1174631
CTAGGAC4700.010.1070523
ATAGGAC5000.010.0706663
Produced by FastQC (version 0.11.5)