FastQCFastQC Report
Thu 26 May 2016
SRR1512219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245564
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58140.25891045634860554No Hit
GTATCAACGCAGAGTACTTTTTTTT53720.2392272052811677No Hit
CTGTAGGACGTGGAATATGGCAAGA52030.2317012563436179No Hit
GTCCTAAAGTGTGTATTTCTCATTT43520.19380431820246494No Hit
CTTTAGGACGTGAAATATGGCGAGG40200.17901961378076955No Hit
GGTATCAACGCAGAGTACTTTTTTT33510.14922754372620864No Hit
TATCAACGCAGAGTACTTTTTTTTT31030.13818354765217114No Hit
GTCCTACAGTGTGCATTTCTCATTT25540.1137353466656929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG400.005578771214.1322564
TAGGACC8300.013.0538924
TGTAGGA28950.011.9724882
GGCCTAC1506.311893E-1011.5875331
TGTAGAA8900.011.3730912
CTGTAGG29400.011.3371421
GTAGGAC30050.011.3183463
GTCCTAC24800.011.090061
AGGAGCG855.7944693E-511.0841235
CGCTGTC600.006251285310.9917557
GACGTGG16750.010.9120717
GGTATCA13700.010.8959631
CGTGGAA16900.010.7032449
GGACGTG31350.010.6085846
AGGACGT31800.010.5769725
GGCGAGG12600.010.54358219
TCCTACA27450.010.5408942
GGTTTAC1954.0017767E-1110.4864551
TAGGACG33100.010.44624
AGGACCT17150.010.4378195
Produced by FastQC (version 0.11.5)