##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512219_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2245564 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.98138017887711 31.0 29.0 34.0 23.0 34.0 2 25.999453144065367 31.0 25.0 34.0 2.0 34.0 3 27.712883266742786 31.0 26.0 34.0 16.0 34.0 4 32.24456884773714 35.0 32.0 37.0 23.0 37.0 5 32.67056561291506 35.0 33.0 37.0 26.0 37.0 6 32.88752669707922 35.0 33.0 37.0 26.0 37.0 7 32.8749401041342 36.0 33.0 37.0 26.0 37.0 8 32.87586103090359 36.0 33.0 37.0 26.0 37.0 9 34.34223651608237 38.0 35.0 39.0 27.0 39.0 10 34.19514117611433 38.0 34.0 39.0 26.0 39.0 11 34.2039950765153 38.0 35.0 39.0 26.0 39.0 12 34.076606144380655 38.0 34.0 39.0 25.0 39.0 13 34.07874413733031 38.0 34.0 39.0 25.0 39.0 14 35.07970826037467 39.0 35.0 41.0 25.0 41.0 15 35.00222794807897 39.0 34.0 41.0 25.0 41.0 16 34.94699817061549 39.0 34.0 41.0 25.0 41.0 17 34.927251238441656 39.0 34.0 41.0 25.0 41.0 18 34.868979908833595 39.0 34.0 41.0 24.0 41.0 19 34.88067674757878 39.0 34.0 41.0 24.0 41.0 20 34.79146575203379 39.0 34.0 41.0 23.0 41.0 21 34.700603946269176 39.0 34.0 41.0 22.0 41.0 22 34.59142424798402 39.0 34.0 41.0 22.0 41.0 23 34.45829021127877 39.0 34.0 41.0 19.0 41.0 24 34.33154744197894 39.0 34.0 41.0 18.0 41.0 25 34.18701849513084 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3542131108887041 1101 2 3.362695648655965 1101 3 1.9411693915987733 1101 4 0.9309329976577345 1101 5 0.5177134655562412 1101 6 0.4227739169055411 1101 7 0.3899610178727073 1101 8 0.3364390890891116 1101 9 0.24165817133084033 1101 10 0.10400832811383509 1101 11 0.30292575705002633 1101 12 0.20666457866635568 1101 13 0.32999154095703176 1101 14 0.22805975002326306 1101 15 0.4909944990340449 1101 16 0.5109177567177099 1101 17 0.3583036469719616 1101 18 0.3654431794982713 1101 19 0.22151542343534913 1101 20 0.1907303491625214 1101 21 0.2967674018637041 1101 22 0.24224285599260753 1101 23 0.29689229201402156 1101 24 0.3793601578753183 1101 25 0.3756952741489954 1102 1 0.9629962938558627 1102 2 3.9282451667277414 1102 3 2.3149788440432424 1102 4 1.4675554037163394 1102 5 0.9706760431478969 1102 6 0.91058976080447 1102 7 1.0300720333546423 1102 8 0.8204497481739566 1102 9 0.798515425517472 1102 10 0.8600939833092838 1102 11 0.9294281699025859 1102 12 1.0357131540057338 1102 13 1.0279075385762937 1102 14 1.12284910178591 1102 15 1.0467020621600014 1102 16 0.8033560580580854 1102 17 1.0539523767453147 1102 18 1.1682208502284652 1102 19 1.0348437041226788 1102 20 0.8563194034756094 1102 21 1.0535567165165176 1102 22 1.0582458989986137 1102 23 1.134935005427657 1102 24 1.1112516316065566 1102 25 0.9739528249288014 1103 1 1.0588413059312423 1103 2 3.88985830635135 1103 3 2.473696411137773 1103 4 1.596559229055245 1103 5 1.2930420618489933 1103 6 0.9529505413851425 1103 7 0.8347656914514445 1103 8 0.8520698635637061 1103 9 0.8437924289096586 1103 10 1.0211510763035463 1103 11 0.9201204328461614 1103 12 0.9474961547939742 1103 13 0.9031974609866324 1103 14 1.0513109831273724 1103 15 1.051136836228288 1103 16 1.0416955738845104 1103 17 1.053219543551748 1103 18 0.966669057053636 1103 19 1.1671540929775688 1103 20 1.1480273077489613 1103 21 1.3209149158118905 1103 22 1.289055616719331 1103 23 1.2840577461734313 1103 24 1.3904207851212576 1103 25 1.3102829675416672 1104 1 1.3484992281392856 1104 2 4.004795310351053 1104 3 2.6121107997117825 1104 4 1.6238099441172196 1104 5 1.3419540424746756 1104 6 1.099912633054167 1104 7 0.9423620919861619 1104 8 0.8586766522545162 1104 9 1.1037075233924938 1104 10 0.9722419978963259 1104 11 0.8747538469355831 1104 12 0.9020096849364094 1104 13 1.01605692306714 1104 14 1.3982843711236157 1104 15 1.005870265230648 1104 16 1.1497192702749572 1104 17 1.1527797011823964 1104 18 1.1412424086158808 1104 19 1.1166311578613488 1104 20 1.2702830369514402 1104 21 1.1531673588100873 1104 22 1.3005995254985834 1104 23 1.2493871747578709 1104 24 1.394923018936474 1104 25 1.1946242041367654 1105 1 1.0463440127694028 1105 2 3.7096441116370755 1105 3 2.4790359997863085 1105 4 1.5861190229727953 1105 5 1.157050732524894 1105 6 0.9307451250706862 1105 7 0.6520094321973531 1105 8 0.5544684794213239 1105 9 0.9439473095174833 1105 10 0.7398496395083569 1105 11 0.6463339482402404 1105 12 0.4781742768363699 1105 13 0.6575660504518979 1105 14 0.7460710940610227 1105 15 0.7198765653576018 1105 16 0.5560458282998226 1105 17 0.5712227378955888 1105 18 0.6912192272684123 1105 19 0.7271518065924454 1105 20 0.8243969678580214 1105 21 0.6957965569087818 1105 22 0.8485898976567015 1105 23 0.8025896670776973 1105 24 0.8295375369594353 1105 25 0.8007037833194843 1106 1 0.1192365151518473 1106 2 2.7799579933476295 1106 3 1.2161276573667763 1106 4 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1107 16 0.9529207305585388 1107 17 0.7432701945035021 1107 18 0.833166505999813 1107 19 0.7645920393756 1107 20 1.0492667890835463 1107 21 1.1103721403044204 1107 22 0.9827376491784392 1107 23 1.218505529365153 1107 24 1.18862210347244 1107 25 1.3323114218195116 1108 1 0.8760295165857954 1108 2 3.3590535249175595 1108 3 2.0603477504544365 1108 4 1.1954437129801292 1108 5 0.846297249824211 1108 6 0.5917671908071895 1108 7 0.7732525005846611 1108 8 0.5938890700968287 1108 9 0.7365230726237897 1108 10 0.5494227379947247 1108 11 0.5538009019216261 1108 12 0.6180185825307305 1108 13 0.6507540546308377 1108 14 0.5837046826756662 1108 15 0.736134989098268 1108 16 0.73483146796152 1108 17 0.7927703483697783 1108 18 0.7135471836795304 1108 19 0.7596280090009557 1108 20 0.622194833597085 1108 21 0.6982409831572127 1108 22 0.6945314575396182 1108 23 0.7440453723338365 1108 24 0.7747988837911421 1108 25 0.5920446070530474 1109 1 0.7301503238767602 1109 2 3.3399265426796383 1109 3 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-1.7330776544079995 2315 21 -1.609877727327401 2315 22 -1.474149608908732 2315 23 -1.5741917807766086 2315 24 -1.5623703290487256 2315 25 -1.4551299200913164 2316 1 -0.892609634191011 2316 2 0.4615125992077722 2316 3 -3.997260597365937E-4 2316 4 -0.7078190759888479 2316 5 -0.779410688434897 2316 6 -0.7560172542277428 2316 7 -1.0203951430967848 2316 8 -1.0044247940234214 2316 9 -0.9590268135208291 2316 10 -0.9654675942207973 2316 11 -1.471164369666326 2316 12 -1.036361738147896 2316 13 -0.9902799607135648 2316 14 -1.1665573360457273 2316 15 -1.5317455182560806 2316 16 -1.0889424425081273 2316 17 -1.2067991202266555 2316 18 -1.6501257200866064 2316 19 -1.6259350621678976 2316 20 -1.5641011500158086 2316 21 -1.2559328169887607 2316 22 -1.1798483922452832 2316 23 -1.1138575697428692 2316 24 -0.8770562692997714 2316 25 -0.9912358067952596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95355.0 3 3.0 4 858.0 5 4338.0 6 4977.0 7 4584.0 8 4334.0 9 4244.0 10 4014.0 11 3879.0 12 3984.0 13 3914.0 14 3950.0 15 4105.0 16 4341.0 17 4637.0 18 5217.0 19 6514.0 20 8565.0 21 11173.0 22 13870.0 23 16578.0 24 19225.0 25 22173.0 26 24765.0 27 27977.0 28 32397.0 29 38837.0 30 48225.0 31 61347.0 32 77581.0 33 100741.0 34 133301.0 35 182844.0 36 254324.0 37 318356.0 38 690037.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.641447761007925 19.0016405678039 13.181321040059423 25.175590631128753 2 15.601757928219543 20.476558218565724 41.29985975622132 22.62182409699341 3 19.181773487640523 23.87480383547296 29.53903785418719 27.404384822699328 4 12.256742626796653 16.543238135274702 38.49656478283407 32.70345445509458 5 12.909407168978484 37.82150052280853 36.11524766161196 13.153844646601032 6 31.75307562204918 37.203203474230506 18.05156125727101 12.99215964644931 7 28.218246856662375 31.520131968518527 21.863476358609486 18.398144816209612 8 24.35401529415327 38.490018543225666 20.306702458714156 16.849263703906903 9 25.965236350422433 15.997094716516653 20.646127209021873 37.39154172403904 10 15.100237456067065 29.231812700069653 34.31502920549302 21.352920638370264 11 33.93542479957747 22.612097228865117 24.988042784120818 18.464435187436596 12 23.714330755507977 24.491196413191886 31.80063609911461 19.993836732185528 13 29.01061826783828 22.212370700634672 25.825182448596433 22.951828582930613 14 20.40026559088405 22.93994502037132 27.56369349458197 29.096095894162655 15 22.361776373329818 31.97098813482938 23.440124619026665 22.227110872814134 16 21.37307153125006 28.600788042558573 28.70806621410033 21.318074212091037 17 21.09552085402229 29.271781407059795 28.388394531079523 21.244303207838396 18 20.879641135134605 27.735199986460618 30.926619225387054 20.458539653017723 19 23.188695588277863 27.064010385237996 27.896833437616202 21.850460588867936 20 23.09339658099257 27.752048037820344 28.73861533227287 20.415940048914216 21 22.198029670291987 27.215507539599653 27.59013828648742 22.99632450362094 22 22.54809009029069 28.857394150588394 27.80049208982309 20.794023669297825 23 21.61947679428618 28.42859706711592 28.41287675857035 21.539049380027546 24 22.768333727484922 26.897839915664925 29.34505366474134 20.988772692108817 25 22.21063315357411 27.821320907397357 28.79054213237577 21.17750380665277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 338.0 1 338.0 2 413.5 3 489.0 4 489.0 5 489.0 6 1265.0 7 2041.0 8 2041.0 9 2041.0 10 3258.0 11 4475.0 12 4475.0 13 4475.0 14 7332.0 15 10189.0 16 10189.0 17 10189.0 18 18524.0 19 26859.0 20 26859.0 21 26859.0 22 43129.0 23 59399.0 24 59399.0 25 59399.0 26 86402.5 27 113406.0 28 113406.0 29 113406.0 30 149106.5 31 184807.0 32 184807.0 33 184807.0 34 207362.5 35 229918.0 36 229918.0 37 229918.0 38 245154.5 39 260391.0 40 260391.0 41 260391.0 42 272777.0 43 285163.0 44 285163.0 45 285163.0 46 288675.0 47 292187.0 48 292187.0 49 292187.0 50 277670.0 51 263153.0 52 263153.0 53 263153.0 54 232801.0 55 202449.0 56 202449.0 57 202449.0 58 171711.5 59 140974.0 60 140974.0 61 140974.0 62 114705.5 63 88437.0 64 88437.0 65 88437.0 66 67718.5 67 47000.0 68 47000.0 69 47000.0 70 34167.5 71 21335.0 72 21335.0 73 21335.0 74 14801.0 75 8267.0 76 8267.0 77 8267.0 78 5637.5 79 3008.0 80 3008.0 81 3008.0 82 1968.0 83 928.0 84 928.0 85 928.0 86 585.5 87 243.0 88 243.0 89 243.0 90 161.5 91 80.0 92 80.0 93 80.0 94 51.0 95 22.0 96 22.0 97 22.0 98 14.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.851479628280468 3 0.0 4 0.0 5 0.0 6 8.906448446804455E-5 7 0.008015803602124009 8 0.0 9 0.0 10 0.004275095254466139 11 0.002582870049573292 12 8.906448446804455E-5 13 0.0 14 2.2266121117011137E-4 15 0.0 16 0.0 17 8.906448446804455E-5 18 0.011667447465313837 19 0.003072724714147537 20 0.0 21 0.006813433061805409 22 0.001736757447126869 23 0.002716466776275359 24 0.010465076924995235 25 0.006813433061805409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2245564.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.83005495644046 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2954937674401 64.57496541110709 2 9.783635274857561 14.642199305827214 3 2.127285428449189 4.775546565566635 4 0.7341251822196658 2.1973851092161785 5 0.33187548690187657 1.2417130461781432 6 0.17363010989259559 0.7795650399213436 7 0.11488039000808632 0.6017554128405689 8 0.07362851751379484 0.4407700809534806 9 0.053011824944629714 0.3570189996553059 >10 0.2747813548826211 3.603926902649847 >50 0.01984714969251405 1.0340825447050597 >100 0.014368335260203275 2.171420653195965 >500 0.0018090410195021288 0.9470981133450925 >1k 0.0014472328156006917 1.8930230144853437 >5k 1.8090410195008646E-4 0.7395298003527913 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5814 0.25891045634860554 No Hit GTATCAACGCAGAGTACTTTTTTTT 5372 0.2392272052811677 No Hit CTGTAGGACGTGGAATATGGCAAGA 5203 0.2317012563436179 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4352 0.19380431820246494 No Hit CTTTAGGACGTGAAATATGGCGAGG 4020 0.17901961378076955 No Hit GGTATCAACGCAGAGTACTTTTTTT 3351 0.14922754372620864 No Hit TATCAACGCAGAGTACTTTTTTTTT 3103 0.13818354765217114 No Hit GTCCTACAGTGTGCATTTCTCATTT 2554 0.1137353466656929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.4532242234022276E-5 0.0 5 0.0 4.4532242234022276E-5 0.0 4.4532242234022276E-5 0.0 6 0.0 4.4532242234022276E-5 0.0 4.4532242234022276E-5 0.0 7 0.0 4.4532242234022276E-5 0.0 4.4532242234022276E-5 0.0 8 0.0 4.4532242234022276E-5 0.0 4.4532242234022276E-5 0.0 9 0.0 4.4532242234022276E-5 0.0 4.4532242234022276E-5 0.0 10 0.0 4.4532242234022276E-5 0.0 4.4532242234022276E-5 0.0 11 0.0 4.4532242234022276E-5 0.0 8.906448446804455E-5 0.0 12 0.0 4.4532242234022276E-5 0.0 8.906448446804455E-5 4.4532242234022276E-5 13 0.0 4.4532242234022276E-5 0.0 8.906448446804455E-5 4.4532242234022276E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACG 40 0.0055787712 14.132256 4 TAGGACC 830 0.0 13.053892 4 TGTAGGA 2895 0.0 11.972488 2 GGCCTAC 150 6.311893E-10 11.587533 1 TGTAGAA 890 0.0 11.373091 2 CTGTAGG 2940 0.0 11.337142 1 GTAGGAC 3005 0.0 11.318346 3 GTCCTAC 2480 0.0 11.09006 1 AGGAGCG 85 5.7944693E-5 11.084123 5 CGCTGTC 60 0.0062512853 10.991755 7 GACGTGG 1675 0.0 10.912071 7 GGTATCA 1370 0.0 10.895963 1 CGTGGAA 1690 0.0 10.703244 9 GGACGTG 3135 0.0 10.608584 6 AGGACGT 3180 0.0 10.576972 5 GGCGAGG 1260 0.0 10.543582 19 TCCTACA 2745 0.0 10.540894 2 GGTTTAC 195 4.0017767E-11 10.486455 1 TAGGACG 3310 0.0 10.4462 4 AGGACCT 1715 0.0 10.437819 5 >>END_MODULE