FastQCFastQC Report
Thu 26 May 2016
SRR1512219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245564
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73190.32593148091080903No Hit
GTCCTACAGTGGACATTTCTAAATT65760.2928440249309305No Hit
CTGTAGGACGTGGAATATGGCAAGA58730.2615378586404128No Hit
GGTATCAACGCAGAGTACTTTTTTT46710.20801010347511806No Hit
GTCCTAAAGTGTGTATTTCTCATTT45480.20253263768033333No Hit
CTTTAGGACGTGAAATATGGCGAGG44430.197856752245761No Hit
TATCAACGCAGAGTACTTTTTTTTT44100.19638718825203827No Hit
GTCCTACAGTGTGCATTTCTCATTT28770.1281192609072821No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23310.10380465664750593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCG400.00529022114.24587511
TCCAACG2850.013.99594718
CTGATCG500.001503185313.2961519
CCGGTTT1850.012.83412212
TAGGACC8400.012.6672324
AGGAGCG701.0908634E-412.214835
AACACCG658.022861E-411.6928295
GCCGGTT1950.011.68892311
CCAACGA2050.011.58227119
ACTATCG1157.1071554E-811.5618711
GGCGAGG12300.011.50505419
GGTATCA16300.011.2334691
TGTAGGA27350.011.2198782
AGGACGT28650.011.20817855
CGGTTTC2300.011.14894613
GGACGTG28350.011.08856
AGGACCG1303.2661774E-810.9620285
CGTTTCC1658.0035534E-1110.93622814
CTGTAGG27250.010.9191381
GTAGGAC26550.010.8780743
Produced by FastQC (version 0.11.5)