FastQCFastQC Report
Thu 26 May 2016
SRR1512218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648750
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67600.25521472392638034No Hit
CTGTAGGACGTGGAATATGGCAAGA60110.22693723454459652No Hit
GTCCTAAAGTGTGTATTTCTCATTT48930.1847286455875413No Hit
GTATCAACGCAGAGTACTTTTTTTT44100.1664936290703162No Hit
CTTTAGGACGTGAAATATGGCGAGG43740.16513449740443606No Hit
GTCCTACAGTGTGCATTTCTCATTT33240.12549315714959888No Hit
GGTATCAACGCAGAGTACTTTTTTT27110.10235016517225107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11650.012.6984324
TAAGACG701.17790114E-412.1149274
TGTAGGA35150.011.7936582
TCCAACG3400.011.64027318
CTGTAGG34300.011.4607871
GTAGGAC36200.011.3228843
GTCCTAC30400.011.1509611
GACGTGG19000.011.1091027
CCAACGA2300.011.06187319
GGTATCA14250.010.9628731
GGACGTG34600.010.8390586
GTCCTAG4250.010.8111381
GGCGAGG14150.010.78825919
AGGACGT35400.010.7269965
GGCCTAC2101.8189894E-1210.6967331
TGTAGAA10600.010.5958212
AGGACCT23750.010.4344245
TAGGACG36850.010.4327554
TGGCGAG34200.010.19455818
GTCCTAA16550.010.1796711
Produced by FastQC (version 0.11.5)