FastQCFastQC Report
Thu 26 May 2016
SRR1512218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648750
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76610.28923076923076924No Hit
CTGTAGGACGTGGAATATGGCAAGA68560.25883907503539405No Hit
GTATCAACGCAGAGTACTTTTTTTT60110.22693723454459652No Hit
GTCCTAAAGTGTGTATTTCTCATTT52820.19941481831052382No Hit
CTTTAGGACGTGAAATATGGCGAGG51520.19450684285040112No Hit
GGTATCAACGCAGAGTACTTTTTTT39010.14727701746106653No Hit
TATCAACGCAGAGTACTTTTTTTTT36790.1388957055214724No Hit
GTCCTACAGTGTGCATTTCTCATTT36240.13681925436526662No Hit
CTGTAGGACCTGGAATATGGCGAGA29310.1106559697970741No Hit
CTGAAGGACCTGGAATATGGCGAGA27390.10340726757904672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGT250.006043015518.9946386
TAGGACC11500.012.3087084
GCCGTCC658.045721E-411.6890098
TGTAGGA37000.011.168932
TTCGCAT600.005889869811.08020617
GATATAC8350.010.9646081
CTGTAGG36250.010.9181351
GTAGGAC36050.010.8835063
GTCCTAC35300.010.7796971
ATGACCG803.772529E-410.68751055
CGTGCGC803.7824138E-410.684484510
GACGTGG19300.010.6783337
GGCGAGG13950.010.6214619
AGGACGT34200.010.5000115
ACCTTTT8350.010.46411315
TCCTACA38200.010.4450372
GGTATCA16450.010.3775661
TGGACCG1106.0491784E-610.3636475
GGACGTG34200.010.3581896
TAGGACG36700.010.198924
Produced by FastQC (version 0.11.5)