FastQCFastQC Report
Thu 26 May 2016
SRR1512217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987825
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51530.25922805075899535No Hit
GTATCAACGCAGAGTACTTTTTTTT46330.23306880635870866No Hit
CTGTAGGACGTGGAATATGGCAAGA45340.2280884886748079No Hit
GTCCTAAAGTGTGTATTTCTCATTT31060.15625117905248198No Hit
GGTATCAACGCAGAGTACTTTTTTT29470.148252487014702No Hit
GTCCTACAGTGTGCATTTCTCATTT26380.1327078590922239No Hit
CTTTAGGACGTGAAATATGGCGAGG25860.13009193465219523No Hit
TATCAACGCAGAGTACTTTTTTTTT25190.12672141662369676No Hit
CTGTAGGACCTGGAATATGGCGAGA19990.10056217222341002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2400.013.7426318
CCAACGA1400.013.46216919
CGGTAGG1005.5981218E-813.278861
TAGGACC9300.012.7654214
CTAACCC1501.8189894E-1212.5627953
TATGTCG1459.094947E-1212.34712616
TGTAGGA24200.012.2827352
GTCCTAA9900.012.2780451
TCCTACG751.0557921E-412.2574092
CTAGTAC701.1783463E-412.1141233
GGACCGG550.003264114711.9917576
AGGACCG951.1489701E-611.9015955
GTAGGAC25750.011.8553753
CTGTAGG24300.011.8118381
GTCCTAC24850.011.7148271
GGCGAGG8800.011.67231219
GACGTGG12500.011.2311387
GTAGCAC1355.3805707E-911.1669283
GGACGTG24000.011.0709636
TCCTACA26800.011.0530122
Produced by FastQC (version 0.11.5)