##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512217_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1987825 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.502172978003596 31.0 28.0 33.0 16.0 34.0 2 25.624945354847636 31.0 25.0 33.0 2.0 34.0 3 27.278289084803742 31.0 26.0 34.0 16.0 34.0 4 31.711483153698136 35.0 32.0 37.0 19.0 37.0 5 32.11679398337379 35.0 33.0 37.0 22.0 37.0 6 32.31986719152843 35.0 33.0 37.0 23.0 37.0 7 32.29273703671098 35.0 33.0 37.0 22.0 37.0 8 32.2903600669073 35.0 33.0 37.0 22.0 37.0 9 33.706751348836036 38.0 34.0 39.0 22.0 39.0 10 33.55668532189705 38.0 33.0 39.0 22.0 39.0 11 33.5717802120408 38.0 34.0 39.0 22.0 39.0 12 33.42081521260674 38.0 33.0 39.0 19.0 39.0 13 33.42201501641241 38.0 33.0 39.0 19.0 39.0 14 34.37717404701118 39.0 34.0 41.0 18.0 41.0 15 34.30685045212733 39.0 33.0 41.0 18.0 41.0 16 34.24394551834291 39.0 33.0 41.0 17.0 41.0 17 34.21709255090362 39.0 33.0 41.0 17.0 41.0 18 34.157958069749604 39.0 33.0 41.0 17.0 41.0 19 34.170744406575025 39.0 34.0 41.0 18.0 41.0 20 34.07117729176361 39.0 34.0 41.0 17.0 41.0 21 33.9776635267189 39.0 34.0 41.0 13.0 41.0 22 33.864423176084415 39.0 33.0 41.0 10.0 41.0 23 33.723651729905804 39.0 33.0 41.0 8.0 41.0 24 33.587970772075 39.0 33.0 41.0 2.0 41.0 25 33.43921673185517 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6265663925735083 1101 2 3.6095851869663598 1101 3 2.157191371999847 1101 4 0.9454367181409076 1101 5 0.7342669987400967 1101 6 0.5790757977421634 1101 7 0.32664442772542657 1101 8 0.2747325021458096 1101 9 0.4836811582589249 1101 10 0.2700584388460072 1101 11 0.5288493692363474 1101 12 0.5512370386400818 1101 13 0.44786662355293316 1101 14 0.5439693615756127 1101 15 0.43469186749618416 1101 16 0.7778957357101319 1101 17 0.5212422944711079 1101 18 0.680754912917223 1101 19 0.6337439207003541 1101 20 0.5880687882733824 1101 21 0.6412664789965774 1101 22 0.6613480755629908 1101 23 0.8173036340995239 1101 24 0.8410062488414596 1101 25 0.7622897203664039 1102 1 1.4703164565044844 1102 2 4.093426212620873 1102 3 2.6370319990173243 1102 4 1.7274126543753532 1102 5 1.324118880531941 1102 6 1.465036380021111 1102 7 1.2181008820360404 1102 8 1.0893119210768063 1102 9 1.0999146870822258 1102 10 1.2278829912049645 1102 11 1.2287841084958657 1102 12 1.2842227043653978 1102 13 1.2332113934437956 1102 14 1.3958590443289225 1102 15 1.5373762670812923 1102 16 1.3757808551799542 1102 17 1.5022973908439496 1102 18 1.6010780661011026 1102 19 1.527070812713852 1102 20 1.4856775213470215 1102 21 1.6204575672961425 1102 22 1.4312330933277622 1102 23 1.603188492877095 1102 24 1.5860918929690584 1102 25 1.4115262456893518 1103 1 1.1606454857184012 1103 2 3.96812785600423 1103 3 2.4497279244453054 1103 4 1.5174079528701618 1103 5 1.1316857373740703 1103 6 0.9696201360527539 1103 7 0.8770809966715376 1103 8 1.0042361117734657 1103 9 1.0516835050189002 1103 10 1.113558009741638 1103 11 1.050820996838361 1103 12 1.0133689059468693 1103 13 1.056328753674137 1103 14 1.3477835449808424 1103 15 1.4106279741620114 1103 16 1.2822186456925024 1103 17 1.4242498664388563 1103 18 1.1163757260939988 1103 19 1.3610710152141934 1103 20 1.3272100033328087 1103 21 1.4464434943683813 1103 22 1.3813974203821715 1103 23 1.419214392614606 1103 24 1.5972426674482634 1103 25 1.3334062385708165 1104 1 1.1479105319608038 1104 2 3.865559190858651 1104 3 2.4153654177101664 1104 4 1.4990390424879614 1104 5 1.3622985185037066 1104 6 1.083102218923223 1104 7 1.075537513734993 1104 8 1.0591892928649642 1104 9 1.0366691367249388 1104 10 0.8613733860335131 1104 11 0.8579066943990767 1104 12 0.8620641739443826 1104 13 1.009449700118978 1104 14 1.2108623510820493 1104 15 0.7928193274646134 1104 16 1.0557486672227725 1104 17 0.9437789943854042 1104 18 1.1029805625327 1104 19 1.209211673716652 1104 20 1.1976328812932238 1104 21 1.3222730526985558 1104 22 1.2573106828940084 1104 23 1.4384231164750645 1104 24 1.569314296054472 1104 25 1.2501369306214656 1105 1 1.2697832182221482 1105 2 3.9656310781806567 1105 3 2.5857986356794136 1105 4 1.7789734399258457 1105 5 1.312037657922069 1105 6 1.1107874471547063 1105 7 0.8211876850592716 1105 8 0.9037426444499346 1105 9 1.0032200088429875 1105 10 1.1637413630295725 1105 11 1.1205623067757529 1105 12 0.8929821793509944 1105 13 0.9981586554713573 1105 14 1.0107831458889436 1105 15 1.0454580656914487 1105 16 0.9143049803932186 1105 17 1.0352244030669837 1105 18 1.1042181436749772 1105 19 1.061168989004976 1105 20 1.0433657015146522 1105 21 1.0080156874765223 1105 22 1.1999062887276466 1105 23 0.9855609679481105 1105 24 0.9266717697531845 1105 25 1.049784381419549 1106 1 0.12688262068618883 1106 2 2.924254261047043 1106 3 1.3670291265762273 1106 4 0.4990552348270434 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-2.3215811052012434 2315 20 -2.127889088079016 2315 21 -2.093629563597055 2315 22 -1.9224643316156786 2315 23 -1.9449209015510895 2315 24 -1.8735877170933897 2315 25 -1.8509325186173875 2316 1 -1.6812561573765414 2316 2 -0.08989972715147942 2316 3 -0.6419836907880736 2316 4 -1.3072295814743988 2316 5 -1.2482659269805652 2316 6 -1.4090421642801623 2316 7 -1.5755670924027747 2316 8 -1.6060872077979234 2316 9 -1.5208719924199414 2316 10 -1.6165562550624415 2316 11 -2.119580415053143 2316 12 -1.7384950210107597 2316 13 -1.6944429007156643 2316 14 -1.8245302053180694 2316 15 -2.1222728605823207 2316 16 -1.7690072149114684 2316 17 -2.049826060631794 2316 18 -2.325182178479281 2316 19 -2.287375028932914 2316 20 -2.305397054884285 2316 21 -2.122500267721559 2316 22 -2.0748941040650912 2316 23 -1.920441028123964 2316 24 -2.111254937477092 2316 25 -1.956729609814861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 106807.0 3 2.0 4 948.0 5 4534.0 6 5459.0 7 4764.0 8 4555.0 9 4219.0 10 4103.0 11 4006.0 12 3979.0 13 4059.0 14 3924.0 15 4061.0 16 4413.0 17 4622.0 18 5248.0 19 6565.0 20 8638.0 21 11090.0 22 13542.0 23 16320.0 24 18900.0 25 21062.0 26 23484.0 27 26498.0 28 30533.0 29 36121.0 30 44270.0 31 55580.0 32 69349.0 33 89630.0 34 117057.0 35 158575.0 36 219432.0 37 274470.0 38 577006.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.480487467458154 18.60319696150315 12.66781532579578 25.248500245242916 2 15.804108627616289 19.988155425682113 41.21066600645344 22.997069940248156 3 19.981789141398263 22.879176989926176 29.007784890521048 28.131248978154517 4 12.181102461232754 15.912014387584419 38.08780954057827 33.819073610604555 5 12.99571139310553 37.7621269477947 35.95774275904569 13.28441890005408 6 32.70267659780403 36.77552617890335 17.549609572688098 12.97218765060453 7 29.00080294776412 31.121571121249136 20.969866316240676 18.907759614746062 8 24.746947040106647 38.77509338095657 19.660080741513966 16.81787883742281 9 25.862135751386568 15.994919069837637 20.21365059801542 37.929294580760384 10 14.833972918096258 29.613311144068565 34.22923831391102 21.32347762392416 11 35.034510861362925 22.36157924921269 23.979816679914276 18.624093209510107 12 23.528313903357546 24.443033631783933 31.726281327000105 20.30237113785842 13 29.487957943984 21.760517148139296 25.517437400173552 23.234087507703144 14 20.19810646839251 22.730830759324284 27.327323399502973 29.743739372780233 15 22.657879843547597 31.925597072176874 23.049966672116508 22.36655641215902 16 21.53977337039226 28.70504194282696 28.46714373750204 21.288040949278734 17 21.169368530932047 29.265302529146176 28.261391219045944 21.303937720875833 18 20.940375627009324 27.836094606084693 30.756486433455255 20.467043333450725 19 23.11117863499573 27.162293097706282 27.712607167615634 22.013921099682356 20 23.077987247368355 27.74102348043716 28.75353715744595 20.427452114748533 21 22.199918499177446 27.30358050218593 27.413757540083814 23.082743458552805 22 22.293143910715138 28.820527267650842 27.85760101016453 21.02872781146949 23 21.81377579767539 28.31063459866705 28.22169097524067 21.653898628416883 24 22.658588688213978 26.89313784383984 29.35034055702632 21.097932910919862 25 22.203562929833122 27.888956527426306 28.706739984605246 21.200740558135323 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 295.5 3 405.0 4 405.0 5 405.0 6 1094.5 7 1784.0 8 1784.0 9 1784.0 10 2868.0 11 3952.0 12 3952.0 13 3952.0 14 6475.0 15 8998.0 16 8998.0 17 8998.0 18 15383.0 19 21768.0 20 21768.0 21 21768.0 22 35265.0 23 48762.0 24 48762.0 25 48762.0 26 70676.5 27 92591.0 28 92591.0 29 92591.0 30 123193.5 31 153796.0 32 153796.0 33 153796.0 34 175145.5 35 196495.0 36 196495.0 37 196495.0 38 212020.5 39 227546.0 40 227546.0 41 227546.0 42 241038.5 43 254531.0 44 254531.0 45 254531.0 46 259144.5 47 263758.0 48 263758.0 49 263758.0 50 252409.0 51 241060.0 52 241060.0 53 241060.0 54 212892.0 55 184724.0 56 184724.0 57 184724.0 58 157629.0 59 130534.0 60 130534.0 61 130534.0 62 105802.5 63 81071.0 64 81071.0 65 81071.0 66 62222.0 67 43373.0 68 43373.0 69 43373.0 70 31806.5 71 20240.0 72 20240.0 73 20240.0 74 14179.0 75 8118.0 76 8118.0 77 8118.0 78 5473.0 79 2828.0 80 2828.0 81 2828.0 82 1878.0 83 928.0 84 928.0 85 928.0 86 604.5 87 281.0 88 281.0 89 281.0 90 176.5 91 72.0 92 72.0 93 72.0 94 46.5 95 21.0 96 21.0 97 21.0 98 12.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.7508835033265004 3 0.0 4 0.0 5 0.0 6 1.50918717693962E-4 7 0.007495629645466778 8 0.0 9 0.0 10 0.003873580420811691 11 0.00226378076540943 12 1.50918717693962E-4 13 0.0 14 2.515311961566033E-4 15 0.0 16 0.0 17 0.0 18 0.010815841434733943 19 0.002565618200797354 20 0.0 21 0.005785217511601877 22 0.0015091871769396198 23 0.0024650057223347126 24 0.010010941607032812 25 0.00679134229622829 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1987825.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.17951203428238 #Duplication Level Percentage of deduplicated Percentage of total 1 87.72708516135248 67.7073362494312 2 8.908093669487938 13.75044645133518 3 1.8296956039108838 4.236450416433409 4 0.6059312062636821 1.8706189930310035 5 0.2783469699211735 1.0741341657368624 6 0.16335145323682096 0.7564431270545243 7 0.09928317843596458 0.5363839085430234 8 0.0663167360025666 0.40946346595075334 9 0.04966039921789799 0.3449488841158523 >10 0.23974906200973536 3.2366523577433823 >50 0.016312749625459384 0.8710052925903378 >100 0.013006602922239263 1.9378047321831273 >500 0.0018475377743703116 1.0477970409118902 >1k 0.001253686346894118 1.9580945916433379 >5k 6.598349194179568E-5 0.26242032329618 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5153 0.25922805075899535 No Hit GTATCAACGCAGAGTACTTTTTTTT 4633 0.23306880635870866 No Hit CTGTAGGACGTGGAATATGGCAAGA 4534 0.2280884886748079 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3106 0.15625117905248198 No Hit GGTATCAACGCAGAGTACTTTTTTT 2947 0.148252487014702 No Hit GTCCTACAGTGTGCATTTCTCATTT 2638 0.1327078590922239 No Hit CTTTAGGACGTGAAATATGGCGAGG 2586 0.13009193465219523 No Hit TATCAACGCAGAGTACTTTTTTTTT 2519 0.12672141662369676 No Hit CTGTAGGACCTGGAATATGGCGAGA 1999 0.10056217222341002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 5.030623923132066E-5 0.0 12 0.0 0.0 0.0 5.030623923132066E-5 5.030623923132066E-5 13 0.0 0.0 0.0 5.030623923132066E-5 5.030623923132066E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 240 0.0 13.74263 18 CCAACGA 140 0.0 13.462169 19 CGGTAGG 100 5.5981218E-8 13.27886 1 TAGGACC 930 0.0 12.765421 4 CTAACCC 150 1.8189894E-12 12.562795 3 TATGTCG 145 9.094947E-12 12.347126 16 TGTAGGA 2420 0.0 12.282735 2 GTCCTAA 990 0.0 12.278045 1 TCCTACG 75 1.0557921E-4 12.257409 2 CTAGTAC 70 1.1783463E-4 12.114123 3 GGACCGG 55 0.0032641147 11.991757 6 AGGACCG 95 1.1489701E-6 11.901595 5 GTAGGAC 2575 0.0 11.855375 3 CTGTAGG 2430 0.0 11.811838 1 GTCCTAC 2485 0.0 11.714827 1 GGCGAGG 880 0.0 11.672312 19 GACGTGG 1250 0.0 11.231138 7 GTAGCAC 135 5.3805707E-9 11.166928 3 GGACGTG 2400 0.0 11.070963 6 TCCTACA 2680 0.0 11.053012 2 >>END_MODULE